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Nucleic Acid Aptamers: An Emerging Tool for Biotechnology and Biomedical Sensing.

Ku TH, Zhang T, Luo H, Yen TM, Chen PW, Han Y, Lo YH - Sensors (Basel) (2015)

Bottom Line: Detection of small molecules or proteins of living cells provides an exceptional opportunity to study genetic variations and functions, cellular behaviors, and various diseases including cancer and microbial infections.Our aim in this review is to give an overview of selected research activities related to nucleic acid-based aptamer techniques that have been reported in the past two decades.Limitations of aptamers and possible approaches to overcome these limitations are also discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical and Computer Engineering, University of California San Diego, La Jolla, CA 92093-0407, USA. tiku@eng.ucsd.edu.

ABSTRACT
Detection of small molecules or proteins of living cells provides an exceptional opportunity to study genetic variations and functions, cellular behaviors, and various diseases including cancer and microbial infections. Our aim in this review is to give an overview of selected research activities related to nucleic acid-based aptamer techniques that have been reported in the past two decades. Limitations of aptamers and possible approaches to overcome these limitations are also discussed.

No MeSH data available.


Related in: MedlinePlus

The annual trends in the number of publications of aptamers-related research. The term “aptamer” has been utilized for literature search on Thomson Reuters Web of Science.
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sensors-15-16281-f001: The annual trends in the number of publications of aptamers-related research. The term “aptamer” has been utilized for literature search on Thomson Reuters Web of Science.

Mentions: Aptamers are short single-stranded DNA- or RNA-based oligonucleotides that can selectively bind to small molecular ligands or protein targets with high affinity and specificity, when folded into their unique three-dimensional structures. They have been first reported more than 20 years ago by Ellington and Gold [1,2]. Ellington reported a selection of RNA molecules that specifically bind to organic dyes, and Gold reported an RNA ligand that interacts with T4 DNA polymerase. Several aptamers have been identified for different targets as diagnostic tools and disease treatment drugs. There is an intense interest and ever increasing need within basic and clinical sciences to detect, analyze and quantify these small molecules and proteins utilizing aptamers. Over 1000 papers related to aptamers have been published each year since 2010, attesting the wide applicability and great potential of aptamers. Figure 1 indicates a large number of scientific publications in this active field of research.


Nucleic Acid Aptamers: An Emerging Tool for Biotechnology and Biomedical Sensing.

Ku TH, Zhang T, Luo H, Yen TM, Chen PW, Han Y, Lo YH - Sensors (Basel) (2015)

The annual trends in the number of publications of aptamers-related research. The term “aptamer” has been utilized for literature search on Thomson Reuters Web of Science.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4541879&req=5

sensors-15-16281-f001: The annual trends in the number of publications of aptamers-related research. The term “aptamer” has been utilized for literature search on Thomson Reuters Web of Science.
Mentions: Aptamers are short single-stranded DNA- or RNA-based oligonucleotides that can selectively bind to small molecular ligands or protein targets with high affinity and specificity, when folded into their unique three-dimensional structures. They have been first reported more than 20 years ago by Ellington and Gold [1,2]. Ellington reported a selection of RNA molecules that specifically bind to organic dyes, and Gold reported an RNA ligand that interacts with T4 DNA polymerase. Several aptamers have been identified for different targets as diagnostic tools and disease treatment drugs. There is an intense interest and ever increasing need within basic and clinical sciences to detect, analyze and quantify these small molecules and proteins utilizing aptamers. Over 1000 papers related to aptamers have been published each year since 2010, attesting the wide applicability and great potential of aptamers. Figure 1 indicates a large number of scientific publications in this active field of research.

Bottom Line: Detection of small molecules or proteins of living cells provides an exceptional opportunity to study genetic variations and functions, cellular behaviors, and various diseases including cancer and microbial infections.Our aim in this review is to give an overview of selected research activities related to nucleic acid-based aptamer techniques that have been reported in the past two decades.Limitations of aptamers and possible approaches to overcome these limitations are also discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical and Computer Engineering, University of California San Diego, La Jolla, CA 92093-0407, USA. tiku@eng.ucsd.edu.

ABSTRACT
Detection of small molecules or proteins of living cells provides an exceptional opportunity to study genetic variations and functions, cellular behaviors, and various diseases including cancer and microbial infections. Our aim in this review is to give an overview of selected research activities related to nucleic acid-based aptamer techniques that have been reported in the past two decades. Limitations of aptamers and possible approaches to overcome these limitations are also discussed.

No MeSH data available.


Related in: MedlinePlus