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A Sensitive and Selective Label-Free Electrochemical DNA Biosensor for the Detection of Specific Dengue Virus Serotype 3 Sequences.

Oliveira N, Souza E, Ferreira D, Zanforlin D, Bezerra W, Borba MA, Arruda M, Lopes K, Nascimento G, Martins D, Cordeiro M, Lima-Filho J - Sensors (Basel) (2015)

Bottom Line: Dengue fever is the most prevalent vector-borne disease in the world, with nearly 100 million people infected every year.Early diagnosis and identification of the pathogen are crucial steps for the treatment and for prevention of the disease, mainly in areas where the co-circulation of different serotypes is common, increasing the outcome of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS).Moreover, this system allows selective detection of DENV-3 sequences in buffer and human serum solutions.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego, s/n, Campus da UFPE, 50670-901 Recife, PE, Brazil. nataliacybelle.89@gmail.com.

ABSTRACT
Dengue fever is the most prevalent vector-borne disease in the world, with nearly 100 million people infected every year. Early diagnosis and identification of the pathogen are crucial steps for the treatment and for prevention of the disease, mainly in areas where the co-circulation of different serotypes is common, increasing the outcome of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Due to the lack of fast and inexpensive methods available for the identification of dengue serotypes, herein we report the development of an electrochemical DNA biosensor for the detection of sequences of dengue virus serotype 3 (DENV-3). DENV-3 probe was designed using bioinformatics software and differential pulse voltammetry (DPV) was used for electrochemical analysis. The results showed that a 22-m sequence was the best DNA probe for the identification of DENV-3. The optimum concentration of the DNA probe immobilized onto the electrode surface is 500 nM and a low detection limit of the system (3.09 nM). Moreover, this system allows selective detection of DENV-3 sequences in buffer and human serum solutions. Therefore, the application of DNA biosensors for diagnostics at the molecular level may contribute to future advances in the implementation of specific, effective and rapid detection methods for the diagnosis dengue viruses.

No MeSH data available.


Related in: MedlinePlus

Differential pulse voltammograms for guanine oxidation of (a) bare PGE; (b) probe-modified PGE; (c) probe-modified PGE after hybridization with DENV-3 sequence; (d) non-complementary sequence and (e) a mixed solution of DENV-3 sequence and non-complementary sequence. Experimental conditions: Scanning potential range between +0.5 V and +1.2 V and scan rate of 0.05 V/s.
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sensors-15-15562-f005: Differential pulse voltammograms for guanine oxidation of (a) bare PGE; (b) probe-modified PGE; (c) probe-modified PGE after hybridization with DENV-3 sequence; (d) non-complementary sequence and (e) a mixed solution of DENV-3 sequence and non-complementary sequence. Experimental conditions: Scanning potential range between +0.5 V and +1.2 V and scan rate of 0.05 V/s.

Mentions: In a way to evaluate the selectivity of the DENV-3 biosensor, hybridization tests were performed with a non-complementary sequence. DPV voltammograms for bare PGE, probe-modified PGE before and after hybridization with DENV-3 target and non-complementary sequence are displayed in Figure 5. It was verified that no electrochemical signal was recorded with bare PGE, which is in agreement with the absence of DNA on the electrode surface. Probe-modified PGE presented the highest current peak of the system, whereas the probe-modified PGE after hybridization with target sequence showed a decrease in the current signal, as discussed previously.


A Sensitive and Selective Label-Free Electrochemical DNA Biosensor for the Detection of Specific Dengue Virus Serotype 3 Sequences.

Oliveira N, Souza E, Ferreira D, Zanforlin D, Bezerra W, Borba MA, Arruda M, Lopes K, Nascimento G, Martins D, Cordeiro M, Lima-Filho J - Sensors (Basel) (2015)

Differential pulse voltammograms for guanine oxidation of (a) bare PGE; (b) probe-modified PGE; (c) probe-modified PGE after hybridization with DENV-3 sequence; (d) non-complementary sequence and (e) a mixed solution of DENV-3 sequence and non-complementary sequence. Experimental conditions: Scanning potential range between +0.5 V and +1.2 V and scan rate of 0.05 V/s.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4541844&req=5

sensors-15-15562-f005: Differential pulse voltammograms for guanine oxidation of (a) bare PGE; (b) probe-modified PGE; (c) probe-modified PGE after hybridization with DENV-3 sequence; (d) non-complementary sequence and (e) a mixed solution of DENV-3 sequence and non-complementary sequence. Experimental conditions: Scanning potential range between +0.5 V and +1.2 V and scan rate of 0.05 V/s.
Mentions: In a way to evaluate the selectivity of the DENV-3 biosensor, hybridization tests were performed with a non-complementary sequence. DPV voltammograms for bare PGE, probe-modified PGE before and after hybridization with DENV-3 target and non-complementary sequence are displayed in Figure 5. It was verified that no electrochemical signal was recorded with bare PGE, which is in agreement with the absence of DNA on the electrode surface. Probe-modified PGE presented the highest current peak of the system, whereas the probe-modified PGE after hybridization with target sequence showed a decrease in the current signal, as discussed previously.

Bottom Line: Dengue fever is the most prevalent vector-borne disease in the world, with nearly 100 million people infected every year.Early diagnosis and identification of the pathogen are crucial steps for the treatment and for prevention of the disease, mainly in areas where the co-circulation of different serotypes is common, increasing the outcome of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS).Moreover, this system allows selective detection of DENV-3 sequences in buffer and human serum solutions.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego, s/n, Campus da UFPE, 50670-901 Recife, PE, Brazil. nataliacybelle.89@gmail.com.

ABSTRACT
Dengue fever is the most prevalent vector-borne disease in the world, with nearly 100 million people infected every year. Early diagnosis and identification of the pathogen are crucial steps for the treatment and for prevention of the disease, mainly in areas where the co-circulation of different serotypes is common, increasing the outcome of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Due to the lack of fast and inexpensive methods available for the identification of dengue serotypes, herein we report the development of an electrochemical DNA biosensor for the detection of sequences of dengue virus serotype 3 (DENV-3). DENV-3 probe was designed using bioinformatics software and differential pulse voltammetry (DPV) was used for electrochemical analysis. The results showed that a 22-m sequence was the best DNA probe for the identification of DENV-3. The optimum concentration of the DNA probe immobilized onto the electrode surface is 500 nM and a low detection limit of the system (3.09 nM). Moreover, this system allows selective detection of DENV-3 sequences in buffer and human serum solutions. Therefore, the application of DNA biosensors for diagnostics at the molecular level may contribute to future advances in the implementation of specific, effective and rapid detection methods for the diagnosis dengue viruses.

No MeSH data available.


Related in: MedlinePlus