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A Sensitive and Selective Label-Free Electrochemical DNA Biosensor for the Detection of Specific Dengue Virus Serotype 3 Sequences.

Oliveira N, Souza E, Ferreira D, Zanforlin D, Bezerra W, Borba MA, Arruda M, Lopes K, Nascimento G, Martins D, Cordeiro M, Lima-Filho J - Sensors (Basel) (2015)

Bottom Line: Dengue fever is the most prevalent vector-borne disease in the world, with nearly 100 million people infected every year.Early diagnosis and identification of the pathogen are crucial steps for the treatment and for prevention of the disease, mainly in areas where the co-circulation of different serotypes is common, increasing the outcome of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS).Moreover, this system allows selective detection of DENV-3 sequences in buffer and human serum solutions.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego, s/n, Campus da UFPE, 50670-901 Recife, PE, Brazil. nataliacybelle.89@gmail.com.

ABSTRACT
Dengue fever is the most prevalent vector-borne disease in the world, with nearly 100 million people infected every year. Early diagnosis and identification of the pathogen are crucial steps for the treatment and for prevention of the disease, mainly in areas where the co-circulation of different serotypes is common, increasing the outcome of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Due to the lack of fast and inexpensive methods available for the identification of dengue serotypes, herein we report the development of an electrochemical DNA biosensor for the detection of sequences of dengue virus serotype 3 (DENV-3). DENV-3 probe was designed using bioinformatics software and differential pulse voltammetry (DPV) was used for electrochemical analysis. The results showed that a 22-m sequence was the best DNA probe for the identification of DENV-3. The optimum concentration of the DNA probe immobilized onto the electrode surface is 500 nM and a low detection limit of the system (3.09 nM). Moreover, this system allows selective detection of DENV-3 sequences in buffer and human serum solutions. Therefore, the application of DNA biosensors for diagnostics at the molecular level may contribute to future advances in the implementation of specific, effective and rapid detection methods for the diagnosis dengue viruses.

No MeSH data available.


Related in: MedlinePlus

Differential pulse voltammograms corresponding to bare PGE (a), probe-modified PGE before (b) and after hybridization with 250 nM of target sequence (c) in 20mM Tris-HCl buffer solution (pH 7.0). Experimental conditions: Scanning potential range between +0.5 V and +1.2 V and scan rate of 0.05 V/s.
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sensors-15-15562-f004: Differential pulse voltammograms corresponding to bare PGE (a), probe-modified PGE before (b) and after hybridization with 250 nM of target sequence (c) in 20mM Tris-HCl buffer solution (pH 7.0). Experimental conditions: Scanning potential range between +0.5 V and +1.2 V and scan rate of 0.05 V/s.

Mentions: Figure 4 displays electrochemical signals of probe-modified PGE before and after hybridization with 250 nM of the target. A decrease of 83% in the current signal was observed after the reaction with the DENV-3 target; this is due to the fact that oxidizable regions of guanine bases in the ssDNA (777 nA) are bound through hydrogen bonds that held the double chain together, thus decreasing the electrochemical signal of the dsDNA (135 nA) on the electrode surface [24,58,59,60].


A Sensitive and Selective Label-Free Electrochemical DNA Biosensor for the Detection of Specific Dengue Virus Serotype 3 Sequences.

Oliveira N, Souza E, Ferreira D, Zanforlin D, Bezerra W, Borba MA, Arruda M, Lopes K, Nascimento G, Martins D, Cordeiro M, Lima-Filho J - Sensors (Basel) (2015)

Differential pulse voltammograms corresponding to bare PGE (a), probe-modified PGE before (b) and after hybridization with 250 nM of target sequence (c) in 20mM Tris-HCl buffer solution (pH 7.0). Experimental conditions: Scanning potential range between +0.5 V and +1.2 V and scan rate of 0.05 V/s.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4541844&req=5

sensors-15-15562-f004: Differential pulse voltammograms corresponding to bare PGE (a), probe-modified PGE before (b) and after hybridization with 250 nM of target sequence (c) in 20mM Tris-HCl buffer solution (pH 7.0). Experimental conditions: Scanning potential range between +0.5 V and +1.2 V and scan rate of 0.05 V/s.
Mentions: Figure 4 displays electrochemical signals of probe-modified PGE before and after hybridization with 250 nM of the target. A decrease of 83% in the current signal was observed after the reaction with the DENV-3 target; this is due to the fact that oxidizable regions of guanine bases in the ssDNA (777 nA) are bound through hydrogen bonds that held the double chain together, thus decreasing the electrochemical signal of the dsDNA (135 nA) on the electrode surface [24,58,59,60].

Bottom Line: Dengue fever is the most prevalent vector-borne disease in the world, with nearly 100 million people infected every year.Early diagnosis and identification of the pathogen are crucial steps for the treatment and for prevention of the disease, mainly in areas where the co-circulation of different serotypes is common, increasing the outcome of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS).Moreover, this system allows selective detection of DENV-3 sequences in buffer and human serum solutions.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego, s/n, Campus da UFPE, 50670-901 Recife, PE, Brazil. nataliacybelle.89@gmail.com.

ABSTRACT
Dengue fever is the most prevalent vector-borne disease in the world, with nearly 100 million people infected every year. Early diagnosis and identification of the pathogen are crucial steps for the treatment and for prevention of the disease, mainly in areas where the co-circulation of different serotypes is common, increasing the outcome of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Due to the lack of fast and inexpensive methods available for the identification of dengue serotypes, herein we report the development of an electrochemical DNA biosensor for the detection of sequences of dengue virus serotype 3 (DENV-3). DENV-3 probe was designed using bioinformatics software and differential pulse voltammetry (DPV) was used for electrochemical analysis. The results showed that a 22-m sequence was the best DNA probe for the identification of DENV-3. The optimum concentration of the DNA probe immobilized onto the electrode surface is 500 nM and a low detection limit of the system (3.09 nM). Moreover, this system allows selective detection of DENV-3 sequences in buffer and human serum solutions. Therefore, the application of DNA biosensors for diagnostics at the molecular level may contribute to future advances in the implementation of specific, effective and rapid detection methods for the diagnosis dengue viruses.

No MeSH data available.


Related in: MedlinePlus