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Hydrocortisone supresses inflammatory activity of metalloproteinase-8 in carotid plaque.

Gabriel SA, Antonangelo L, Capelozzi VL, Beteli CB, Camargo Júnior Od, Aquino JL, Caffaro RA - Rev Bras Cir Cardiovasc (2015 Jul-Sep)

Bottom Line: Symptomatic patients in Group 1 exhibited lower tissue concentration of MMP-8 in comparison to the control group (143.89 pg/ml and 1317.36 respectively; P=0.003).There was a correlation between preoperative MMP-9 levels and tissue concentrations of MMP-8 (P=0.042) and MMP-9 (P=0.019) between symptomatic patients in the control group.There was an association between systemic and tissue inflammation.

View Article: PubMed Central - PubMed

Affiliation: Faculdade de Medicina, Pontifícia Universidade Católica de Campinas, Campinas, SP, BR.

ABSTRACT

Objective: Matrix metalloproteinases are inflammatory biomarkers involved in carotid plaque instability. Our objective was to analyze the inflammatory activity of plasma and carotid plaque MMP-8 and MMP-9 after intravenous administration of hydrocortisone.

Methods: The study included 22 patients with stenosis ≥ 70% in the carotid artery (11 symptomatic and 11 asymptomatic) who underwent carotid endarterectomy. The patients were divided into two groups: Control Group - hydrocortisone was not administered, and Group 1 - 500 mg intravenous hydrocortisone was administered during anesthetic induction. Plasma levels of MMP-8 and MMP-9 were measured preoperatively (24 hours before carotid endarterectomy) and at 1 hour, 6 hours and 24 hours after carotid endarterectomy. In carotid plaque, tissue levels of MMP-8 and MMP-9 were measured.

Results: Group 1 showed increased serum levels of MMP- 8 (994.28 pg/ml and 408.54 pg/ml, respectively; P=0.045) and MMP-9 (106,656.34 and 42,807.69 respectively; P=0.014) at 1 hour after carotid endarterectomy compared to the control group. Symptomatic patients in Group 1 exhibited lower tissue concentration of MMP-8 in comparison to the control group (143.89 pg/ml and 1317.36 respectively; P=0.003). There was a correlation between preoperative MMP-9 levels and tissue concentrations of MMP-8 (P=0.042) and MMP-9 (P=0.019) between symptomatic patients in the control group.

Conclusion: Hydrocortisone reduces the concentration of MMP- 8 in carotid plaque, especially in symptomatic patients. There was an association between systemic and tissue inflammation.

No MeSH data available.


Related in: MedlinePlus

Inflammatory activity of MMP-9 between control group and hydrocortisonegroup.MMP=metalloproteinase; CEA=carotid endarterectomy
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f02: Inflammatory activity of MMP-9 between control group and hydrocortisonegroup.MMP=metalloproteinase; CEA=carotid endarterectomy

Mentions: In the hydrocortisone group, a significant increase in serum levels of MMP-8 (994.28pg/ml and 408.54 pg/ml, respectively; P=0.045) and MMP-9 (106,656.34and 42,807.69 respectively; P=0.014) were identified at 1 hour afterCEA, while lower concentrations of MMP-8 and MMP-9 were observed at 6 hours afterCEA, compared to the control group (Figures 1and 2).


Hydrocortisone supresses inflammatory activity of metalloproteinase-8 in carotid plaque.

Gabriel SA, Antonangelo L, Capelozzi VL, Beteli CB, Camargo Júnior Od, Aquino JL, Caffaro RA - Rev Bras Cir Cardiovasc (2015 Jul-Sep)

Inflammatory activity of MMP-9 between control group and hydrocortisonegroup.MMP=metalloproteinase; CEA=carotid endarterectomy
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4541775&req=5

f02: Inflammatory activity of MMP-9 between control group and hydrocortisonegroup.MMP=metalloproteinase; CEA=carotid endarterectomy
Mentions: In the hydrocortisone group, a significant increase in serum levels of MMP-8 (994.28pg/ml and 408.54 pg/ml, respectively; P=0.045) and MMP-9 (106,656.34and 42,807.69 respectively; P=0.014) were identified at 1 hour afterCEA, while lower concentrations of MMP-8 and MMP-9 were observed at 6 hours afterCEA, compared to the control group (Figures 1and 2).

Bottom Line: Symptomatic patients in Group 1 exhibited lower tissue concentration of MMP-8 in comparison to the control group (143.89 pg/ml and 1317.36 respectively; P=0.003).There was a correlation between preoperative MMP-9 levels and tissue concentrations of MMP-8 (P=0.042) and MMP-9 (P=0.019) between symptomatic patients in the control group.There was an association between systemic and tissue inflammation.

View Article: PubMed Central - PubMed

Affiliation: Faculdade de Medicina, Pontifícia Universidade Católica de Campinas, Campinas, SP, BR.

ABSTRACT

Objective: Matrix metalloproteinases are inflammatory biomarkers involved in carotid plaque instability. Our objective was to analyze the inflammatory activity of plasma and carotid plaque MMP-8 and MMP-9 after intravenous administration of hydrocortisone.

Methods: The study included 22 patients with stenosis ≥ 70% in the carotid artery (11 symptomatic and 11 asymptomatic) who underwent carotid endarterectomy. The patients were divided into two groups: Control Group - hydrocortisone was not administered, and Group 1 - 500 mg intravenous hydrocortisone was administered during anesthetic induction. Plasma levels of MMP-8 and MMP-9 were measured preoperatively (24 hours before carotid endarterectomy) and at 1 hour, 6 hours and 24 hours after carotid endarterectomy. In carotid plaque, tissue levels of MMP-8 and MMP-9 were measured.

Results: Group 1 showed increased serum levels of MMP- 8 (994.28 pg/ml and 408.54 pg/ml, respectively; P=0.045) and MMP-9 (106,656.34 and 42,807.69 respectively; P=0.014) at 1 hour after carotid endarterectomy compared to the control group. Symptomatic patients in Group 1 exhibited lower tissue concentration of MMP-8 in comparison to the control group (143.89 pg/ml and 1317.36 respectively; P=0.003). There was a correlation between preoperative MMP-9 levels and tissue concentrations of MMP-8 (P=0.042) and MMP-9 (P=0.019) between symptomatic patients in the control group.

Conclusion: Hydrocortisone reduces the concentration of MMP- 8 in carotid plaque, especially in symptomatic patients. There was an association between systemic and tissue inflammation.

No MeSH data available.


Related in: MedlinePlus