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Low CD1c + myeloid dendritic cell counts correlated with a high risk of rapid disease progression during early HIV-1 infection.

Diao Y, Geng W, Fan X, Cui H, Sun H, Jiang Y, Wang Y, Sun A, Shang H - BMC Infect. Dis. (2015)

Bottom Line: When compared with the normal controls, patients with EHI displayed significantly lower CD1c + mDC counts and IL-12 secretion and increased surface markers.CD1c + mDC counts were positively correlated with CD4+ T cell counts and inversely associated with viral loads.IL-12 secretion was only positively associated with CD4+ T cell counts.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning Province, China. cmudyy@163.com.

ABSTRACT

Background: During early HIV-1 infection (EHI), the interaction between the immune response and the virus determines disease progression. Although CD1c + myeloid dendritic cells (mDCs) can trigger the immune response, the relationship between CD1c + mDC alteration and disease progression has not yet been defined.

Methods: EHI changes in CD1c + mDC counts, surface marker (CD40, CD86, CD83) expression, and IL-12 secretion were assessed by flow cytometry in 29 patients.

Results: When compared with the normal controls, patients with EHI displayed significantly lower CD1c + mDC counts and IL-12 secretion and increased surface markers. CD1c + mDC counts were positively correlated with CD4+ T cell counts and inversely associated with viral loads. IL-12 secretion was only positively associated with CD4+ T cell counts. Rapid progressors had lower counts, CD86 expression, and IL-12 secretion of CD1c + mDCs comparing with typical progressors. Kaplan-Meier analysis and Cox regression models suggested patients with low CD1c + mDC counts (<10 cells/μL) had a 4-fold higher risk of rapid disease progression than those with high CD1c + mDC counts. However, no relationship was found between surface markers or IL-12 secretion and disease progression.

Conclusions: During EHI, patients with low CD1c + mDC counts were more likely to experience rapid disease progression than those with high CD1c + mDC counts.

No MeSH data available.


Related in: MedlinePlus

Abnormally low CD1c + mDC counts, CD86 expression, and IL-12 secretion in RPs compared with TPs. a Flow cytometry plots of CD1c + mDC counts, CD86 expression, and IL-12 secretion in RPs. Light gray histograms represented isotype control staining for CD86 expression or IL-12 secretion. b Flow cytometry plots of CD1c + mDC counts, CD86 expression, and IL-12 secretion in TPs. Light gray histograms represented isotype control staining for CD86 expression or IL-12 secretion. c CD1c + mDC counts in RPs and in TPs. d Expression of CD86 on CD1c + mDC in RPs compared with TPs. e Intracellular secretion of IL-12 in CD1c + mDCs from RPs and TPs after stimulation with R848
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Fig2: Abnormally low CD1c + mDC counts, CD86 expression, and IL-12 secretion in RPs compared with TPs. a Flow cytometry plots of CD1c + mDC counts, CD86 expression, and IL-12 secretion in RPs. Light gray histograms represented isotype control staining for CD86 expression or IL-12 secretion. b Flow cytometry plots of CD1c + mDC counts, CD86 expression, and IL-12 secretion in TPs. Light gray histograms represented isotype control staining for CD86 expression or IL-12 secretion. c CD1c + mDC counts in RPs and in TPs. d Expression of CD86 on CD1c + mDC in RPs compared with TPs. e Intracellular secretion of IL-12 in CD1c + mDCs from RPs and TPs after stimulation with R848

Mentions: Since the disease in RPs progressed more rapidly, we next determined whether abnormally low CD1c + mDC counts and expression of CD40, CD86, and CD83, and IL-12 secretion would be evident in RPs. CD1c + mDC counts in RPs were significantly lower than those in TPs (p = 0.018) (Fig. 2a, 2b, 2c). In addition to CD40 and CD83, RPs also had lower CD86 expression than TPs (p = 0.045) (Fig. 2a, 2b, 2d). CD1c + mDC secretion of IL-12 in RPs was also significantly lower than TPs stimulated with R848 (p = 0.004) (Fig. 2a, 2b, 2e).Fig. 2


Low CD1c + myeloid dendritic cell counts correlated with a high risk of rapid disease progression during early HIV-1 infection.

Diao Y, Geng W, Fan X, Cui H, Sun H, Jiang Y, Wang Y, Sun A, Shang H - BMC Infect. Dis. (2015)

Abnormally low CD1c + mDC counts, CD86 expression, and IL-12 secretion in RPs compared with TPs. a Flow cytometry plots of CD1c + mDC counts, CD86 expression, and IL-12 secretion in RPs. Light gray histograms represented isotype control staining for CD86 expression or IL-12 secretion. b Flow cytometry plots of CD1c + mDC counts, CD86 expression, and IL-12 secretion in TPs. Light gray histograms represented isotype control staining for CD86 expression or IL-12 secretion. c CD1c + mDC counts in RPs and in TPs. d Expression of CD86 on CD1c + mDC in RPs compared with TPs. e Intracellular secretion of IL-12 in CD1c + mDCs from RPs and TPs after stimulation with R848
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4541738&req=5

Fig2: Abnormally low CD1c + mDC counts, CD86 expression, and IL-12 secretion in RPs compared with TPs. a Flow cytometry plots of CD1c + mDC counts, CD86 expression, and IL-12 secretion in RPs. Light gray histograms represented isotype control staining for CD86 expression or IL-12 secretion. b Flow cytometry plots of CD1c + mDC counts, CD86 expression, and IL-12 secretion in TPs. Light gray histograms represented isotype control staining for CD86 expression or IL-12 secretion. c CD1c + mDC counts in RPs and in TPs. d Expression of CD86 on CD1c + mDC in RPs compared with TPs. e Intracellular secretion of IL-12 in CD1c + mDCs from RPs and TPs after stimulation with R848
Mentions: Since the disease in RPs progressed more rapidly, we next determined whether abnormally low CD1c + mDC counts and expression of CD40, CD86, and CD83, and IL-12 secretion would be evident in RPs. CD1c + mDC counts in RPs were significantly lower than those in TPs (p = 0.018) (Fig. 2a, 2b, 2c). In addition to CD40 and CD83, RPs also had lower CD86 expression than TPs (p = 0.045) (Fig. 2a, 2b, 2d). CD1c + mDC secretion of IL-12 in RPs was also significantly lower than TPs stimulated with R848 (p = 0.004) (Fig. 2a, 2b, 2e).Fig. 2

Bottom Line: When compared with the normal controls, patients with EHI displayed significantly lower CD1c + mDC counts and IL-12 secretion and increased surface markers.CD1c + mDC counts were positively correlated with CD4+ T cell counts and inversely associated with viral loads.IL-12 secretion was only positively associated with CD4+ T cell counts.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning Province, China. cmudyy@163.com.

ABSTRACT

Background: During early HIV-1 infection (EHI), the interaction between the immune response and the virus determines disease progression. Although CD1c + myeloid dendritic cells (mDCs) can trigger the immune response, the relationship between CD1c + mDC alteration and disease progression has not yet been defined.

Methods: EHI changes in CD1c + mDC counts, surface marker (CD40, CD86, CD83) expression, and IL-12 secretion were assessed by flow cytometry in 29 patients.

Results: When compared with the normal controls, patients with EHI displayed significantly lower CD1c + mDC counts and IL-12 secretion and increased surface markers. CD1c + mDC counts were positively correlated with CD4+ T cell counts and inversely associated with viral loads. IL-12 secretion was only positively associated with CD4+ T cell counts. Rapid progressors had lower counts, CD86 expression, and IL-12 secretion of CD1c + mDCs comparing with typical progressors. Kaplan-Meier analysis and Cox regression models suggested patients with low CD1c + mDC counts (<10 cells/μL) had a 4-fold higher risk of rapid disease progression than those with high CD1c + mDC counts. However, no relationship was found between surface markers or IL-12 secretion and disease progression.

Conclusions: During EHI, patients with low CD1c + mDC counts were more likely to experience rapid disease progression than those with high CD1c + mDC counts.

No MeSH data available.


Related in: MedlinePlus