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Transcriptomes analysis of Aeromonas molluscorum Av27 cells exposed to tributyltin (TBT): Unravelling the effects from the molecular level to the organism.

Cruz A, Rodrigues R, Pinheiro M, Mendo S - Mar. Environ. Res. (2015)

Bottom Line: At both TBT concentrations, higher variations in gene expression were found in the functional categories associated with enzymatic activities, transport/binding and oxidation-reduction.Furthermore it contributes to the knowledge of the functional genomic aspects of bacteria exposed to this pollutant.Furthermore, the transcriptomic data gathered, and now publically available, constitute a valuable resource for comparative genome analysis.

View Article: PubMed Central - PubMed

Affiliation: Biology Department & CESAM, University of Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal. Electronic address: andreia.cruz@ua.pt.

No MeSH data available.


Related in: MedlinePlus

Functional categories of A. molluscorum Av27 over and under-expressed genes, following exposure to 5 μM (A) and 50 μM of TBT (B).
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fig1: Functional categories of A. molluscorum Av27 over and under-expressed genes, following exposure to 5 μM (A) and 50 μM of TBT (B).

Mentions: To further investigate specific processes, functions or cellular structures affected by TBT, GO annotations were performed. The information retrieved regarding GO associated with Biological Process, Cellular Component and Molecular Function identified in each condition (control, 5 μM and 50 μM TBT) is shown in Figure S1 (Supporting Information). In general, regarding Biological Processes, all of the tested conditions revealed most of the sequences assigning with “Metabolic Process” and “Cellular Process”. Furthermore, the matches of Cellular Component terms were prevalent within the “Cell”, “Cell Part” followed by the “Macromolecular Complex” category. Finally, for the Molecular Function GO the most dominant matches were found with the “Catalytic Activity” and “Binding” categories. The similarity of the results obtained between all the samples makes it difficult to draw conclusions. Thus, the number of over and under-expressed genes on each category was analysed, in comparison to the control samples (Fig. 1). This analysis revealed that exposure to 5 μM TBT results in a higher number of repressed genes in comparison with over-expressed ones, which could be related with energy saving and/or survival of the cell. Similarly, also to save energy, a repression of several genes was observed in Lactococcus lactis after infection by a bacteriophage (Fallico et al., 2011). Moreover, this result may also be related with the fact that fewer reads were obtained in the library corresponding to the cells exposed to 5 μM in comparison with the control library. At 50 μM of TBT, the number of over-expressed genes is higher than the repressed ones, which might be related with the activation of the defence mechanisms to withstand the stressful concentration. At both TBT concentrations, higher variation in both the over and under-expressed genes was found in the enzymatic activity functional category, followed by transport/binding and oxidation-reduction. These categories included genes that are mainly over-expressed at 50 μM (Table 1), suggesting their possible involvement to protect against TBT. It has been suggested that TBT can be exported from the cell through efflux pumps (Jude et al., 2004) and/or it can be degraded through dealkylation and demethylation (Barug, 1981), involving enzymes encoded by genes belonging to the above referred functional categories.


Transcriptomes analysis of Aeromonas molluscorum Av27 cells exposed to tributyltin (TBT): Unravelling the effects from the molecular level to the organism.

Cruz A, Rodrigues R, Pinheiro M, Mendo S - Mar. Environ. Res. (2015)

Functional categories of A. molluscorum Av27 over and under-expressed genes, following exposure to 5 μM (A) and 50 μM of TBT (B).
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4541717&req=5

fig1: Functional categories of A. molluscorum Av27 over and under-expressed genes, following exposure to 5 μM (A) and 50 μM of TBT (B).
Mentions: To further investigate specific processes, functions or cellular structures affected by TBT, GO annotations were performed. The information retrieved regarding GO associated with Biological Process, Cellular Component and Molecular Function identified in each condition (control, 5 μM and 50 μM TBT) is shown in Figure S1 (Supporting Information). In general, regarding Biological Processes, all of the tested conditions revealed most of the sequences assigning with “Metabolic Process” and “Cellular Process”. Furthermore, the matches of Cellular Component terms were prevalent within the “Cell”, “Cell Part” followed by the “Macromolecular Complex” category. Finally, for the Molecular Function GO the most dominant matches were found with the “Catalytic Activity” and “Binding” categories. The similarity of the results obtained between all the samples makes it difficult to draw conclusions. Thus, the number of over and under-expressed genes on each category was analysed, in comparison to the control samples (Fig. 1). This analysis revealed that exposure to 5 μM TBT results in a higher number of repressed genes in comparison with over-expressed ones, which could be related with energy saving and/or survival of the cell. Similarly, also to save energy, a repression of several genes was observed in Lactococcus lactis after infection by a bacteriophage (Fallico et al., 2011). Moreover, this result may also be related with the fact that fewer reads were obtained in the library corresponding to the cells exposed to 5 μM in comparison with the control library. At 50 μM of TBT, the number of over-expressed genes is higher than the repressed ones, which might be related with the activation of the defence mechanisms to withstand the stressful concentration. At both TBT concentrations, higher variation in both the over and under-expressed genes was found in the enzymatic activity functional category, followed by transport/binding and oxidation-reduction. These categories included genes that are mainly over-expressed at 50 μM (Table 1), suggesting their possible involvement to protect against TBT. It has been suggested that TBT can be exported from the cell through efflux pumps (Jude et al., 2004) and/or it can be degraded through dealkylation and demethylation (Barug, 1981), involving enzymes encoded by genes belonging to the above referred functional categories.

Bottom Line: At both TBT concentrations, higher variations in gene expression were found in the functional categories associated with enzymatic activities, transport/binding and oxidation-reduction.Furthermore it contributes to the knowledge of the functional genomic aspects of bacteria exposed to this pollutant.Furthermore, the transcriptomic data gathered, and now publically available, constitute a valuable resource for comparative genome analysis.

View Article: PubMed Central - PubMed

Affiliation: Biology Department & CESAM, University of Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal. Electronic address: andreia.cruz@ua.pt.

No MeSH data available.


Related in: MedlinePlus