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Changes in expression of BDNF and its receptors TrkB and p75NTR in the hippocampus of a dog model of chronic alcoholism and abstinence.

Xu R, Duan SR, Zhao JW, Wang CY - Braz. J. Med. Biol. Res. (2015)

Bottom Line: BDNF, TrkB and p75NTR-positive cells were mainly localized in the granular cell layer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4 regions (DG>CA1>CA3>CA4).The CA4 region appeared to show the greatest differences.Changes in p75NTR expression were the opposite of those of BDNF and TrkB, with the greatest differences observed in the DG and CA4 regions.

View Article: PubMed Central - PubMed

Affiliation: Neurology Ward of Internal Medicine, Hospital of Harbin Medical University, Heilongjiang Province, Harbin, China.

ABSTRACT
Chronic ethanol consumption can produce learning and memory deficits. Brain-derived neurotrophic factor (BDNF) and its receptors affect the pathogenesis of alcoholism. In this study, we examined the expression of BDNF, tropomyosin receptor kinase B (TrkB) and p75 neurotrophin receptor (p75NTR) in the hippocampus of a dog model of chronic alcoholism and abstinence. Twenty domestic dogs (9-10 months old, 15-20 kg; 10 males and 10 females) were obtained from Harbin Medical University. A stable alcoholism model was established through ad libitum feeding, and anti-alcohol drug treatment (Zhong Yao Jie Jiu Ling, the main ingredient was the stems of watermelon; developed in our laboratory), at low- and high-doses, was carried out. The Zhong Yao Jie Jiu Ling was effective for the alcoholism in dogs. The morphology of hippocampal neurons was evaluated using hematoxylin-eosin staining. The number and morphological features of BDNF, TrkB and p75NTR-positive neurons in the dentate gyrus (DG), and the CA1, CA3 and CA4 regions of the hippocampus were observed using immunohistochemistry. One-way ANOVA was used to determine differences in BDNF, TrkB and p75NTR expression. BDNF, TrkB and p75NTR-positive cells were mainly localized in the granular cell layer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4 regions (DG>CA1>CA3>CA4). Expression levels of both BDNF and TrkB were decreased in chronic alcoholism, and increased after abstinence. The CA4 region appeared to show the greatest differences. Changes in p75NTR expression were the opposite of those of BDNF and TrkB, with the greatest differences observed in the DG and CA4 regions.

No MeSH data available.


Related in: MedlinePlus

p75 neurotrophin receptor (p75NTR)-positive neurons in the dentate gyrus(DG) and hippocampus (CA4, CA3, and CA1) in the four groups (bars: 100 μm). Thearrows indicate p75NTR-positive neurons.
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f04: p75 neurotrophin receptor (p75NTR)-positive neurons in the dentate gyrus(DG) and hippocampus (CA4, CA3, and CA1) in the four groups (bars: 100 μm). Thearrows indicate p75NTR-positive neurons.

Mentions: In the hippocampus, p75NTR-positive cells were mainly concentrated in the granularcell layer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4 regions(Figure 4). The number of positive neuronsin these regions decreased in the following order: DG, CA1, CA3 and CA4. Comparedwith the control group, the number of p75NTR-positive cells in each of these regionswas significantly increased in the alcoholism group (P<0.05). Compared with thealcoholism group, the number of p75NTR-positive cells in each of these regions wassignificantly decreased in the high-dose drug treatment group (P<0.05). Inaddition, compared with the alcoholism group, the numbers of p75NTR-positive cells inthe DG and the CA4 region in the low-dose drug treatment group were alsosignificantly decreased (P<0.05). Furthermore, compared with the alcoholism group,the number of p75NTR-positive cells in the low- and high-dose drug treatment groupswere decreased, and there was no statistically significant difference from thecontrol group (P>0.05; Table 3).


Changes in expression of BDNF and its receptors TrkB and p75NTR in the hippocampus of a dog model of chronic alcoholism and abstinence.

Xu R, Duan SR, Zhao JW, Wang CY - Braz. J. Med. Biol. Res. (2015)

p75 neurotrophin receptor (p75NTR)-positive neurons in the dentate gyrus(DG) and hippocampus (CA4, CA3, and CA1) in the four groups (bars: 100 μm). Thearrows indicate p75NTR-positive neurons.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4541689&req=5

f04: p75 neurotrophin receptor (p75NTR)-positive neurons in the dentate gyrus(DG) and hippocampus (CA4, CA3, and CA1) in the four groups (bars: 100 μm). Thearrows indicate p75NTR-positive neurons.
Mentions: In the hippocampus, p75NTR-positive cells were mainly concentrated in the granularcell layer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4 regions(Figure 4). The number of positive neuronsin these regions decreased in the following order: DG, CA1, CA3 and CA4. Comparedwith the control group, the number of p75NTR-positive cells in each of these regionswas significantly increased in the alcoholism group (P<0.05). Compared with thealcoholism group, the number of p75NTR-positive cells in each of these regions wassignificantly decreased in the high-dose drug treatment group (P<0.05). Inaddition, compared with the alcoholism group, the numbers of p75NTR-positive cells inthe DG and the CA4 region in the low-dose drug treatment group were alsosignificantly decreased (P<0.05). Furthermore, compared with the alcoholism group,the number of p75NTR-positive cells in the low- and high-dose drug treatment groupswere decreased, and there was no statistically significant difference from thecontrol group (P>0.05; Table 3).

Bottom Line: BDNF, TrkB and p75NTR-positive cells were mainly localized in the granular cell layer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4 regions (DG>CA1>CA3>CA4).The CA4 region appeared to show the greatest differences.Changes in p75NTR expression were the opposite of those of BDNF and TrkB, with the greatest differences observed in the DG and CA4 regions.

View Article: PubMed Central - PubMed

Affiliation: Neurology Ward of Internal Medicine, Hospital of Harbin Medical University, Heilongjiang Province, Harbin, China.

ABSTRACT
Chronic ethanol consumption can produce learning and memory deficits. Brain-derived neurotrophic factor (BDNF) and its receptors affect the pathogenesis of alcoholism. In this study, we examined the expression of BDNF, tropomyosin receptor kinase B (TrkB) and p75 neurotrophin receptor (p75NTR) in the hippocampus of a dog model of chronic alcoholism and abstinence. Twenty domestic dogs (9-10 months old, 15-20 kg; 10 males and 10 females) were obtained from Harbin Medical University. A stable alcoholism model was established through ad libitum feeding, and anti-alcohol drug treatment (Zhong Yao Jie Jiu Ling, the main ingredient was the stems of watermelon; developed in our laboratory), at low- and high-doses, was carried out. The Zhong Yao Jie Jiu Ling was effective for the alcoholism in dogs. The morphology of hippocampal neurons was evaluated using hematoxylin-eosin staining. The number and morphological features of BDNF, TrkB and p75NTR-positive neurons in the dentate gyrus (DG), and the CA1, CA3 and CA4 regions of the hippocampus were observed using immunohistochemistry. One-way ANOVA was used to determine differences in BDNF, TrkB and p75NTR expression. BDNF, TrkB and p75NTR-positive cells were mainly localized in the granular cell layer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4 regions (DG>CA1>CA3>CA4). Expression levels of both BDNF and TrkB were decreased in chronic alcoholism, and increased after abstinence. The CA4 region appeared to show the greatest differences. Changes in p75NTR expression were the opposite of those of BDNF and TrkB, with the greatest differences observed in the DG and CA4 regions.

No MeSH data available.


Related in: MedlinePlus