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Changes in expression of BDNF and its receptors TrkB and p75NTR in the hippocampus of a dog model of chronic alcoholism and abstinence.

Xu R, Duan SR, Zhao JW, Wang CY - Braz. J. Med. Biol. Res. (2015)

Bottom Line: BDNF, TrkB and p75NTR-positive cells were mainly localized in the granular cell layer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4 regions (DG>CA1>CA3>CA4).The CA4 region appeared to show the greatest differences.Changes in p75NTR expression were the opposite of those of BDNF and TrkB, with the greatest differences observed in the DG and CA4 regions.

View Article: PubMed Central - PubMed

Affiliation: Neurology Ward of Internal Medicine, Hospital of Harbin Medical University, Heilongjiang Province, Harbin, China.

ABSTRACT
Chronic ethanol consumption can produce learning and memory deficits. Brain-derived neurotrophic factor (BDNF) and its receptors affect the pathogenesis of alcoholism. In this study, we examined the expression of BDNF, tropomyosin receptor kinase B (TrkB) and p75 neurotrophin receptor (p75NTR) in the hippocampus of a dog model of chronic alcoholism and abstinence. Twenty domestic dogs (9-10 months old, 15-20 kg; 10 males and 10 females) were obtained from Harbin Medical University. A stable alcoholism model was established through ad libitum feeding, and anti-alcohol drug treatment (Zhong Yao Jie Jiu Ling, the main ingredient was the stems of watermelon; developed in our laboratory), at low- and high-doses, was carried out. The Zhong Yao Jie Jiu Ling was effective for the alcoholism in dogs. The morphology of hippocampal neurons was evaluated using hematoxylin-eosin staining. The number and morphological features of BDNF, TrkB and p75NTR-positive neurons in the dentate gyrus (DG), and the CA1, CA3 and CA4 regions of the hippocampus were observed using immunohistochemistry. One-way ANOVA was used to determine differences in BDNF, TrkB and p75NTR expression. BDNF, TrkB and p75NTR-positive cells were mainly localized in the granular cell layer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4 regions (DG>CA1>CA3>CA4). Expression levels of both BDNF and TrkB were decreased in chronic alcoholism, and increased after abstinence. The CA4 region appeared to show the greatest differences. Changes in p75NTR expression were the opposite of those of BDNF and TrkB, with the greatest differences observed in the DG and CA4 regions.

No MeSH data available.


Related in: MedlinePlus

Brain-derived neurotrophic factor (BDNF)-positive neurons in the dentategyrus (DG) and hippocampus (CA4, CA3, and CA1) in the four groups (bars: 100mm). The arrows indicate BDNF-positive neurons.
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f02: Brain-derived neurotrophic factor (BDNF)-positive neurons in the dentategyrus (DG) and hippocampus (CA4, CA3, and CA1) in the four groups (bars: 100mm). The arrows indicate BDNF-positive neurons.

Mentions: In the hippocampus, BDNF-positive cells were mainly concentrated in the granular celllayer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4 regions(Figure 2). The number of BDNF-positiveneurons in the different regions decreased in the following order: DG, CA1, CA3 andCA4. Compared with the control group, the number of BDNF-positive cells in thegranular cell layer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4regions were significantly decreased in the alcoholism group (P<0.05). Comparedwith the alcoholism group, the numbers of BDNF-positive cells in the granular celllayer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4 regions weresignificantly increased in the high-dose drug treatment group (P<0.05).Furthermore, compared with the alcoholism group, the numbers of BDNF-positive cellsin the granular cell layer of the DG and in the pyramidal cell layer of the CA1, CA3and CA4 regions were increased in the high-dose drug treatment group, but only theCA4 region showed a statistically significant difference (P<0.05). Moreover, theDG, and the CA1, CA2, CA3 and CA4 regions displayed significant differences betweenthe high-dose drug treatment and control groups (P<0.05). Only the CA4 region inthe low-dose drug treatment group showed a statistically significant difference fromcontrol (P<0.05; Table 1).


Changes in expression of BDNF and its receptors TrkB and p75NTR in the hippocampus of a dog model of chronic alcoholism and abstinence.

Xu R, Duan SR, Zhao JW, Wang CY - Braz. J. Med. Biol. Res. (2015)

Brain-derived neurotrophic factor (BDNF)-positive neurons in the dentategyrus (DG) and hippocampus (CA4, CA3, and CA1) in the four groups (bars: 100mm). The arrows indicate BDNF-positive neurons.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4541689&req=5

f02: Brain-derived neurotrophic factor (BDNF)-positive neurons in the dentategyrus (DG) and hippocampus (CA4, CA3, and CA1) in the four groups (bars: 100mm). The arrows indicate BDNF-positive neurons.
Mentions: In the hippocampus, BDNF-positive cells were mainly concentrated in the granular celllayer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4 regions(Figure 2). The number of BDNF-positiveneurons in the different regions decreased in the following order: DG, CA1, CA3 andCA4. Compared with the control group, the number of BDNF-positive cells in thegranular cell layer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4regions were significantly decreased in the alcoholism group (P<0.05). Comparedwith the alcoholism group, the numbers of BDNF-positive cells in the granular celllayer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4 regions weresignificantly increased in the high-dose drug treatment group (P<0.05).Furthermore, compared with the alcoholism group, the numbers of BDNF-positive cellsin the granular cell layer of the DG and in the pyramidal cell layer of the CA1, CA3and CA4 regions were increased in the high-dose drug treatment group, but only theCA4 region showed a statistically significant difference (P<0.05). Moreover, theDG, and the CA1, CA2, CA3 and CA4 regions displayed significant differences betweenthe high-dose drug treatment and control groups (P<0.05). Only the CA4 region inthe low-dose drug treatment group showed a statistically significant difference fromcontrol (P<0.05; Table 1).

Bottom Line: BDNF, TrkB and p75NTR-positive cells were mainly localized in the granular cell layer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4 regions (DG>CA1>CA3>CA4).The CA4 region appeared to show the greatest differences.Changes in p75NTR expression were the opposite of those of BDNF and TrkB, with the greatest differences observed in the DG and CA4 regions.

View Article: PubMed Central - PubMed

Affiliation: Neurology Ward of Internal Medicine, Hospital of Harbin Medical University, Heilongjiang Province, Harbin, China.

ABSTRACT
Chronic ethanol consumption can produce learning and memory deficits. Brain-derived neurotrophic factor (BDNF) and its receptors affect the pathogenesis of alcoholism. In this study, we examined the expression of BDNF, tropomyosin receptor kinase B (TrkB) and p75 neurotrophin receptor (p75NTR) in the hippocampus of a dog model of chronic alcoholism and abstinence. Twenty domestic dogs (9-10 months old, 15-20 kg; 10 males and 10 females) were obtained from Harbin Medical University. A stable alcoholism model was established through ad libitum feeding, and anti-alcohol drug treatment (Zhong Yao Jie Jiu Ling, the main ingredient was the stems of watermelon; developed in our laboratory), at low- and high-doses, was carried out. The Zhong Yao Jie Jiu Ling was effective for the alcoholism in dogs. The morphology of hippocampal neurons was evaluated using hematoxylin-eosin staining. The number and morphological features of BDNF, TrkB and p75NTR-positive neurons in the dentate gyrus (DG), and the CA1, CA3 and CA4 regions of the hippocampus were observed using immunohistochemistry. One-way ANOVA was used to determine differences in BDNF, TrkB and p75NTR expression. BDNF, TrkB and p75NTR-positive cells were mainly localized in the granular cell layer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4 regions (DG>CA1>CA3>CA4). Expression levels of both BDNF and TrkB were decreased in chronic alcoholism, and increased after abstinence. The CA4 region appeared to show the greatest differences. Changes in p75NTR expression were the opposite of those of BDNF and TrkB, with the greatest differences observed in the DG and CA4 regions.

No MeSH data available.


Related in: MedlinePlus