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Rat visceral yolk sac cells: viability and expression of cell markers during maternal diabetes.

Aires MB, Santos JR, Souza KS, Farias PS, Santos AC, Fioretto ET, Maria DA - Braz. J. Med. Biol. Res. (2015)

Bottom Line: Fetal weight was reduced in the diabetic group.In the diabetic group, significantly decreased expression of CD34 (P<0.05), CCR2 (P<0.001), and OCT3/4 (P<0.01), and significantly increased expression of CD90 (P<0.05), CD117 (P<0.01), and CD14 (P<0.05) were observed.Severe hyperglycemia caused by maternal diabetes had negative effects on pregnancy, VYS cell viability, and the expression of cell markers.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Morfologia, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.

ABSTRACT
The function of the visceral yolk sac (VYS) is critical for embryo organogenesis until final fetal development in rats, and can be affected by conditions such as diabetes. In view of the importance of diabetes during pregnancy for maternal and neonatal health, the objective of this study was to assess fetal weight, VYS cell markers, and viability in female Wistar rats (200-250 g) with induced diabetes (alloxan, 37 mg/kg) on the 8th gestational day (gd 8). At gd 15, rats from control (n=5) and diabetic (n=5) groups were anesthetized and laparotomized to remove the uterine horns for weighing of fetuses and collecting the VYS. Flow cytometry was used for characterizing VYS cells, and for determining mitochondrial activity, cell proliferation, DNA ploidy, cell cycle phases, and caspase-3 activity. Fetal weight was reduced in the diabetic group. Expression of the cell markers CD34, VEGFR1, CD115, CD117, CD14, CCR2, CD90, CD44, STRO-1, OCT3/4, and Nanog was detected in VYS cells in both groups. In the diabetic group, significantly decreased expression of CD34 (P<0.05), CCR2 (P<0.001), and OCT3/4 (P<0.01), and significantly increased expression of CD90 (P<0.05), CD117 (P<0.01), and CD14 (P<0.05) were observed. VYS cells with inactive mitochondria, activated caspase-3, and low proliferation were present in the rats with diabetes. Severe hyperglycemia caused by maternal diabetes had negative effects on pregnancy, VYS cell viability, and the expression of cell markers.

No MeSH data available.


Related in: MedlinePlus

A, Cell cycle analysis showed the same variable distributionof visceral yolk sac (VYS) cells in all phases for both groups, except for thegreater number of sub-haploid cells in the diabetic group. B,VYS cells showed a lower proliferation index in the diabetic group than in thecontrol group. Results are reported as the means±SE of cells collected fromfive yolk sacs per animal of the control (n=5) and diabetic groups (n=5)analyzed in duplicate. *P<0.05 (Student’s t-test).
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f03: A, Cell cycle analysis showed the same variable distributionof visceral yolk sac (VYS) cells in all phases for both groups, except for thegreater number of sub-haploid cells in the diabetic group. B,VYS cells showed a lower proliferation index in the diabetic group than in thecontrol group. Results are reported as the means±SE of cells collected fromfive yolk sacs per animal of the control (n=5) and diabetic groups (n=5)analyzed in duplicate. *P<0.05 (Student’s t-test).

Mentions: The number of diploid VYS cells was not different at gd 15 in the diabetic group(51.3±6.3) compared with the controls (62.9±5.9). However, fewer cells in the controlgroup were aneuploid (38.4±5.7 vs 62.9±5.0, P<0.05). The resultsof cell cycle analysis showed the distribution of VYS cells in all phases to besimilar for both groups, except for a greater number of sub-haploid cells in thediabetic group (20.2±4.1 vs 10.3±1.9, P<0.05) (Figure 3A). VYS cells showed a low proliferationindex at gd 15 (8.43±1.3, P<0.01) in the diabetic group compared with the controlgroup (21.5±1.8) (Figure 3B).


Rat visceral yolk sac cells: viability and expression of cell markers during maternal diabetes.

Aires MB, Santos JR, Souza KS, Farias PS, Santos AC, Fioretto ET, Maria DA - Braz. J. Med. Biol. Res. (2015)

A, Cell cycle analysis showed the same variable distributionof visceral yolk sac (VYS) cells in all phases for both groups, except for thegreater number of sub-haploid cells in the diabetic group. B,VYS cells showed a lower proliferation index in the diabetic group than in thecontrol group. Results are reported as the means±SE of cells collected fromfive yolk sacs per animal of the control (n=5) and diabetic groups (n=5)analyzed in duplicate. *P<0.05 (Student’s t-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4541685&req=5

f03: A, Cell cycle analysis showed the same variable distributionof visceral yolk sac (VYS) cells in all phases for both groups, except for thegreater number of sub-haploid cells in the diabetic group. B,VYS cells showed a lower proliferation index in the diabetic group than in thecontrol group. Results are reported as the means±SE of cells collected fromfive yolk sacs per animal of the control (n=5) and diabetic groups (n=5)analyzed in duplicate. *P<0.05 (Student’s t-test).
Mentions: The number of diploid VYS cells was not different at gd 15 in the diabetic group(51.3±6.3) compared with the controls (62.9±5.9). However, fewer cells in the controlgroup were aneuploid (38.4±5.7 vs 62.9±5.0, P<0.05). The resultsof cell cycle analysis showed the distribution of VYS cells in all phases to besimilar for both groups, except for a greater number of sub-haploid cells in thediabetic group (20.2±4.1 vs 10.3±1.9, P<0.05) (Figure 3A). VYS cells showed a low proliferationindex at gd 15 (8.43±1.3, P<0.01) in the diabetic group compared with the controlgroup (21.5±1.8) (Figure 3B).

Bottom Line: Fetal weight was reduced in the diabetic group.In the diabetic group, significantly decreased expression of CD34 (P<0.05), CCR2 (P<0.001), and OCT3/4 (P<0.01), and significantly increased expression of CD90 (P<0.05), CD117 (P<0.01), and CD14 (P<0.05) were observed.Severe hyperglycemia caused by maternal diabetes had negative effects on pregnancy, VYS cell viability, and the expression of cell markers.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Morfologia, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.

ABSTRACT
The function of the visceral yolk sac (VYS) is critical for embryo organogenesis until final fetal development in rats, and can be affected by conditions such as diabetes. In view of the importance of diabetes during pregnancy for maternal and neonatal health, the objective of this study was to assess fetal weight, VYS cell markers, and viability in female Wistar rats (200-250 g) with induced diabetes (alloxan, 37 mg/kg) on the 8th gestational day (gd 8). At gd 15, rats from control (n=5) and diabetic (n=5) groups were anesthetized and laparotomized to remove the uterine horns for weighing of fetuses and collecting the VYS. Flow cytometry was used for characterizing VYS cells, and for determining mitochondrial activity, cell proliferation, DNA ploidy, cell cycle phases, and caspase-3 activity. Fetal weight was reduced in the diabetic group. Expression of the cell markers CD34, VEGFR1, CD115, CD117, CD14, CCR2, CD90, CD44, STRO-1, OCT3/4, and Nanog was detected in VYS cells in both groups. In the diabetic group, significantly decreased expression of CD34 (P<0.05), CCR2 (P<0.001), and OCT3/4 (P<0.01), and significantly increased expression of CD90 (P<0.05), CD117 (P<0.01), and CD14 (P<0.05) were observed. VYS cells with inactive mitochondria, activated caspase-3, and low proliferation were present in the rats with diabetes. Severe hyperglycemia caused by maternal diabetes had negative effects on pregnancy, VYS cell viability, and the expression of cell markers.

No MeSH data available.


Related in: MedlinePlus