Limits...
Rat visceral yolk sac cells: viability and expression of cell markers during maternal diabetes.

Aires MB, Santos JR, Souza KS, Farias PS, Santos AC, Fioretto ET, Maria DA - Braz. J. Med. Biol. Res. (2015)

Bottom Line: Fetal weight was reduced in the diabetic group.In the diabetic group, significantly decreased expression of CD34 (P<0.05), CCR2 (P<0.001), and OCT3/4 (P<0.01), and significantly increased expression of CD90 (P<0.05), CD117 (P<0.01), and CD14 (P<0.05) were observed.Severe hyperglycemia caused by maternal diabetes had negative effects on pregnancy, VYS cell viability, and the expression of cell markers.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Morfologia, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.

ABSTRACT
The function of the visceral yolk sac (VYS) is critical for embryo organogenesis until final fetal development in rats, and can be affected by conditions such as diabetes. In view of the importance of diabetes during pregnancy for maternal and neonatal health, the objective of this study was to assess fetal weight, VYS cell markers, and viability in female Wistar rats (200-250 g) with induced diabetes (alloxan, 37 mg/kg) on the 8th gestational day (gd 8). At gd 15, rats from control (n=5) and diabetic (n=5) groups were anesthetized and laparotomized to remove the uterine horns for weighing of fetuses and collecting the VYS. Flow cytometry was used for characterizing VYS cells, and for determining mitochondrial activity, cell proliferation, DNA ploidy, cell cycle phases, and caspase-3 activity. Fetal weight was reduced in the diabetic group. Expression of the cell markers CD34, VEGFR1, CD115, CD117, CD14, CCR2, CD90, CD44, STRO-1, OCT3/4, and Nanog was detected in VYS cells in both groups. In the diabetic group, significantly decreased expression of CD34 (P<0.05), CCR2 (P<0.001), and OCT3/4 (P<0.01), and significantly increased expression of CD90 (P<0.05), CD117 (P<0.01), and CD14 (P<0.05) were observed. VYS cells with inactive mitochondria, activated caspase-3, and low proliferation were present in the rats with diabetes. Severe hyperglycemia caused by maternal diabetes had negative effects on pregnancy, VYS cell viability, and the expression of cell markers.

No MeSH data available.


Related in: MedlinePlus

A, Flow cytometry plots of visceral yolk sac (VYS) cells fromcontrol and diabetic groups stained with rhodamine 123. The histograms show anincreased number of VYS cells with inactive mitochondria (unviable cells) inthe diabetic group and a similar number of cells with active mitochondria(viable cells) in both groups. B, Representative flowcytometry plot of activated caspase-3 in VYS cells of control and diabeticgroups. The histogram shows a large number of activated caspase-3-expressingVYS cells in the diabetic group. Results are reported as the means±SE of VYScells collected from five yolk sacs per animal of the control (n=5) anddiabetic groups (n=5) analyzed in duplicate. *P<0.05 (Student’st-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4541685&req=5

f02: A, Flow cytometry plots of visceral yolk sac (VYS) cells fromcontrol and diabetic groups stained with rhodamine 123. The histograms show anincreased number of VYS cells with inactive mitochondria (unviable cells) inthe diabetic group and a similar number of cells with active mitochondria(viable cells) in both groups. B, Representative flowcytometry plot of activated caspase-3 in VYS cells of control and diabeticgroups. The histogram shows a large number of activated caspase-3-expressingVYS cells in the diabetic group. Results are reported as the means±SE of VYScells collected from five yolk sacs per animal of the control (n=5) anddiabetic groups (n=5) analyzed in duplicate. *P<0.05 (Student’st-test).

Mentions: Cellular uptake of rhodamine 123 was analyzed and compared between groups (Figure 2A). VYS cells from the diabetic groupshowed a similar number of viable cells with active mitochondria (88.14±1.6) comparedwith the control group (94.1±1.3). However, an increased number of VYS cells withinactive mitochondria (nonviable cells) were present in the diabetic group (12.4±1.5vs 5.3±1.1 in controls; P<0.05), indicating a reduction inΔψm. More activated caspase-3-positive VYS cells were present in the diabetic group(50.3±2.8) vs the controls (3.05±1.4; P<0.05; Figure 2B).


Rat visceral yolk sac cells: viability and expression of cell markers during maternal diabetes.

Aires MB, Santos JR, Souza KS, Farias PS, Santos AC, Fioretto ET, Maria DA - Braz. J. Med. Biol. Res. (2015)

A, Flow cytometry plots of visceral yolk sac (VYS) cells fromcontrol and diabetic groups stained with rhodamine 123. The histograms show anincreased number of VYS cells with inactive mitochondria (unviable cells) inthe diabetic group and a similar number of cells with active mitochondria(viable cells) in both groups. B, Representative flowcytometry plot of activated caspase-3 in VYS cells of control and diabeticgroups. The histogram shows a large number of activated caspase-3-expressingVYS cells in the diabetic group. Results are reported as the means±SE of VYScells collected from five yolk sacs per animal of the control (n=5) anddiabetic groups (n=5) analyzed in duplicate. *P<0.05 (Student’st-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4541685&req=5

f02: A, Flow cytometry plots of visceral yolk sac (VYS) cells fromcontrol and diabetic groups stained with rhodamine 123. The histograms show anincreased number of VYS cells with inactive mitochondria (unviable cells) inthe diabetic group and a similar number of cells with active mitochondria(viable cells) in both groups. B, Representative flowcytometry plot of activated caspase-3 in VYS cells of control and diabeticgroups. The histogram shows a large number of activated caspase-3-expressingVYS cells in the diabetic group. Results are reported as the means±SE of VYScells collected from five yolk sacs per animal of the control (n=5) anddiabetic groups (n=5) analyzed in duplicate. *P<0.05 (Student’st-test).
Mentions: Cellular uptake of rhodamine 123 was analyzed and compared between groups (Figure 2A). VYS cells from the diabetic groupshowed a similar number of viable cells with active mitochondria (88.14±1.6) comparedwith the control group (94.1±1.3). However, an increased number of VYS cells withinactive mitochondria (nonviable cells) were present in the diabetic group (12.4±1.5vs 5.3±1.1 in controls; P<0.05), indicating a reduction inΔψm. More activated caspase-3-positive VYS cells were present in the diabetic group(50.3±2.8) vs the controls (3.05±1.4; P<0.05; Figure 2B).

Bottom Line: Fetal weight was reduced in the diabetic group.In the diabetic group, significantly decreased expression of CD34 (P<0.05), CCR2 (P<0.001), and OCT3/4 (P<0.01), and significantly increased expression of CD90 (P<0.05), CD117 (P<0.01), and CD14 (P<0.05) were observed.Severe hyperglycemia caused by maternal diabetes had negative effects on pregnancy, VYS cell viability, and the expression of cell markers.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Morfologia, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.

ABSTRACT
The function of the visceral yolk sac (VYS) is critical for embryo organogenesis until final fetal development in rats, and can be affected by conditions such as diabetes. In view of the importance of diabetes during pregnancy for maternal and neonatal health, the objective of this study was to assess fetal weight, VYS cell markers, and viability in female Wistar rats (200-250 g) with induced diabetes (alloxan, 37 mg/kg) on the 8th gestational day (gd 8). At gd 15, rats from control (n=5) and diabetic (n=5) groups were anesthetized and laparotomized to remove the uterine horns for weighing of fetuses and collecting the VYS. Flow cytometry was used for characterizing VYS cells, and for determining mitochondrial activity, cell proliferation, DNA ploidy, cell cycle phases, and caspase-3 activity. Fetal weight was reduced in the diabetic group. Expression of the cell markers CD34, VEGFR1, CD115, CD117, CD14, CCR2, CD90, CD44, STRO-1, OCT3/4, and Nanog was detected in VYS cells in both groups. In the diabetic group, significantly decreased expression of CD34 (P<0.05), CCR2 (P<0.001), and OCT3/4 (P<0.01), and significantly increased expression of CD90 (P<0.05), CD117 (P<0.01), and CD14 (P<0.05) were observed. VYS cells with inactive mitochondria, activated caspase-3, and low proliferation were present in the rats with diabetes. Severe hyperglycemia caused by maternal diabetes had negative effects on pregnancy, VYS cell viability, and the expression of cell markers.

No MeSH data available.


Related in: MedlinePlus