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Rat visceral yolk sac cells: viability and expression of cell markers during maternal diabetes.

Aires MB, Santos JR, Souza KS, Farias PS, Santos AC, Fioretto ET, Maria DA - Braz. J. Med. Biol. Res. (2015)

Bottom Line: Fetal weight was reduced in the diabetic group.In the diabetic group, significantly decreased expression of CD34 (P<0.05), CCR2 (P<0.001), and OCT3/4 (P<0.01), and significantly increased expression of CD90 (P<0.05), CD117 (P<0.01), and CD14 (P<0.05) were observed.Severe hyperglycemia caused by maternal diabetes had negative effects on pregnancy, VYS cell viability, and the expression of cell markers.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Morfologia, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.

ABSTRACT
The function of the visceral yolk sac (VYS) is critical for embryo organogenesis until final fetal development in rats, and can be affected by conditions such as diabetes. In view of the importance of diabetes during pregnancy for maternal and neonatal health, the objective of this study was to assess fetal weight, VYS cell markers, and viability in female Wistar rats (200-250 g) with induced diabetes (alloxan, 37 mg/kg) on the 8th gestational day (gd 8). At gd 15, rats from control (n=5) and diabetic (n=5) groups were anesthetized and laparotomized to remove the uterine horns for weighing of fetuses and collecting the VYS. Flow cytometry was used for characterizing VYS cells, and for determining mitochondrial activity, cell proliferation, DNA ploidy, cell cycle phases, and caspase-3 activity. Fetal weight was reduced in the diabetic group. Expression of the cell markers CD34, VEGFR1, CD115, CD117, CD14, CCR2, CD90, CD44, STRO-1, OCT3/4, and Nanog was detected in VYS cells in both groups. In the diabetic group, significantly decreased expression of CD34 (P<0.05), CCR2 (P<0.001), and OCT3/4 (P<0.01), and significantly increased expression of CD90 (P<0.05), CD117 (P<0.01), and CD14 (P<0.05) were observed. VYS cells with inactive mitochondria, activated caspase-3, and low proliferation were present in the rats with diabetes. Severe hyperglycemia caused by maternal diabetes had negative effects on pregnancy, VYS cell viability, and the expression of cell markers.

No MeSH data available.


Related in: MedlinePlus

Representative flow cytometry plots of VYS cells from the control anddiabetic groups. These were stained with markers for hematopoiesis (CD34,VEGFR1, CD117), monocytes/macrophages (CD115, CD14, CCR2), stromal tissue(CD90, CD44), and pluripotency (STRO-1, OCT3/4, Nanog). The expression of CD34,CCR2, and OCT3/4 was significantly reduced in the diabetic group. Thehistograms show the increased expression of CD90, CD117 and CD14 in thediabetic group compared with the control. Results are reported as the means±SEof cells collected from five yolk sacs per animal of the control (n=5) anddiabetic groups (n=5) analyzed in duplicate. *P<0.05 (ANOVA and Tukey'smultiple comparison test).
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f01: Representative flow cytometry plots of VYS cells from the control anddiabetic groups. These were stained with markers for hematopoiesis (CD34,VEGFR1, CD117), monocytes/macrophages (CD115, CD14, CCR2), stromal tissue(CD90, CD44), and pluripotency (STRO-1, OCT3/4, Nanog). The expression of CD34,CCR2, and OCT3/4 was significantly reduced in the diabetic group. Thehistograms show the increased expression of CD90, CD117 and CD14 in thediabetic group compared with the control. Results are reported as the means±SEof cells collected from five yolk sacs per animal of the control (n=5) anddiabetic groups (n=5) analyzed in duplicate. *P<0.05 (ANOVA and Tukey'smultiple comparison test).

Mentions: Expression of the VYS cell markers CD34, VEGFR1, CD115, CD117, CD14, CCR2, CD90,CD44, STRO-1, OCT3/4, and Nanog were detected on VYS cells in both the control anddiabetic groups (Figure 1). The expressionlevels were significantly reduced in the diabetic group: CD34 (39.2±0.8vs 27.8±1.0, P<0.05), CCR2 (57.7±0.2 vs34.5±0.5, P<0.001), and OCT3/4 (54.2±0.8 vs 47.9±0.6, P<0.01).Significantly increased expression levels of CD90 (53.4±0.9 vs67.9±1.5, P<0.05), CD117 (40.5±0.3 vs 57.6±0.3, P<0.01) andCD14 (2.5±0.1 vs 18.7±0.7, P<0.05) were measured in the diabeticcompared with the control groups, respectively.


Rat visceral yolk sac cells: viability and expression of cell markers during maternal diabetes.

Aires MB, Santos JR, Souza KS, Farias PS, Santos AC, Fioretto ET, Maria DA - Braz. J. Med. Biol. Res. (2015)

Representative flow cytometry plots of VYS cells from the control anddiabetic groups. These were stained with markers for hematopoiesis (CD34,VEGFR1, CD117), monocytes/macrophages (CD115, CD14, CCR2), stromal tissue(CD90, CD44), and pluripotency (STRO-1, OCT3/4, Nanog). The expression of CD34,CCR2, and OCT3/4 was significantly reduced in the diabetic group. Thehistograms show the increased expression of CD90, CD117 and CD14 in thediabetic group compared with the control. Results are reported as the means±SEof cells collected from five yolk sacs per animal of the control (n=5) anddiabetic groups (n=5) analyzed in duplicate. *P<0.05 (ANOVA and Tukey'smultiple comparison test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4541685&req=5

f01: Representative flow cytometry plots of VYS cells from the control anddiabetic groups. These were stained with markers for hematopoiesis (CD34,VEGFR1, CD117), monocytes/macrophages (CD115, CD14, CCR2), stromal tissue(CD90, CD44), and pluripotency (STRO-1, OCT3/4, Nanog). The expression of CD34,CCR2, and OCT3/4 was significantly reduced in the diabetic group. Thehistograms show the increased expression of CD90, CD117 and CD14 in thediabetic group compared with the control. Results are reported as the means±SEof cells collected from five yolk sacs per animal of the control (n=5) anddiabetic groups (n=5) analyzed in duplicate. *P<0.05 (ANOVA and Tukey'smultiple comparison test).
Mentions: Expression of the VYS cell markers CD34, VEGFR1, CD115, CD117, CD14, CCR2, CD90,CD44, STRO-1, OCT3/4, and Nanog were detected on VYS cells in both the control anddiabetic groups (Figure 1). The expressionlevels were significantly reduced in the diabetic group: CD34 (39.2±0.8vs 27.8±1.0, P<0.05), CCR2 (57.7±0.2 vs34.5±0.5, P<0.001), and OCT3/4 (54.2±0.8 vs 47.9±0.6, P<0.01).Significantly increased expression levels of CD90 (53.4±0.9 vs67.9±1.5, P<0.05), CD117 (40.5±0.3 vs 57.6±0.3, P<0.01) andCD14 (2.5±0.1 vs 18.7±0.7, P<0.05) were measured in the diabeticcompared with the control groups, respectively.

Bottom Line: Fetal weight was reduced in the diabetic group.In the diabetic group, significantly decreased expression of CD34 (P<0.05), CCR2 (P<0.001), and OCT3/4 (P<0.01), and significantly increased expression of CD90 (P<0.05), CD117 (P<0.01), and CD14 (P<0.05) were observed.Severe hyperglycemia caused by maternal diabetes had negative effects on pregnancy, VYS cell viability, and the expression of cell markers.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Morfologia, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.

ABSTRACT
The function of the visceral yolk sac (VYS) is critical for embryo organogenesis until final fetal development in rats, and can be affected by conditions such as diabetes. In view of the importance of diabetes during pregnancy for maternal and neonatal health, the objective of this study was to assess fetal weight, VYS cell markers, and viability in female Wistar rats (200-250 g) with induced diabetes (alloxan, 37 mg/kg) on the 8th gestational day (gd 8). At gd 15, rats from control (n=5) and diabetic (n=5) groups were anesthetized and laparotomized to remove the uterine horns for weighing of fetuses and collecting the VYS. Flow cytometry was used for characterizing VYS cells, and for determining mitochondrial activity, cell proliferation, DNA ploidy, cell cycle phases, and caspase-3 activity. Fetal weight was reduced in the diabetic group. Expression of the cell markers CD34, VEGFR1, CD115, CD117, CD14, CCR2, CD90, CD44, STRO-1, OCT3/4, and Nanog was detected in VYS cells in both groups. In the diabetic group, significantly decreased expression of CD34 (P<0.05), CCR2 (P<0.001), and OCT3/4 (P<0.01), and significantly increased expression of CD90 (P<0.05), CD117 (P<0.01), and CD14 (P<0.05) were observed. VYS cells with inactive mitochondria, activated caspase-3, and low proliferation were present in the rats with diabetes. Severe hyperglycemia caused by maternal diabetes had negative effects on pregnancy, VYS cell viability, and the expression of cell markers.

No MeSH data available.


Related in: MedlinePlus