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TWIK-Related Spinal Cord K⁺ Channel Expression Is Increased in the Spinal Dorsal Horn after Spinal Nerve Ligation.

Hwang HY, Zhang E, Park S, Chung W, Lee S, Kim DW, Ko Y, Lee W - Yonsei Med. J. (2015)

Bottom Line: The SNL group showed significantly higher expression of TRESK in the ipsilateral dorsal horn under pain, but low expression in L5 DRG.Double immunofluorescence staining revealed that immunoreactivity of TRESK was mostly restricted in neuronal cells, and that synapse markers GAD67 and VGlut2 appeared to be associated with TRESK expression.We were unable to find a significant association between TRESK and calcineurin by double immunofluorescence.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesia and Pain Medicine, Chungnam National University Medical School, Daejeon, Korea.

ABSTRACT

Purpose: The TWIK-related spinal cord K⁺ channel (TRESK) has recently been discovered and plays an important role in nociceptor excitability in the pain pathway. Because there have been no reports on the TRESK expression or its function in the dorsal horn of the spinal cord in neuropathic pain, we analyzed TRESK expression in the spinal dorsal horn in a spinal nerve ligation (SNL) model.

Materials and methods: We established a SNL mouse model by using the L5-6 spinal nerves ligation. We used real-time polymerase chain reaction and immunohistochemistry to investigate TRESK expression in the dorsal horn and L5 dorsal rot ganglion (DRG).

Results: The SNL group showed significantly higher expression of TRESK in the ipsilateral dorsal horn under pain, but low expression in L5 DRG. Double immunofluorescence staining revealed that immunoreactivity of TRESK was mostly restricted in neuronal cells, and that synapse markers GAD67 and VGlut2 appeared to be associated with TRESK expression. We were unable to find a significant association between TRESK and calcineurin by double immunofluorescence.

Conclusion: TRESK in spinal cord neurons may contribute to the development of neuropathic pain following injury.

No MeSH data available.


Related in: MedlinePlus

Immunofluorescence staining of TRESK (red) and calcineurin α (green) in the spinal dorsal horn of SNL rats. TRESK expression was not regulated exclusively by calcineurin in the ipsilateral L5-6 region of SNL rats. Scale bars=50 µm in A-D; 20 µm in E-H. TRESK, TWIK-related spinal cord K+; SNL, spinal nerve ligation.
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Figure 9: Immunofluorescence staining of TRESK (red) and calcineurin α (green) in the spinal dorsal horn of SNL rats. TRESK expression was not regulated exclusively by calcineurin in the ipsilateral L5-6 region of SNL rats. Scale bars=50 µm in A-D; 20 µm in E-H. TRESK, TWIK-related spinal cord K+; SNL, spinal nerve ligation.

Mentions: Czirják and Enyedi13 reported that calcineurin may play a major role in TRESK activation. However, we were unable to find a significant association between TRESK and calcineurin by double immunofluorescence (Fig. 7). qPCR performed 0, 7, 14, 21, and 28 days after SNL in the ipsilateral dorsal horn also showed no significant difference, although there was an increased tendency at 21 days (Fig. 8).


TWIK-Related Spinal Cord K⁺ Channel Expression Is Increased in the Spinal Dorsal Horn after Spinal Nerve Ligation.

Hwang HY, Zhang E, Park S, Chung W, Lee S, Kim DW, Ko Y, Lee W - Yonsei Med. J. (2015)

Immunofluorescence staining of TRESK (red) and calcineurin α (green) in the spinal dorsal horn of SNL rats. TRESK expression was not regulated exclusively by calcineurin in the ipsilateral L5-6 region of SNL rats. Scale bars=50 µm in A-D; 20 µm in E-H. TRESK, TWIK-related spinal cord K+; SNL, spinal nerve ligation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4541660&req=5

Figure 9: Immunofluorescence staining of TRESK (red) and calcineurin α (green) in the spinal dorsal horn of SNL rats. TRESK expression was not regulated exclusively by calcineurin in the ipsilateral L5-6 region of SNL rats. Scale bars=50 µm in A-D; 20 µm in E-H. TRESK, TWIK-related spinal cord K+; SNL, spinal nerve ligation.
Mentions: Czirják and Enyedi13 reported that calcineurin may play a major role in TRESK activation. However, we were unable to find a significant association between TRESK and calcineurin by double immunofluorescence (Fig. 7). qPCR performed 0, 7, 14, 21, and 28 days after SNL in the ipsilateral dorsal horn also showed no significant difference, although there was an increased tendency at 21 days (Fig. 8).

Bottom Line: The SNL group showed significantly higher expression of TRESK in the ipsilateral dorsal horn under pain, but low expression in L5 DRG.Double immunofluorescence staining revealed that immunoreactivity of TRESK was mostly restricted in neuronal cells, and that synapse markers GAD67 and VGlut2 appeared to be associated with TRESK expression.We were unable to find a significant association between TRESK and calcineurin by double immunofluorescence.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesia and Pain Medicine, Chungnam National University Medical School, Daejeon, Korea.

ABSTRACT

Purpose: The TWIK-related spinal cord K⁺ channel (TRESK) has recently been discovered and plays an important role in nociceptor excitability in the pain pathway. Because there have been no reports on the TRESK expression or its function in the dorsal horn of the spinal cord in neuropathic pain, we analyzed TRESK expression in the spinal dorsal horn in a spinal nerve ligation (SNL) model.

Materials and methods: We established a SNL mouse model by using the L5-6 spinal nerves ligation. We used real-time polymerase chain reaction and immunohistochemistry to investigate TRESK expression in the dorsal horn and L5 dorsal rot ganglion (DRG).

Results: The SNL group showed significantly higher expression of TRESK in the ipsilateral dorsal horn under pain, but low expression in L5 DRG. Double immunofluorescence staining revealed that immunoreactivity of TRESK was mostly restricted in neuronal cells, and that synapse markers GAD67 and VGlut2 appeared to be associated with TRESK expression. We were unable to find a significant association between TRESK and calcineurin by double immunofluorescence.

Conclusion: TRESK in spinal cord neurons may contribute to the development of neuropathic pain following injury.

No MeSH data available.


Related in: MedlinePlus