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TWIK-Related Spinal Cord K⁺ Channel Expression Is Increased in the Spinal Dorsal Horn after Spinal Nerve Ligation.

Hwang HY, Zhang E, Park S, Chung W, Lee S, Kim DW, Ko Y, Lee W - Yonsei Med. J. (2015)

Bottom Line: The SNL group showed significantly higher expression of TRESK in the ipsilateral dorsal horn under pain, but low expression in L5 DRG.Double immunofluorescence staining revealed that immunoreactivity of TRESK was mostly restricted in neuronal cells, and that synapse markers GAD67 and VGlut2 appeared to be associated with TRESK expression.We were unable to find a significant association between TRESK and calcineurin by double immunofluorescence.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesia and Pain Medicine, Chungnam National University Medical School, Daejeon, Korea.

ABSTRACT

Purpose: The TWIK-related spinal cord K⁺ channel (TRESK) has recently been discovered and plays an important role in nociceptor excitability in the pain pathway. Because there have been no reports on the TRESK expression or its function in the dorsal horn of the spinal cord in neuropathic pain, we analyzed TRESK expression in the spinal dorsal horn in a spinal nerve ligation (SNL) model.

Materials and methods: We established a SNL mouse model by using the L5-6 spinal nerves ligation. We used real-time polymerase chain reaction and immunohistochemistry to investigate TRESK expression in the dorsal horn and L5 dorsal rot ganglion (DRG).

Results: The SNL group showed significantly higher expression of TRESK in the ipsilateral dorsal horn under pain, but low expression in L5 DRG. Double immunofluorescence staining revealed that immunoreactivity of TRESK was mostly restricted in neuronal cells, and that synapse markers GAD67 and VGlut2 appeared to be associated with TRESK expression. We were unable to find a significant association between TRESK and calcineurin by double immunofluorescence.

Conclusion: TRESK in spinal cord neurons may contribute to the development of neuropathic pain following injury.

No MeSH data available.


Related in: MedlinePlus

TRESK transcript expression increased in the dorsal horn of spinal cord of SNL rats. Quantitative real-time PCR of TRESK gene expression in the ipsilateral L5-6 spinal cord at 0, 7, 14, 21, and 28 days after SNL. Data are presented as the fold change from the control (naïve) mean±SEM, which represented normalized averages derived from the threshold cycles in 6 to 8 ipsilateral samples. *p<0.05 vs. control, †p<0.01 vs. control. TRESK, TWIK-related spinal cord K+; SNL, spinal nerve ligation; PCR, polymerase chain reaction.
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Figure 6: TRESK transcript expression increased in the dorsal horn of spinal cord of SNL rats. Quantitative real-time PCR of TRESK gene expression in the ipsilateral L5-6 spinal cord at 0, 7, 14, 21, and 28 days after SNL. Data are presented as the fold change from the control (naïve) mean±SEM, which represented normalized averages derived from the threshold cycles in 6 to 8 ipsilateral samples. *p<0.05 vs. control, †p<0.01 vs. control. TRESK, TWIK-related spinal cord K+; SNL, spinal nerve ligation; PCR, polymerase chain reaction.

Mentions: TRESK gene expression increased in the ipsilateral lumbar spinal cord at 0, 7, 14, 21, and 28 days after SNL in rats. TRESK transcript expression increased in the dorsal horn of SNL rats (p<0.05) (Fig. 5).


TWIK-Related Spinal Cord K⁺ Channel Expression Is Increased in the Spinal Dorsal Horn after Spinal Nerve Ligation.

Hwang HY, Zhang E, Park S, Chung W, Lee S, Kim DW, Ko Y, Lee W - Yonsei Med. J. (2015)

TRESK transcript expression increased in the dorsal horn of spinal cord of SNL rats. Quantitative real-time PCR of TRESK gene expression in the ipsilateral L5-6 spinal cord at 0, 7, 14, 21, and 28 days after SNL. Data are presented as the fold change from the control (naïve) mean±SEM, which represented normalized averages derived from the threshold cycles in 6 to 8 ipsilateral samples. *p<0.05 vs. control, †p<0.01 vs. control. TRESK, TWIK-related spinal cord K+; SNL, spinal nerve ligation; PCR, polymerase chain reaction.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC4541660&req=5

Figure 6: TRESK transcript expression increased in the dorsal horn of spinal cord of SNL rats. Quantitative real-time PCR of TRESK gene expression in the ipsilateral L5-6 spinal cord at 0, 7, 14, 21, and 28 days after SNL. Data are presented as the fold change from the control (naïve) mean±SEM, which represented normalized averages derived from the threshold cycles in 6 to 8 ipsilateral samples. *p<0.05 vs. control, †p<0.01 vs. control. TRESK, TWIK-related spinal cord K+; SNL, spinal nerve ligation; PCR, polymerase chain reaction.
Mentions: TRESK gene expression increased in the ipsilateral lumbar spinal cord at 0, 7, 14, 21, and 28 days after SNL in rats. TRESK transcript expression increased in the dorsal horn of SNL rats (p<0.05) (Fig. 5).

Bottom Line: The SNL group showed significantly higher expression of TRESK in the ipsilateral dorsal horn under pain, but low expression in L5 DRG.Double immunofluorescence staining revealed that immunoreactivity of TRESK was mostly restricted in neuronal cells, and that synapse markers GAD67 and VGlut2 appeared to be associated with TRESK expression.We were unable to find a significant association between TRESK and calcineurin by double immunofluorescence.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesia and Pain Medicine, Chungnam National University Medical School, Daejeon, Korea.

ABSTRACT

Purpose: The TWIK-related spinal cord K⁺ channel (TRESK) has recently been discovered and plays an important role in nociceptor excitability in the pain pathway. Because there have been no reports on the TRESK expression or its function in the dorsal horn of the spinal cord in neuropathic pain, we analyzed TRESK expression in the spinal dorsal horn in a spinal nerve ligation (SNL) model.

Materials and methods: We established a SNL mouse model by using the L5-6 spinal nerves ligation. We used real-time polymerase chain reaction and immunohistochemistry to investigate TRESK expression in the dorsal horn and L5 dorsal rot ganglion (DRG).

Results: The SNL group showed significantly higher expression of TRESK in the ipsilateral dorsal horn under pain, but low expression in L5 DRG. Double immunofluorescence staining revealed that immunoreactivity of TRESK was mostly restricted in neuronal cells, and that synapse markers GAD67 and VGlut2 appeared to be associated with TRESK expression. We were unable to find a significant association between TRESK and calcineurin by double immunofluorescence.

Conclusion: TRESK in spinal cord neurons may contribute to the development of neuropathic pain following injury.

No MeSH data available.


Related in: MedlinePlus