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TWIK-Related Spinal Cord K⁺ Channel Expression Is Increased in the Spinal Dorsal Horn after Spinal Nerve Ligation.

Hwang HY, Zhang E, Park S, Chung W, Lee S, Kim DW, Ko Y, Lee W - Yonsei Med. J. (2015)

Bottom Line: The SNL group showed significantly higher expression of TRESK in the ipsilateral dorsal horn under pain, but low expression in L5 DRG.Double immunofluorescence staining revealed that immunoreactivity of TRESK was mostly restricted in neuronal cells, and that synapse markers GAD67 and VGlut2 appeared to be associated with TRESK expression.We were unable to find a significant association between TRESK and calcineurin by double immunofluorescence.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesia and Pain Medicine, Chungnam National University Medical School, Daejeon, Korea.

ABSTRACT

Purpose: The TWIK-related spinal cord K⁺ channel (TRESK) has recently been discovered and plays an important role in nociceptor excitability in the pain pathway. Because there have been no reports on the TRESK expression or its function in the dorsal horn of the spinal cord in neuropathic pain, we analyzed TRESK expression in the spinal dorsal horn in a spinal nerve ligation (SNL) model.

Materials and methods: We established a SNL mouse model by using the L5-6 spinal nerves ligation. We used real-time polymerase chain reaction and immunohistochemistry to investigate TRESK expression in the dorsal horn and L5 dorsal rot ganglion (DRG).

Results: The SNL group showed significantly higher expression of TRESK in the ipsilateral dorsal horn under pain, but low expression in L5 DRG. Double immunofluorescence staining revealed that immunoreactivity of TRESK was mostly restricted in neuronal cells, and that synapse markers GAD67 and VGlut2 appeared to be associated with TRESK expression. We were unable to find a significant association between TRESK and calcineurin by double immunofluorescence.

Conclusion: TRESK in spinal cord neurons may contribute to the development of neuropathic pain following injury.

No MeSH data available.


Related in: MedlinePlus

TRESK immunoreactivity was similar in the spinal dorsal horn of the sham group. The bilateral region of the sham group showed similar TRESK expression. Scale bars=100 µm in A; 50 µm in B and C. TRESK, TWIK-related spinal cord K+.
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Figure 2: TRESK immunoreactivity was similar in the spinal dorsal horn of the sham group. The bilateral region of the sham group showed similar TRESK expression. Scale bars=100 µm in A; 50 µm in B and C. TRESK, TWIK-related spinal cord K+.

Mentions: TRESK was expressed in the dorsal horn of the spinal cord. The bilateral region of the sham group and the contralateral region of the SNL group showed similar TRESK expression (Fig. 2-1). However, the SNL group showed significantly higher expression of TRESK in the ipsilateral region under pain in contrast to the contralateral region (Fig. 2-2). Increased TRESK expression was most prominent in the SDH (laminae I-II, p<0.001) (Fig. 2-2D). In short, TRESK expression significantly increased in the ipsilateral region where mechanical allodynia due to SNL occurred, and this was most prominent in the SDH.


TWIK-Related Spinal Cord K⁺ Channel Expression Is Increased in the Spinal Dorsal Horn after Spinal Nerve Ligation.

Hwang HY, Zhang E, Park S, Chung W, Lee S, Kim DW, Ko Y, Lee W - Yonsei Med. J. (2015)

TRESK immunoreactivity was similar in the spinal dorsal horn of the sham group. The bilateral region of the sham group showed similar TRESK expression. Scale bars=100 µm in A; 50 µm in B and C. TRESK, TWIK-related spinal cord K+.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4541660&req=5

Figure 2: TRESK immunoreactivity was similar in the spinal dorsal horn of the sham group. The bilateral region of the sham group showed similar TRESK expression. Scale bars=100 µm in A; 50 µm in B and C. TRESK, TWIK-related spinal cord K+.
Mentions: TRESK was expressed in the dorsal horn of the spinal cord. The bilateral region of the sham group and the contralateral region of the SNL group showed similar TRESK expression (Fig. 2-1). However, the SNL group showed significantly higher expression of TRESK in the ipsilateral region under pain in contrast to the contralateral region (Fig. 2-2). Increased TRESK expression was most prominent in the SDH (laminae I-II, p<0.001) (Fig. 2-2D). In short, TRESK expression significantly increased in the ipsilateral region where mechanical allodynia due to SNL occurred, and this was most prominent in the SDH.

Bottom Line: The SNL group showed significantly higher expression of TRESK in the ipsilateral dorsal horn under pain, but low expression in L5 DRG.Double immunofluorescence staining revealed that immunoreactivity of TRESK was mostly restricted in neuronal cells, and that synapse markers GAD67 and VGlut2 appeared to be associated with TRESK expression.We were unable to find a significant association between TRESK and calcineurin by double immunofluorescence.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesia and Pain Medicine, Chungnam National University Medical School, Daejeon, Korea.

ABSTRACT

Purpose: The TWIK-related spinal cord K⁺ channel (TRESK) has recently been discovered and plays an important role in nociceptor excitability in the pain pathway. Because there have been no reports on the TRESK expression or its function in the dorsal horn of the spinal cord in neuropathic pain, we analyzed TRESK expression in the spinal dorsal horn in a spinal nerve ligation (SNL) model.

Materials and methods: We established a SNL mouse model by using the L5-6 spinal nerves ligation. We used real-time polymerase chain reaction and immunohistochemistry to investigate TRESK expression in the dorsal horn and L5 dorsal rot ganglion (DRG).

Results: The SNL group showed significantly higher expression of TRESK in the ipsilateral dorsal horn under pain, but low expression in L5 DRG. Double immunofluorescence staining revealed that immunoreactivity of TRESK was mostly restricted in neuronal cells, and that synapse markers GAD67 and VGlut2 appeared to be associated with TRESK expression. We were unable to find a significant association between TRESK and calcineurin by double immunofluorescence.

Conclusion: TRESK in spinal cord neurons may contribute to the development of neuropathic pain following injury.

No MeSH data available.


Related in: MedlinePlus