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Association of IL13R alpha 1 +1398A/G polymorphism in a North Indian population with asthma: A case-control study.

Sinha S, Singh J, Jindal SK, Birbian N - Allergy Rhinol (Providence) (2015)

Bottom Line: Interleukin 13 (IL13) is directly involved in the secretion of total serum immunoglobulin E (IgE), which plays a major role in the asthma pathogenesis.One of the polymorphic receptor of IL13 is IL13Rα1, which after binding to IL13, initiates signal transduction that results in mucin secretion, airway hyperreactivity, fibrosis, and chitinase up-regulation, which increases asthma risk.Furthermore, the phenotypic characteristics also reveal a significant association with the disease (p < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Panjab University, Chandigarh, India.

ABSTRACT

Background: Interleukin 13 (IL13) is directly involved in the secretion of total serum immunoglobulin E (IgE), which plays a major role in the asthma pathogenesis.

Objective: One of the polymorphic receptor of IL13 is IL13Rα1, which after binding to IL13, initiates signal transduction that results in mucin secretion, airway hyperreactivity, fibrosis, and chitinase up-regulation, which increases asthma risk.

Methods: In the present study, the role of IL13Rα1 +1398A/G gene polymorphisms in asthma was detected with a total of 964 individuals, including 483 healthy controls and 481 asthma patients from a North Indian population using polymerase chain reaction-restriction fragment length polymorphism method.

Results: Statistical analysis revealed that the mutant allele (G) is predominant in asthma patients (42.7%) than the controls (38.2%), which shows an increased risk toward asthma with odds ratio = 1.21, 95% confidence interval (1.00-1.45), χ(2) = 4.10 and p = 0.043. Furthermore, the phenotypic characteristics also reveal a significant association with the disease (p < 0.05).

Conclusions: This is the first study conducted in India and +1398A/G polymorphism in noncoding region of IL13Rα1 confer risk toward asthma in the studied population.

No MeSH data available.


Related in: MedlinePlus

Restriction digestion (MseI) products of IL13Rα1 +1398A//G polymorphism on 3% Agarose gel. Lane 1: 20 bp ladder. Lane 2: heterozygous AG genotype (130, 85, and 45 bp). Lane 3: homozygous mutant GG genotype (130 bp). Lane 4: homozygous wild AA genotype (85 and 45 bp).
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Figure 1: Restriction digestion (MseI) products of IL13Rα1 +1398A//G polymorphism on 3% Agarose gel. Lane 1: 20 bp ladder. Lane 2: heterozygous AG genotype (130, 85, and 45 bp). Lane 3: homozygous mutant GG genotype (130 bp). Lane 4: homozygous wild AA genotype (85 and 45 bp).

Mentions: The amplification of the IL13Rα1 +1398A/G polymorphism was done using polymerase chain reaction-restriction fragment length polymorphism method17 using MseI (New England BioLabs, Hitchin, United Kingdom) (Fig. 1). The primer sequences were: Forward 5′-TCAGTGATGGAGATAATTTA-3′ and Reverse 5′-TGAGCTGCCTGTTTATAAAT-3′.


Association of IL13R alpha 1 +1398A/G polymorphism in a North Indian population with asthma: A case-control study.

Sinha S, Singh J, Jindal SK, Birbian N - Allergy Rhinol (Providence) (2015)

Restriction digestion (MseI) products of IL13Rα1 +1398A//G polymorphism on 3% Agarose gel. Lane 1: 20 bp ladder. Lane 2: heterozygous AG genotype (130, 85, and 45 bp). Lane 3: homozygous mutant GG genotype (130 bp). Lane 4: homozygous wild AA genotype (85 and 45 bp).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4541631&req=5

Figure 1: Restriction digestion (MseI) products of IL13Rα1 +1398A//G polymorphism on 3% Agarose gel. Lane 1: 20 bp ladder. Lane 2: heterozygous AG genotype (130, 85, and 45 bp). Lane 3: homozygous mutant GG genotype (130 bp). Lane 4: homozygous wild AA genotype (85 and 45 bp).
Mentions: The amplification of the IL13Rα1 +1398A/G polymorphism was done using polymerase chain reaction-restriction fragment length polymorphism method17 using MseI (New England BioLabs, Hitchin, United Kingdom) (Fig. 1). The primer sequences were: Forward 5′-TCAGTGATGGAGATAATTTA-3′ and Reverse 5′-TGAGCTGCCTGTTTATAAAT-3′.

Bottom Line: Interleukin 13 (IL13) is directly involved in the secretion of total serum immunoglobulin E (IgE), which plays a major role in the asthma pathogenesis.One of the polymorphic receptor of IL13 is IL13Rα1, which after binding to IL13, initiates signal transduction that results in mucin secretion, airway hyperreactivity, fibrosis, and chitinase up-regulation, which increases asthma risk.Furthermore, the phenotypic characteristics also reveal a significant association with the disease (p < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Panjab University, Chandigarh, India.

ABSTRACT

Background: Interleukin 13 (IL13) is directly involved in the secretion of total serum immunoglobulin E (IgE), which plays a major role in the asthma pathogenesis.

Objective: One of the polymorphic receptor of IL13 is IL13Rα1, which after binding to IL13, initiates signal transduction that results in mucin secretion, airway hyperreactivity, fibrosis, and chitinase up-regulation, which increases asthma risk.

Methods: In the present study, the role of IL13Rα1 +1398A/G gene polymorphisms in asthma was detected with a total of 964 individuals, including 483 healthy controls and 481 asthma patients from a North Indian population using polymerase chain reaction-restriction fragment length polymorphism method.

Results: Statistical analysis revealed that the mutant allele (G) is predominant in asthma patients (42.7%) than the controls (38.2%), which shows an increased risk toward asthma with odds ratio = 1.21, 95% confidence interval (1.00-1.45), χ(2) = 4.10 and p = 0.043. Furthermore, the phenotypic characteristics also reveal a significant association with the disease (p < 0.05).

Conclusions: This is the first study conducted in India and +1398A/G polymorphism in noncoding region of IL13Rα1 confer risk toward asthma in the studied population.

No MeSH data available.


Related in: MedlinePlus