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Identification of the minimal binding region of a Plasmodium falciparum IgM binding PfEMP1 domain.

Semblat JP, Ghumra A, Czajkowsky DM, Wallis R, Mitchell DA, Raza A, Rowe JA - Mol. Biochem. Parasitol. (2015)

Bottom Line: Here we studied an IgM binding domain from a rosette-mediating PfEMP1 variant, DBL4ζ of TM284var1, and found that the minimal IgM binding region mapped to the central region of the DBL domain, comprising all of subdomain 2 and adjoining parts of subdomains 1 and 3.Site-directed mutagenesis of charged amino acids within subdomain 2, predicted by molecular modelling to form the IgM binding site, showed no marked effect on IgM binding properties.Further work is needed to identify the specific interaction site for IgM within the minimal binding region of PfEMP1.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology and Infection Research, Centre for Immunity, Infection and Evolution, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3FL, United Kingdom.

No MeSH data available.


Related in: MedlinePlus

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Identification of the minimal binding region of a Plasmodium falciparum IgM binding PfEMP1 domain.

Semblat JP, Ghumra A, Czajkowsky DM, Wallis R, Mitchell DA, Raza A, Rowe JA - Mol. Biochem. Parasitol. (2015)

© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4539346&req=5

Bottom Line: Here we studied an IgM binding domain from a rosette-mediating PfEMP1 variant, DBL4ζ of TM284var1, and found that the minimal IgM binding region mapped to the central region of the DBL domain, comprising all of subdomain 2 and adjoining parts of subdomains 1 and 3.Site-directed mutagenesis of charged amino acids within subdomain 2, predicted by molecular modelling to form the IgM binding site, showed no marked effect on IgM binding properties.Further work is needed to identify the specific interaction site for IgM within the minimal binding region of PfEMP1.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology and Infection Research, Centre for Immunity, Infection and Evolution, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3FL, United Kingdom.

No MeSH data available.


Related in: MedlinePlus