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Alginate as a protease inhibitor in vitro and in a model gut system; selective inhibition of pepsin but not trypsin.

Chater PI, Wilcox MD, Brownlee IA, Pearson JP - Carbohydr Polym (2015)

Bottom Line: Alginates were shown to reduce pepsin activity by up to 53.9% (±9.5SD) in vitro.Limited inhibition of trypsin was shown.Significant inhibition of proteolysis was shown in the gastric phase of digestion, but not the small intestinal phase.

View Article: PubMed Central - PubMed

Affiliation: Institute for Cell and Molecular Biosciences (ICaMB), Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom. Electronic address: peter.chater@ncl.ac.uk.

No MeSH data available.


Related in: MedlinePlus

Bovine serum albumin (BSA) digestion in the small-intestinal phase of a model gut system with and without SBTI. The graph shows total protein recovered from model gut system after TCA (trichloroacetic acid) precipitation to stop enzyme activity and remove undigested polypeptides. 1 g BSA was digested alone (control digestion) and in the presence of varying concentrations of SBTI. Control digestion is represented as as (■) and digestion with SBTI at 125 mg as (♦), 250 mg (▾) and 500 mg (▴). All samples were tested in triplicate, errors are shown as standard deviation. With 500 mg SBTI, from T[75] until T[180] inhibition of proteolytic activity was between 90.6 and 100% and statistically significant at all time-points (p < 0.05). With 250 mg SBTI, statistically significant inhibition of over 60.1% was achieved at all timepoints after T[70] (p < 0.05). With 125 mg of SBTI, statistically significant inhibition of proteolytic digestion was achieved between T[70] and T[120] ranging from 59.25 to 100% (p < 0.05), however at T[150] and T[180] the reduction in protein digestion relative to control could not be shown to be statistically significant.
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fig0040: Bovine serum albumin (BSA) digestion in the small-intestinal phase of a model gut system with and without SBTI. The graph shows total protein recovered from model gut system after TCA (trichloroacetic acid) precipitation to stop enzyme activity and remove undigested polypeptides. 1 g BSA was digested alone (control digestion) and in the presence of varying concentrations of SBTI. Control digestion is represented as as (■) and digestion with SBTI at 125 mg as (♦), 250 mg (▾) and 500 mg (▴). All samples were tested in triplicate, errors are shown as standard deviation. With 500 mg SBTI, from T[75] until T[180] inhibition of proteolytic activity was between 90.6 and 100% and statistically significant at all time-points (p < 0.05). With 250 mg SBTI, statistically significant inhibition of over 60.1% was achieved at all timepoints after T[70] (p < 0.05). With 125 mg of SBTI, statistically significant inhibition of proteolytic digestion was achieved between T[70] and T[120] ranging from 59.25 to 100% (p < 0.05), however at T[150] and T[180] the reduction in protein digestion relative to control could not be shown to be statistically significant.

Mentions: Fig. 8 shows a control digestion of 1 g of BSA in the model gut system with soybean trypsin inhibitor (SBTI) was used as the positive inhibition control. Three concentrations of SBTI were tested in the model gut in order to validate the detection of inhibition of proteolysis. The addition of SBTI to the digestive mixture reduced proteolytic activity and reduced the amount of digested protein recovered from the assay in the small intestinal phase. From T[70] onwards the addition of 250 and 500 mg yielded statistically significant inhibition at all timepoints until the end of the assay. Inhibition levels of 23%, 75% and 100% were observed with 125, 250 and 500 mg of SBTI.


Alginate as a protease inhibitor in vitro and in a model gut system; selective inhibition of pepsin but not trypsin.

Chater PI, Wilcox MD, Brownlee IA, Pearson JP - Carbohydr Polym (2015)

Bovine serum albumin (BSA) digestion in the small-intestinal phase of a model gut system with and without SBTI. The graph shows total protein recovered from model gut system after TCA (trichloroacetic acid) precipitation to stop enzyme activity and remove undigested polypeptides. 1 g BSA was digested alone (control digestion) and in the presence of varying concentrations of SBTI. Control digestion is represented as as (■) and digestion with SBTI at 125 mg as (♦), 250 mg (▾) and 500 mg (▴). All samples were tested in triplicate, errors are shown as standard deviation. With 500 mg SBTI, from T[75] until T[180] inhibition of proteolytic activity was between 90.6 and 100% and statistically significant at all time-points (p < 0.05). With 250 mg SBTI, statistically significant inhibition of over 60.1% was achieved at all timepoints after T[70] (p < 0.05). With 125 mg of SBTI, statistically significant inhibition of proteolytic digestion was achieved between T[70] and T[120] ranging from 59.25 to 100% (p < 0.05), however at T[150] and T[180] the reduction in protein digestion relative to control could not be shown to be statistically significant.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4539341&req=5

fig0040: Bovine serum albumin (BSA) digestion in the small-intestinal phase of a model gut system with and without SBTI. The graph shows total protein recovered from model gut system after TCA (trichloroacetic acid) precipitation to stop enzyme activity and remove undigested polypeptides. 1 g BSA was digested alone (control digestion) and in the presence of varying concentrations of SBTI. Control digestion is represented as as (■) and digestion with SBTI at 125 mg as (♦), 250 mg (▾) and 500 mg (▴). All samples were tested in triplicate, errors are shown as standard deviation. With 500 mg SBTI, from T[75] until T[180] inhibition of proteolytic activity was between 90.6 and 100% and statistically significant at all time-points (p < 0.05). With 250 mg SBTI, statistically significant inhibition of over 60.1% was achieved at all timepoints after T[70] (p < 0.05). With 125 mg of SBTI, statistically significant inhibition of proteolytic digestion was achieved between T[70] and T[120] ranging from 59.25 to 100% (p < 0.05), however at T[150] and T[180] the reduction in protein digestion relative to control could not be shown to be statistically significant.
Mentions: Fig. 8 shows a control digestion of 1 g of BSA in the model gut system with soybean trypsin inhibitor (SBTI) was used as the positive inhibition control. Three concentrations of SBTI were tested in the model gut in order to validate the detection of inhibition of proteolysis. The addition of SBTI to the digestive mixture reduced proteolytic activity and reduced the amount of digested protein recovered from the assay in the small intestinal phase. From T[70] onwards the addition of 250 and 500 mg yielded statistically significant inhibition at all timepoints until the end of the assay. Inhibition levels of 23%, 75% and 100% were observed with 125, 250 and 500 mg of SBTI.

Bottom Line: Alginates were shown to reduce pepsin activity by up to 53.9% (±9.5SD) in vitro.Limited inhibition of trypsin was shown.Significant inhibition of proteolysis was shown in the gastric phase of digestion, but not the small intestinal phase.

View Article: PubMed Central - PubMed

Affiliation: Institute for Cell and Molecular Biosciences (ICaMB), Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom. Electronic address: peter.chater@ncl.ac.uk.

No MeSH data available.


Related in: MedlinePlus