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Alginate as a protease inhibitor in vitro and in a model gut system; selective inhibition of pepsin but not trypsin.

Chater PI, Wilcox MD, Brownlee IA, Pearson JP - Carbohydr Polym (2015)

Bottom Line: Alginates were shown to reduce pepsin activity by up to 53.9% (±9.5SD) in vitro.Limited inhibition of trypsin was shown.Significant inhibition of proteolysis was shown in the gastric phase of digestion, but not the small intestinal phase.

View Article: PubMed Central - PubMed

Affiliation: Institute for Cell and Molecular Biosciences (ICaMB), Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom. Electronic address: peter.chater@ncl.ac.uk.

No MeSH data available.


Related in: MedlinePlus

Bovine serum albumin digestion in gastric phase of a model gut system with and without FMC13 alginate. The graph shows total protein recovered from model gut system after TCA (trichloroacetic acid) precipitation to stop enzyme activity and remove undigested polypeptides. 0.5 g BSA was digested alone (control digestion) and in the presence of varying concentrations of FMC13. Control digestion is represented as as (■) and digestion with FMC13 at 125 mg as (♦), 250 mg (▾) and 500 mg (▴). All samples were tested in triplicate, errors are shown as standard deviation. At T[60] by the end of the gastric phase, 125, 250 and 500 mg of FMC13 alginate significantly inhibited the gastric digestion of protein by 23.4% (P = 0.021), 52.2% (P = 0.040) and 43.5% (P = 0.013), respectively (t-test).
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fig0025: Bovine serum albumin digestion in gastric phase of a model gut system with and without FMC13 alginate. The graph shows total protein recovered from model gut system after TCA (trichloroacetic acid) precipitation to stop enzyme activity and remove undigested polypeptides. 0.5 g BSA was digested alone (control digestion) and in the presence of varying concentrations of FMC13. Control digestion is represented as as (■) and digestion with FMC13 at 125 mg as (♦), 250 mg (▾) and 500 mg (▴). All samples were tested in triplicate, errors are shown as standard deviation. At T[60] by the end of the gastric phase, 125, 250 and 500 mg of FMC13 alginate significantly inhibited the gastric digestion of protein by 23.4% (P = 0.021), 52.2% (P = 0.040) and 43.5% (P = 0.013), respectively (t-test).

Mentions: Four alginates from across the F[G] range were tested, and all FMC3, FMC13, SF120 and H120L produced significant inhibition of protein digestion in the gastric phase. By the end of the simulated gastric phase, FMC13 was the weakest of the four alginates tested (Fig. 5). By T[60] after an hour of simulated digestion with 125, 250 and 500 mg of FMC13, proteolytic digestion was reduced by 23.4% (P = 0.021), 52.2% (P = 0.04) and 43.5% (P = 0.013), respectively, as compared to a control.


Alginate as a protease inhibitor in vitro and in a model gut system; selective inhibition of pepsin but not trypsin.

Chater PI, Wilcox MD, Brownlee IA, Pearson JP - Carbohydr Polym (2015)

Bovine serum albumin digestion in gastric phase of a model gut system with and without FMC13 alginate. The graph shows total protein recovered from model gut system after TCA (trichloroacetic acid) precipitation to stop enzyme activity and remove undigested polypeptides. 0.5 g BSA was digested alone (control digestion) and in the presence of varying concentrations of FMC13. Control digestion is represented as as (■) and digestion with FMC13 at 125 mg as (♦), 250 mg (▾) and 500 mg (▴). All samples were tested in triplicate, errors are shown as standard deviation. At T[60] by the end of the gastric phase, 125, 250 and 500 mg of FMC13 alginate significantly inhibited the gastric digestion of protein by 23.4% (P = 0.021), 52.2% (P = 0.040) and 43.5% (P = 0.013), respectively (t-test).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4539341&req=5

fig0025: Bovine serum albumin digestion in gastric phase of a model gut system with and without FMC13 alginate. The graph shows total protein recovered from model gut system after TCA (trichloroacetic acid) precipitation to stop enzyme activity and remove undigested polypeptides. 0.5 g BSA was digested alone (control digestion) and in the presence of varying concentrations of FMC13. Control digestion is represented as as (■) and digestion with FMC13 at 125 mg as (♦), 250 mg (▾) and 500 mg (▴). All samples were tested in triplicate, errors are shown as standard deviation. At T[60] by the end of the gastric phase, 125, 250 and 500 mg of FMC13 alginate significantly inhibited the gastric digestion of protein by 23.4% (P = 0.021), 52.2% (P = 0.040) and 43.5% (P = 0.013), respectively (t-test).
Mentions: Four alginates from across the F[G] range were tested, and all FMC3, FMC13, SF120 and H120L produced significant inhibition of protein digestion in the gastric phase. By the end of the simulated gastric phase, FMC13 was the weakest of the four alginates tested (Fig. 5). By T[60] after an hour of simulated digestion with 125, 250 and 500 mg of FMC13, proteolytic digestion was reduced by 23.4% (P = 0.021), 52.2% (P = 0.04) and 43.5% (P = 0.013), respectively, as compared to a control.

Bottom Line: Alginates were shown to reduce pepsin activity by up to 53.9% (±9.5SD) in vitro.Limited inhibition of trypsin was shown.Significant inhibition of proteolysis was shown in the gastric phase of digestion, but not the small intestinal phase.

View Article: PubMed Central - PubMed

Affiliation: Institute for Cell and Molecular Biosciences (ICaMB), Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom. Electronic address: peter.chater@ncl.ac.uk.

No MeSH data available.


Related in: MedlinePlus