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Alginate as a protease inhibitor in vitro and in a model gut system; selective inhibition of pepsin but not trypsin.

Chater PI, Wilcox MD, Brownlee IA, Pearson JP - Carbohydr Polym (2015)

Bottom Line: Alginates were shown to reduce pepsin activity by up to 53.9% (±9.5SD) in vitro.Limited inhibition of trypsin was shown.Significant inhibition of proteolysis was shown in the gastric phase of digestion, but not the small intestinal phase.

View Article: PubMed Central - PubMed

Affiliation: Institute for Cell and Molecular Biosciences (ICaMB), Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom. Electronic address: peter.chater@ncl.ac.uk.

No MeSH data available.


Related in: MedlinePlus

Correlation of alginate G-residue frequency (F[G]) and level of pepsin inhibition with 5 mg/ml alginate. Pepsin activity is shown as a percentage of control pepsin activity. The error bars show the standard deviation of six replicates (n = 6). The line of best fit indicates a negative correlation which is significant with a Spearman r value of −0.7789 and a p value of 0.0001.
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fig0005: Correlation of alginate G-residue frequency (F[G]) and level of pepsin inhibition with 5 mg/ml alginate. Pepsin activity is shown as a percentage of control pepsin activity. The error bars show the standard deviation of six replicates (n = 6). The line of best fit indicates a negative correlation which is significant with a Spearman r value of −0.7789 and a p value of 0.0001.

Mentions: Alginate structure was correlated with percentage pepsin inhibition. A significant negative correlation was shown between pepsin inhibition and alginate F[G] at 5 mg/ml (Fig. 1). This indicates that at 5 mg/ml, an increasing proportion of mannuronic acid residues, and decreasing proportion of guluronic acid residues yielded higher levels of pepsin inhibition.


Alginate as a protease inhibitor in vitro and in a model gut system; selective inhibition of pepsin but not trypsin.

Chater PI, Wilcox MD, Brownlee IA, Pearson JP - Carbohydr Polym (2015)

Correlation of alginate G-residue frequency (F[G]) and level of pepsin inhibition with 5 mg/ml alginate. Pepsin activity is shown as a percentage of control pepsin activity. The error bars show the standard deviation of six replicates (n = 6). The line of best fit indicates a negative correlation which is significant with a Spearman r value of −0.7789 and a p value of 0.0001.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4539341&req=5

fig0005: Correlation of alginate G-residue frequency (F[G]) and level of pepsin inhibition with 5 mg/ml alginate. Pepsin activity is shown as a percentage of control pepsin activity. The error bars show the standard deviation of six replicates (n = 6). The line of best fit indicates a negative correlation which is significant with a Spearman r value of −0.7789 and a p value of 0.0001.
Mentions: Alginate structure was correlated with percentage pepsin inhibition. A significant negative correlation was shown between pepsin inhibition and alginate F[G] at 5 mg/ml (Fig. 1). This indicates that at 5 mg/ml, an increasing proportion of mannuronic acid residues, and decreasing proportion of guluronic acid residues yielded higher levels of pepsin inhibition.

Bottom Line: Alginates were shown to reduce pepsin activity by up to 53.9% (±9.5SD) in vitro.Limited inhibition of trypsin was shown.Significant inhibition of proteolysis was shown in the gastric phase of digestion, but not the small intestinal phase.

View Article: PubMed Central - PubMed

Affiliation: Institute for Cell and Molecular Biosciences (ICaMB), Medical School, Newcastle University, Catherine Cookson Building, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom. Electronic address: peter.chater@ncl.ac.uk.

No MeSH data available.


Related in: MedlinePlus