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Modifications of Human Subcutaneous ADMSC after PPARγ Activation and Cold Exposition.

Vargas D, Rosales W, Lizcano F - Stem Cells Int (2015)

Bottom Line: In the present study, we determined the effect of partial agonism of PPARγ and temperature reduction on phenotype and metabolic activity of ADMSCs from human adipose subcutaneous tissue.We found that adipocytes differentiated with total and partial agonists of PPARγ and exposed to 31°C are able to respond to cold significantly increasing the expression of thermogenic proteins such as UCP1, PGC1α, and CITED1, a marker of beige phenotype.Additionally, we found that adipocyte cells subjected to cold had a reduction in triglycerides and increased adiponectin levels.

View Article: PubMed Central - PubMed

Affiliation: Centro de Investigación Biomédica, CIBUS, Universidad de La Sabana, Km 7, Autopista Norte de Bogotá, 140013 Chía, Colombia.

ABSTRACT
Mesenchymal stem cells are a diverse population of cells with a wide range of potential therapeutic applications. In particular, cells from adipose tissue have the distinction of being easily accessible and contain a lot of stem cells. ADMSCs can be induced to mature adipocyte and activate the energy expenditure upon treatment with total PPARγ agonists. Additionally these cells may respond to cold by activating the thermogenic program. In the present study, we determined the effect of partial agonism of PPARγ and temperature reduction on phenotype and metabolic activity of ADMSCs from human adipose subcutaneous tissue. We found that adipocytes differentiated with total and partial agonists of PPARγ and exposed to 31°C are able to respond to cold significantly increasing the expression of thermogenic proteins such as UCP1, PGC1α, and CITED1, a marker of beige phenotype. Additionally, we found that adipocyte cells subjected to cold had a reduction in triglycerides and increased adiponectin levels. These data confirm the promising role of ADMSCs as a treatment for metabolic disorders since it is possible to induce them to mature adipocytes and modulate their phenotype toward a cell with high-energy expenditure and metabolic beneficial effect.

No MeSH data available.


Related in: MedlinePlus

Effect of IL4 in the levels of adipocytokines. (a) The differentiated adipocytes were obtained and treated as describe before; total protein was isolated for detecting expression of adiponectin and PGC-1α by western blot. IL4 was added to cells during a period of 4 hours and at the same time cells were exposed to 37°C or 31°C. (b) Relative intensity levels were determined by densitometry of bands. Analyses were performed using ANOVA test. Data are expressed as mean ± SD and differences were considered significant with P < 0.05. ∗The differences between the cells treated with IL4.
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fig4: Effect of IL4 in the levels of adipocytokines. (a) The differentiated adipocytes were obtained and treated as describe before; total protein was isolated for detecting expression of adiponectin and PGC-1α by western blot. IL4 was added to cells during a period of 4 hours and at the same time cells were exposed to 37°C or 31°C. (b) Relative intensity levels were determined by densitometry of bands. Analyses were performed using ANOVA test. Data are expressed as mean ± SD and differences were considered significant with P < 0.05. ∗The differences between the cells treated with IL4.

Mentions: Recently a new population of regulatory cells that mediate cold response was observed in mice alternatively activated (type 2/M2) macrophages. When activated by eosinophils via IL4 and IL13 signaling, M2 macrophages are recruited to subcutaneous WAT and secrete catecholamines to activate WAT browning [20, 29, 30]. We treated the differentiated adipose cells from ADMSCs with IL4 during a period of 4 hrs at 37°C and 31°C. IL4 increased the expression of adiponectin and PGC-1α and temperature reduction had synergistic effect with IL4 (Figures 4(a) and 4(b)). This leads us to make the hypothesis that IL4 is an important factor in the process of browning of scWAT from ADMSCs.


Modifications of Human Subcutaneous ADMSC after PPARγ Activation and Cold Exposition.

Vargas D, Rosales W, Lizcano F - Stem Cells Int (2015)

Effect of IL4 in the levels of adipocytokines. (a) The differentiated adipocytes were obtained and treated as describe before; total protein was isolated for detecting expression of adiponectin and PGC-1α by western blot. IL4 was added to cells during a period of 4 hours and at the same time cells were exposed to 37°C or 31°C. (b) Relative intensity levels were determined by densitometry of bands. Analyses were performed using ANOVA test. Data are expressed as mean ± SD and differences were considered significant with P < 0.05. ∗The differences between the cells treated with IL4.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4539182&req=5

fig4: Effect of IL4 in the levels of adipocytokines. (a) The differentiated adipocytes were obtained and treated as describe before; total protein was isolated for detecting expression of adiponectin and PGC-1α by western blot. IL4 was added to cells during a period of 4 hours and at the same time cells were exposed to 37°C or 31°C. (b) Relative intensity levels were determined by densitometry of bands. Analyses were performed using ANOVA test. Data are expressed as mean ± SD and differences were considered significant with P < 0.05. ∗The differences between the cells treated with IL4.
Mentions: Recently a new population of regulatory cells that mediate cold response was observed in mice alternatively activated (type 2/M2) macrophages. When activated by eosinophils via IL4 and IL13 signaling, M2 macrophages are recruited to subcutaneous WAT and secrete catecholamines to activate WAT browning [20, 29, 30]. We treated the differentiated adipose cells from ADMSCs with IL4 during a period of 4 hrs at 37°C and 31°C. IL4 increased the expression of adiponectin and PGC-1α and temperature reduction had synergistic effect with IL4 (Figures 4(a) and 4(b)). This leads us to make the hypothesis that IL4 is an important factor in the process of browning of scWAT from ADMSCs.

Bottom Line: In the present study, we determined the effect of partial agonism of PPARγ and temperature reduction on phenotype and metabolic activity of ADMSCs from human adipose subcutaneous tissue.We found that adipocytes differentiated with total and partial agonists of PPARγ and exposed to 31°C are able to respond to cold significantly increasing the expression of thermogenic proteins such as UCP1, PGC1α, and CITED1, a marker of beige phenotype.Additionally, we found that adipocyte cells subjected to cold had a reduction in triglycerides and increased adiponectin levels.

View Article: PubMed Central - PubMed

Affiliation: Centro de Investigación Biomédica, CIBUS, Universidad de La Sabana, Km 7, Autopista Norte de Bogotá, 140013 Chía, Colombia.

ABSTRACT
Mesenchymal stem cells are a diverse population of cells with a wide range of potential therapeutic applications. In particular, cells from adipose tissue have the distinction of being easily accessible and contain a lot of stem cells. ADMSCs can be induced to mature adipocyte and activate the energy expenditure upon treatment with total PPARγ agonists. Additionally these cells may respond to cold by activating the thermogenic program. In the present study, we determined the effect of partial agonism of PPARγ and temperature reduction on phenotype and metabolic activity of ADMSCs from human adipose subcutaneous tissue. We found that adipocytes differentiated with total and partial agonists of PPARγ and exposed to 31°C are able to respond to cold significantly increasing the expression of thermogenic proteins such as UCP1, PGC1α, and CITED1, a marker of beige phenotype. Additionally, we found that adipocyte cells subjected to cold had a reduction in triglycerides and increased adiponectin levels. These data confirm the promising role of ADMSCs as a treatment for metabolic disorders since it is possible to induce them to mature adipocytes and modulate their phenotype toward a cell with high-energy expenditure and metabolic beneficial effect.

No MeSH data available.


Related in: MedlinePlus