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Modifications of Human Subcutaneous ADMSC after PPARγ Activation and Cold Exposition.

Vargas D, Rosales W, Lizcano F - Stem Cells Int (2015)

Bottom Line: In the present study, we determined the effect of partial agonism of PPARγ and temperature reduction on phenotype and metabolic activity of ADMSCs from human adipose subcutaneous tissue.We found that adipocytes differentiated with total and partial agonists of PPARγ and exposed to 31°C are able to respond to cold significantly increasing the expression of thermogenic proteins such as UCP1, PGC1α, and CITED1, a marker of beige phenotype.Additionally, we found that adipocyte cells subjected to cold had a reduction in triglycerides and increased adiponectin levels.

View Article: PubMed Central - PubMed

Affiliation: Centro de Investigación Biomédica, CIBUS, Universidad de La Sabana, Km 7, Autopista Norte de Bogotá, 140013 Chía, Colombia.

ABSTRACT
Mesenchymal stem cells are a diverse population of cells with a wide range of potential therapeutic applications. In particular, cells from adipose tissue have the distinction of being easily accessible and contain a lot of stem cells. ADMSCs can be induced to mature adipocyte and activate the energy expenditure upon treatment with total PPARγ agonists. Additionally these cells may respond to cold by activating the thermogenic program. In the present study, we determined the effect of partial agonism of PPARγ and temperature reduction on phenotype and metabolic activity of ADMSCs from human adipose subcutaneous tissue. We found that adipocytes differentiated with total and partial agonists of PPARγ and exposed to 31°C are able to respond to cold significantly increasing the expression of thermogenic proteins such as UCP1, PGC1α, and CITED1, a marker of beige phenotype. Additionally, we found that adipocyte cells subjected to cold had a reduction in triglycerides and increased adiponectin levels. These data confirm the promising role of ADMSCs as a treatment for metabolic disorders since it is possible to induce them to mature adipocytes and modulate their phenotype toward a cell with high-energy expenditure and metabolic beneficial effect.

No MeSH data available.


Related in: MedlinePlus

Effect of cold in the levels of adipocytokines. (a) The differentiated adipocytes were obtained and treated as described in Figure 1; total protein was isolated for detecting expression of adiponectin and FABP4 by western blot. (b) Relative intensity levels were determined by densitometry of bands. Analyses were performed using ANOVA test. Data are expressed as mean ± SD and differences were considered significant with P < 0.05. ∗Differences between total and partial agonist in relation to control. # indicates meaningful differences observed after 4 hours of treatment with agonists after exposure to cold in comparison with adipocytes treated in equal conditions at 37°C. Data were normalized with GAPDH.
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fig3: Effect of cold in the levels of adipocytokines. (a) The differentiated adipocytes were obtained and treated as described in Figure 1; total protein was isolated for detecting expression of adiponectin and FABP4 by western blot. (b) Relative intensity levels were determined by densitometry of bands. Analyses were performed using ANOVA test. Data are expressed as mean ± SD and differences were considered significant with P < 0.05. ∗Differences between total and partial agonist in relation to control. # indicates meaningful differences observed after 4 hours of treatment with agonists after exposure to cold in comparison with adipocytes treated in equal conditions at 37°C. Data were normalized with GAPDH.

Mentions: In comparison to effect of temperature over energy expenditure, the cold consequence on adipocytokines expression has been poorly studied. In the present study, we observed an increase in adiponectin expression in cells that were differentiated by both PPARγ agonists (Figure 3(a)). This observation together with the decrease of the amount of triglycerides as well as an increase in the thermogenic markers of adipose cells directs us to believe that WAT cells from ADMSCs have browning process towards beige adipocytes. It is important to highlight the first occasion in which a reduction in temperature has a beneficial change in adiponectin production by adipose cells derived from ADMSCs, both at baseline and after therapy PPARγ agonists (Figures 3(a) and 3(b)).


Modifications of Human Subcutaneous ADMSC after PPARγ Activation and Cold Exposition.

Vargas D, Rosales W, Lizcano F - Stem Cells Int (2015)

Effect of cold in the levels of adipocytokines. (a) The differentiated adipocytes were obtained and treated as described in Figure 1; total protein was isolated for detecting expression of adiponectin and FABP4 by western blot. (b) Relative intensity levels were determined by densitometry of bands. Analyses were performed using ANOVA test. Data are expressed as mean ± SD and differences were considered significant with P < 0.05. ∗Differences between total and partial agonist in relation to control. # indicates meaningful differences observed after 4 hours of treatment with agonists after exposure to cold in comparison with adipocytes treated in equal conditions at 37°C. Data were normalized with GAPDH.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4539182&req=5

fig3: Effect of cold in the levels of adipocytokines. (a) The differentiated adipocytes were obtained and treated as described in Figure 1; total protein was isolated for detecting expression of adiponectin and FABP4 by western blot. (b) Relative intensity levels were determined by densitometry of bands. Analyses were performed using ANOVA test. Data are expressed as mean ± SD and differences were considered significant with P < 0.05. ∗Differences between total and partial agonist in relation to control. # indicates meaningful differences observed after 4 hours of treatment with agonists after exposure to cold in comparison with adipocytes treated in equal conditions at 37°C. Data were normalized with GAPDH.
Mentions: In comparison to effect of temperature over energy expenditure, the cold consequence on adipocytokines expression has been poorly studied. In the present study, we observed an increase in adiponectin expression in cells that were differentiated by both PPARγ agonists (Figure 3(a)). This observation together with the decrease of the amount of triglycerides as well as an increase in the thermogenic markers of adipose cells directs us to believe that WAT cells from ADMSCs have browning process towards beige adipocytes. It is important to highlight the first occasion in which a reduction in temperature has a beneficial change in adiponectin production by adipose cells derived from ADMSCs, both at baseline and after therapy PPARγ agonists (Figures 3(a) and 3(b)).

Bottom Line: In the present study, we determined the effect of partial agonism of PPARγ and temperature reduction on phenotype and metabolic activity of ADMSCs from human adipose subcutaneous tissue.We found that adipocytes differentiated with total and partial agonists of PPARγ and exposed to 31°C are able to respond to cold significantly increasing the expression of thermogenic proteins such as UCP1, PGC1α, and CITED1, a marker of beige phenotype.Additionally, we found that adipocyte cells subjected to cold had a reduction in triglycerides and increased adiponectin levels.

View Article: PubMed Central - PubMed

Affiliation: Centro de Investigación Biomédica, CIBUS, Universidad de La Sabana, Km 7, Autopista Norte de Bogotá, 140013 Chía, Colombia.

ABSTRACT
Mesenchymal stem cells are a diverse population of cells with a wide range of potential therapeutic applications. In particular, cells from adipose tissue have the distinction of being easily accessible and contain a lot of stem cells. ADMSCs can be induced to mature adipocyte and activate the energy expenditure upon treatment with total PPARγ agonists. Additionally these cells may respond to cold by activating the thermogenic program. In the present study, we determined the effect of partial agonism of PPARγ and temperature reduction on phenotype and metabolic activity of ADMSCs from human adipose subcutaneous tissue. We found that adipocytes differentiated with total and partial agonists of PPARγ and exposed to 31°C are able to respond to cold significantly increasing the expression of thermogenic proteins such as UCP1, PGC1α, and CITED1, a marker of beige phenotype. Additionally, we found that adipocyte cells subjected to cold had a reduction in triglycerides and increased adiponectin levels. These data confirm the promising role of ADMSCs as a treatment for metabolic disorders since it is possible to induce them to mature adipocytes and modulate their phenotype toward a cell with high-energy expenditure and metabolic beneficial effect.

No MeSH data available.


Related in: MedlinePlus