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Mesenchymal Stem Cells for Cardiac Regenerative Therapy: Optimization of Cell Differentiation Strategy.

Shen H, Wang Y, Zhang Z, Yang J, Hu S, Shen Z - Stem Cells Int (2015)

Bottom Line: Conventional treatments cannot regenerate the functional cell efficiently.Recent researches suggest that mesenchymal stem cells (MSCs) are able to differentiate into multiple lineages, including cardiomyocyte-like cells in vitro and in vivo, and they have been used for the treatment of MI to repair the injured myocardium and improve cardiac function.In this review, we will focus on the recent progress on MSCs derived cardiomyocytes for cardiac regeneration after MI.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Surgery and Institute of Cardiovascular Science, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China.

ABSTRACT
With the high mortality rate, coronary heart disease (CHD) has currently become a major life-threatening disease. The main pathological change of myocardial infarction (MI) is the induction of myocardial necrosis in infarction area which finally causes heart failure. Conventional treatments cannot regenerate the functional cell efficiently. Recent researches suggest that mesenchymal stem cells (MSCs) are able to differentiate into multiple lineages, including cardiomyocyte-like cells in vitro and in vivo, and they have been used for the treatment of MI to repair the injured myocardium and improve cardiac function. In this review, we will focus on the recent progress on MSCs derived cardiomyocytes for cardiac regeneration after MI.

No MeSH data available.


Related in: MedlinePlus

The diagram for the induction and identification of cardiomyocyte-like cells. MSCs cultured in medium supplemented with 5-Aza, DMSO, and BMP-2 will be induced to cardiomyocyte-like cells 24 h later. MSCs incubated in CLM/myocardial cell broth will differentiate to cardiomyocyte-like cells after 2 w. MSCs cocultured with cardiomyocyte will differentiated to cardiomyocyte-like cells 7 d later. The identification methods consist of morphology detection and molecular marker analysis.
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Related In: Results  -  Collection


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fig1: The diagram for the induction and identification of cardiomyocyte-like cells. MSCs cultured in medium supplemented with 5-Aza, DMSO, and BMP-2 will be induced to cardiomyocyte-like cells 24 h later. MSCs incubated in CLM/myocardial cell broth will differentiate to cardiomyocyte-like cells after 2 w. MSCs cocultured with cardiomyocyte will differentiated to cardiomyocyte-like cells 7 d later. The identification methods consist of morphology detection and molecular marker analysis.

Mentions: Currently, the major methods to induce myocardial cell from BMSCs include biochemistry induction, myocardial microenvironment induction, and genetic modification (Figure 1).


Mesenchymal Stem Cells for Cardiac Regenerative Therapy: Optimization of Cell Differentiation Strategy.

Shen H, Wang Y, Zhang Z, Yang J, Hu S, Shen Z - Stem Cells Int (2015)

The diagram for the induction and identification of cardiomyocyte-like cells. MSCs cultured in medium supplemented with 5-Aza, DMSO, and BMP-2 will be induced to cardiomyocyte-like cells 24 h later. MSCs incubated in CLM/myocardial cell broth will differentiate to cardiomyocyte-like cells after 2 w. MSCs cocultured with cardiomyocyte will differentiated to cardiomyocyte-like cells 7 d later. The identification methods consist of morphology detection and molecular marker analysis.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4539177&req=5

fig1: The diagram for the induction and identification of cardiomyocyte-like cells. MSCs cultured in medium supplemented with 5-Aza, DMSO, and BMP-2 will be induced to cardiomyocyte-like cells 24 h later. MSCs incubated in CLM/myocardial cell broth will differentiate to cardiomyocyte-like cells after 2 w. MSCs cocultured with cardiomyocyte will differentiated to cardiomyocyte-like cells 7 d later. The identification methods consist of morphology detection and molecular marker analysis.
Mentions: Currently, the major methods to induce myocardial cell from BMSCs include biochemistry induction, myocardial microenvironment induction, and genetic modification (Figure 1).

Bottom Line: Conventional treatments cannot regenerate the functional cell efficiently.Recent researches suggest that mesenchymal stem cells (MSCs) are able to differentiate into multiple lineages, including cardiomyocyte-like cells in vitro and in vivo, and they have been used for the treatment of MI to repair the injured myocardium and improve cardiac function.In this review, we will focus on the recent progress on MSCs derived cardiomyocytes for cardiac regeneration after MI.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Surgery and Institute of Cardiovascular Science, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China.

ABSTRACT
With the high mortality rate, coronary heart disease (CHD) has currently become a major life-threatening disease. The main pathological change of myocardial infarction (MI) is the induction of myocardial necrosis in infarction area which finally causes heart failure. Conventional treatments cannot regenerate the functional cell efficiently. Recent researches suggest that mesenchymal stem cells (MSCs) are able to differentiate into multiple lineages, including cardiomyocyte-like cells in vitro and in vivo, and they have been used for the treatment of MI to repair the injured myocardium and improve cardiac function. In this review, we will focus on the recent progress on MSCs derived cardiomyocytes for cardiac regeneration after MI.

No MeSH data available.


Related in: MedlinePlus