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Elevated Serum Levels of Soluble TNF Receptors and Adhesion Molecules Are Associated with Diabetic Retinopathy in Patients with Type-1 Diabetes.

Sharma S, Purohit S, Sharma A, Hopkins D, Steed L, Bode B, Anderson SW, Caldwell R, She JX - Mediators Inflamm. (2015)

Bottom Line: The levels of sTNFR-I, sTNFR-II, CRP, SAA, sgp130, sIL6R, sVCAM1, and sICAM1 were significantly higher in the T1D patients with DR as compared to T1D patients with no complications.The circulating markers of inflammation, endothelial injury, and TNF signaling are significantly associated with DR in patients with T1D.TNFR-I and TNFR-II receptors are highly correlated, but DR associated more strongly with TNFR-I in these patients.

View Article: PubMed Central - PubMed

Affiliation: Center for Biotechnology and Genomic Medicine, Georgia Regents University, 1120 15th Street, Augusta, GA 30912, USA.

ABSTRACT

Aims: To examine the association of the serum levels of TNF receptors, adhesion molecules, and inflammatory mediators with diabetic retinopathy (DR) in T1D patients.

Methods: Using the multiplex immunoassay, we measured serum levels of eight proteins in 678 T1D subjects aged 20-75 years. Comparisons were made between 482 T1D patients with no complications and 196 T1D patients with DR.

Results: The levels of sTNFR-I, sTNFR-II, CRP, SAA, sgp130, sIL6R, sVCAM1, and sICAM1 were significantly higher in the T1D patients with DR as compared to T1D patients with no complications. Multivariate logistic regression analysis revealed significant association for five proteins after adjustment for age, sex, and disease duration (sTNFR-I: OR = 1.57, sgp130: OR = 1.43, sVCAM1: OR = 1.27, sICAM1: OR = 1.42, and CRP: OR = 1.15). Conditional logistic regression on matched paired data revealed that subjects in the top quartile for sTNFR-I (OR = 2.13), sTNFR-II (OR = 1.66), sgp130 (OR = 1.82), sIL6R (OR = 1.75), sVCAM1 (OR = 1.98), sICAM1 (OR = 2.23), CRP (OR = 2.40) and SAA (OR = 2.03), had the highest odds of having DR.

Conclusions: The circulating markers of inflammation, endothelial injury, and TNF signaling are significantly associated with DR in patients with T1D. TNFR-I and TNFR-II receptors are highly correlated, but DR associated more strongly with TNFR-I in these patients.

No MeSH data available.


Related in: MedlinePlus

Three clusters of functionally related proteins with strong positive correlations. The pairwise correlations between all eight proteins were examined in T1D patients with and without DR separately. Correlation between individual protein levels was computed using Pearson correlation coefficient. Clustering and visualization of correlation matrix was performed using hierarchical clustering method and heatmap. Three clusters of functionally related proteins were found with strong positive correlations.
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fig2: Three clusters of functionally related proteins with strong positive correlations. The pairwise correlations between all eight proteins were examined in T1D patients with and without DR separately. Correlation between individual protein levels was computed using Pearson correlation coefficient. Clustering and visualization of correlation matrix was performed using hierarchical clustering method and heatmap. Three clusters of functionally related proteins were found with strong positive correlations.

Mentions: The levels of all eight molecules were significantly higher in the T1D patients with DR as compared to T1D patients with no complications: sTNFR-I (1.30-fold), sTNFR-II (1.27-fold), CRP (1.53-fold), SAA (1.33-fold), sgp130 (1.14-fold), sIL6R (1.08-fold), sVCAM1 (1.11-fold), and sICAM1 (1.19-fold) as shown in Figure 1. Next, we examined the pairwise correlations between all eight proteins and hierarchical clustering of the correlation matrix was performed in T1D patients with and without DR separately (Figure 2). We found three clusters of functionally related proteins with strong positive correlations. The proteins in cluster-1 include sgp130, sVCAM1, sICAM1, and sIL6R and the proteins in cluster-2 are CRP and SAA. The third cluster of proteins with strong positive correlation includes sTNFR-I and sTNFR-II. The correlations were almost similar in both no complication and DR groups (Figure 2) except sTNFR-II. The correlation of sTNFR-II was increased with other proteins in DR group as compared to the T1D group without any complications.


Elevated Serum Levels of Soluble TNF Receptors and Adhesion Molecules Are Associated with Diabetic Retinopathy in Patients with Type-1 Diabetes.

Sharma S, Purohit S, Sharma A, Hopkins D, Steed L, Bode B, Anderson SW, Caldwell R, She JX - Mediators Inflamm. (2015)

Three clusters of functionally related proteins with strong positive correlations. The pairwise correlations between all eight proteins were examined in T1D patients with and without DR separately. Correlation between individual protein levels was computed using Pearson correlation coefficient. Clustering and visualization of correlation matrix was performed using hierarchical clustering method and heatmap. Three clusters of functionally related proteins were found with strong positive correlations.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4539119&req=5

fig2: Three clusters of functionally related proteins with strong positive correlations. The pairwise correlations between all eight proteins were examined in T1D patients with and without DR separately. Correlation between individual protein levels was computed using Pearson correlation coefficient. Clustering and visualization of correlation matrix was performed using hierarchical clustering method and heatmap. Three clusters of functionally related proteins were found with strong positive correlations.
Mentions: The levels of all eight molecules were significantly higher in the T1D patients with DR as compared to T1D patients with no complications: sTNFR-I (1.30-fold), sTNFR-II (1.27-fold), CRP (1.53-fold), SAA (1.33-fold), sgp130 (1.14-fold), sIL6R (1.08-fold), sVCAM1 (1.11-fold), and sICAM1 (1.19-fold) as shown in Figure 1. Next, we examined the pairwise correlations between all eight proteins and hierarchical clustering of the correlation matrix was performed in T1D patients with and without DR separately (Figure 2). We found three clusters of functionally related proteins with strong positive correlations. The proteins in cluster-1 include sgp130, sVCAM1, sICAM1, and sIL6R and the proteins in cluster-2 are CRP and SAA. The third cluster of proteins with strong positive correlation includes sTNFR-I and sTNFR-II. The correlations were almost similar in both no complication and DR groups (Figure 2) except sTNFR-II. The correlation of sTNFR-II was increased with other proteins in DR group as compared to the T1D group without any complications.

Bottom Line: The levels of sTNFR-I, sTNFR-II, CRP, SAA, sgp130, sIL6R, sVCAM1, and sICAM1 were significantly higher in the T1D patients with DR as compared to T1D patients with no complications.The circulating markers of inflammation, endothelial injury, and TNF signaling are significantly associated with DR in patients with T1D.TNFR-I and TNFR-II receptors are highly correlated, but DR associated more strongly with TNFR-I in these patients.

View Article: PubMed Central - PubMed

Affiliation: Center for Biotechnology and Genomic Medicine, Georgia Regents University, 1120 15th Street, Augusta, GA 30912, USA.

ABSTRACT

Aims: To examine the association of the serum levels of TNF receptors, adhesion molecules, and inflammatory mediators with diabetic retinopathy (DR) in T1D patients.

Methods: Using the multiplex immunoassay, we measured serum levels of eight proteins in 678 T1D subjects aged 20-75 years. Comparisons were made between 482 T1D patients with no complications and 196 T1D patients with DR.

Results: The levels of sTNFR-I, sTNFR-II, CRP, SAA, sgp130, sIL6R, sVCAM1, and sICAM1 were significantly higher in the T1D patients with DR as compared to T1D patients with no complications. Multivariate logistic regression analysis revealed significant association for five proteins after adjustment for age, sex, and disease duration (sTNFR-I: OR = 1.57, sgp130: OR = 1.43, sVCAM1: OR = 1.27, sICAM1: OR = 1.42, and CRP: OR = 1.15). Conditional logistic regression on matched paired data revealed that subjects in the top quartile for sTNFR-I (OR = 2.13), sTNFR-II (OR = 1.66), sgp130 (OR = 1.82), sIL6R (OR = 1.75), sVCAM1 (OR = 1.98), sICAM1 (OR = 2.23), CRP (OR = 2.40) and SAA (OR = 2.03), had the highest odds of having DR.

Conclusions: The circulating markers of inflammation, endothelial injury, and TNF signaling are significantly associated with DR in patients with T1D. TNFR-I and TNFR-II receptors are highly correlated, but DR associated more strongly with TNFR-I in these patients.

No MeSH data available.


Related in: MedlinePlus