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Evaluation of Anti-TBGL Antibody in the Diagnosis of Tuberculosis Patients in China.

Zhao J, Zhu Z, Zhang X, Suzuki Y, Chagan-Yasutan H, Chen H, Wan Y, Xu J, Ashino Y, Hattori T - J Immunol Res (2015)

Bottom Line: However, anti-TBGL IgA measurements were significantly less sensitive in detecting ETB than PTB (15.4% versus 46.7% sensitivity) but showed up to 89.7% specificity.Unlike anti-lipoarabinomannan (LAM) IgG that was found to elevate in sputum smearpositive subjects, anti-TBGL IgG and IgA elevated in those with cavitation and bronchiectasis, respectively.Anti-TBGL IgG in cavitary TB yielded 78.2% sensitivity compared to 57.1% in those otherwise.

View Article: PubMed Central - PubMed

Affiliation: Division of Emerging Infectious Diseases, Department of Internal Medicine, Graduate School of Medicine, Tohoku University, Sendai, Miyagi 980-8574, Japan.

ABSTRACT
Tuberculous glycolipid (TBGL) is a component of the Mycobacterium tuberculosis cell wall, and anti-TBGL antibodies are used for serodiagnosis of tuberculosis. Anti-TBGL IgG and IgA levels were measured in 45 pulmonary TB patients (PTB), 26 extra-pulmonary TB patients (ETB), 16 AIDS-TB patients, and 58 healthy controls (HC) including 39 health care workers (HW) and 19 newly enrolled students (ST). Anti-TBGL IgG measurements yielded 68.9% and 46.2% sensitivity in PTB and ETB, respectively, and 81.0% specificity. However, anti-TBGL IgA measurements were significantly less sensitive in detecting ETB than PTB (15.4% versus 46.7% sensitivity) but showed up to 89.7% specificity. Samples from AIDS-TB patients exhibited low reaction of anti-TBGL IgG and IgA with 6.3% and 12.5% sensitivity, respectively. Unlike anti-lipoarabinomannan (LAM) IgG that was found to elevate in sputum smearpositive subjects, anti-TBGL IgG and IgA elevated in those with cavitation and bronchiectasis, respectively. Anti-TBGL IgG in cavitary TB yielded 78.2% sensitivity compared to 57.1% in those otherwise. Meanwhile, higher anti-TBGL IgA titers were observed in HW than in ST, and increasing anti-TBGL IgG titers were observed in HW on follow-up. Therefore, higher anti-TBGL antibody titers are present in patients presenting cavities and bronchiectasis and subjects under TB exposure risk.

No MeSH data available.


Related in: MedlinePlus

Detection of anti-TBGL antibodies and anti-LAM IgG among HC including HW and ST and follow-up study of HW. HW refers to health care workers; ST refers to new enrolled students (a and b); 2009, 2010, and 2011 refer to different years of sample collection (c and d).
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Related In: Results  -  Collection


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fig4: Detection of anti-TBGL antibodies and anti-LAM IgG among HC including HW and ST and follow-up study of HW. HW refers to health care workers; ST refers to new enrolled students (a and b); 2009, 2010, and 2011 refer to different years of sample collection (c and d).

Mentions: We examined HC and HW anti-TBGL antibody titers. There are two subgroups of HC in this study, HW who worked in environments with higher risk to TB exposure and ST who had merely enrolled less than 1 year in the lab. The age of HW (30.51 ± 0.99, n = 39) was significantly higher than that of ST (24.16 ± 1.38, n = 19) (Table 1). Age did not correlate with either anti-TBGL antibody or anti-LAM IgG titers within HC or ST (Spearman, p > 0.05). The difference between ST and HW for anti-TBGL IgG and anti-LAM IgG titers was not statistically significant (nonparametric t-test, p > 0.05 for both), while such difference for anti-TBGL IgA titers was significant (p < 0.01, Figure 4(b)). We also performed a follow-up study of anti-TBGL antibodies in 16 HW. In the three-year follow-up, we observed a trend of HW subjects with increasing positive for anti-TBGL IgG but not for anti-TBGL IgA (p < 0.05, Figures 4(c) and 4(d)).


Evaluation of Anti-TBGL Antibody in the Diagnosis of Tuberculosis Patients in China.

Zhao J, Zhu Z, Zhang X, Suzuki Y, Chagan-Yasutan H, Chen H, Wan Y, Xu J, Ashino Y, Hattori T - J Immunol Res (2015)

Detection of anti-TBGL antibodies and anti-LAM IgG among HC including HW and ST and follow-up study of HW. HW refers to health care workers; ST refers to new enrolled students (a and b); 2009, 2010, and 2011 refer to different years of sample collection (c and d).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4539117&req=5

fig4: Detection of anti-TBGL antibodies and anti-LAM IgG among HC including HW and ST and follow-up study of HW. HW refers to health care workers; ST refers to new enrolled students (a and b); 2009, 2010, and 2011 refer to different years of sample collection (c and d).
Mentions: We examined HC and HW anti-TBGL antibody titers. There are two subgroups of HC in this study, HW who worked in environments with higher risk to TB exposure and ST who had merely enrolled less than 1 year in the lab. The age of HW (30.51 ± 0.99, n = 39) was significantly higher than that of ST (24.16 ± 1.38, n = 19) (Table 1). Age did not correlate with either anti-TBGL antibody or anti-LAM IgG titers within HC or ST (Spearman, p > 0.05). The difference between ST and HW for anti-TBGL IgG and anti-LAM IgG titers was not statistically significant (nonparametric t-test, p > 0.05 for both), while such difference for anti-TBGL IgA titers was significant (p < 0.01, Figure 4(b)). We also performed a follow-up study of anti-TBGL antibodies in 16 HW. In the three-year follow-up, we observed a trend of HW subjects with increasing positive for anti-TBGL IgG but not for anti-TBGL IgA (p < 0.05, Figures 4(c) and 4(d)).

Bottom Line: However, anti-TBGL IgA measurements were significantly less sensitive in detecting ETB than PTB (15.4% versus 46.7% sensitivity) but showed up to 89.7% specificity.Unlike anti-lipoarabinomannan (LAM) IgG that was found to elevate in sputum smearpositive subjects, anti-TBGL IgG and IgA elevated in those with cavitation and bronchiectasis, respectively.Anti-TBGL IgG in cavitary TB yielded 78.2% sensitivity compared to 57.1% in those otherwise.

View Article: PubMed Central - PubMed

Affiliation: Division of Emerging Infectious Diseases, Department of Internal Medicine, Graduate School of Medicine, Tohoku University, Sendai, Miyagi 980-8574, Japan.

ABSTRACT
Tuberculous glycolipid (TBGL) is a component of the Mycobacterium tuberculosis cell wall, and anti-TBGL antibodies are used for serodiagnosis of tuberculosis. Anti-TBGL IgG and IgA levels were measured in 45 pulmonary TB patients (PTB), 26 extra-pulmonary TB patients (ETB), 16 AIDS-TB patients, and 58 healthy controls (HC) including 39 health care workers (HW) and 19 newly enrolled students (ST). Anti-TBGL IgG measurements yielded 68.9% and 46.2% sensitivity in PTB and ETB, respectively, and 81.0% specificity. However, anti-TBGL IgA measurements were significantly less sensitive in detecting ETB than PTB (15.4% versus 46.7% sensitivity) but showed up to 89.7% specificity. Samples from AIDS-TB patients exhibited low reaction of anti-TBGL IgG and IgA with 6.3% and 12.5% sensitivity, respectively. Unlike anti-lipoarabinomannan (LAM) IgG that was found to elevate in sputum smearpositive subjects, anti-TBGL IgG and IgA elevated in those with cavitation and bronchiectasis, respectively. Anti-TBGL IgG in cavitary TB yielded 78.2% sensitivity compared to 57.1% in those otherwise. Meanwhile, higher anti-TBGL IgA titers were observed in HW than in ST, and increasing anti-TBGL IgG titers were observed in HW on follow-up. Therefore, higher anti-TBGL antibody titers are present in patients presenting cavities and bronchiectasis and subjects under TB exposure risk.

No MeSH data available.


Related in: MedlinePlus