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Evaluation of Anti-TBGL Antibody in the Diagnosis of Tuberculosis Patients in China.

Zhao J, Zhu Z, Zhang X, Suzuki Y, Chagan-Yasutan H, Chen H, Wan Y, Xu J, Ashino Y, Hattori T - J Immunol Res (2015)

Bottom Line: However, anti-TBGL IgA measurements were significantly less sensitive in detecting ETB than PTB (15.4% versus 46.7% sensitivity) but showed up to 89.7% specificity.Unlike anti-lipoarabinomannan (LAM) IgG that was found to elevate in sputum smearpositive subjects, anti-TBGL IgG and IgA elevated in those with cavitation and bronchiectasis, respectively.Anti-TBGL IgG in cavitary TB yielded 78.2% sensitivity compared to 57.1% in those otherwise.

View Article: PubMed Central - PubMed

Affiliation: Division of Emerging Infectious Diseases, Department of Internal Medicine, Graduate School of Medicine, Tohoku University, Sendai, Miyagi 980-8574, Japan.

ABSTRACT
Tuberculous glycolipid (TBGL) is a component of the Mycobacterium tuberculosis cell wall, and anti-TBGL antibodies are used for serodiagnosis of tuberculosis. Anti-TBGL IgG and IgA levels were measured in 45 pulmonary TB patients (PTB), 26 extra-pulmonary TB patients (ETB), 16 AIDS-TB patients, and 58 healthy controls (HC) including 39 health care workers (HW) and 19 newly enrolled students (ST). Anti-TBGL IgG measurements yielded 68.9% and 46.2% sensitivity in PTB and ETB, respectively, and 81.0% specificity. However, anti-TBGL IgA measurements were significantly less sensitive in detecting ETB than PTB (15.4% versus 46.7% sensitivity) but showed up to 89.7% specificity. Samples from AIDS-TB patients exhibited low reaction of anti-TBGL IgG and IgA with 6.3% and 12.5% sensitivity, respectively. Unlike anti-lipoarabinomannan (LAM) IgG that was found to elevate in sputum smearpositive subjects, anti-TBGL IgG and IgA elevated in those with cavitation and bronchiectasis, respectively. Anti-TBGL IgG in cavitary TB yielded 78.2% sensitivity compared to 57.1% in those otherwise. Meanwhile, higher anti-TBGL IgA titers were observed in HW than in ST, and increasing anti-TBGL IgG titers were observed in HW on follow-up. Therefore, higher anti-TBGL antibody titers are present in patients presenting cavities and bronchiectasis and subjects under TB exposure risk.

No MeSH data available.


Related in: MedlinePlus

ROC analysis for different antibodies. (a) and (b) ROC analysis between PTB patients and HC for anti-TBGL IgA and anti-LAM IgG, respectively; (c) ROC analysis between cavities positive and negative subjects for anti-TBGL IgG; (d) ROC analysis between bronchiectasis positive and negative subjects for anti-TBGL IgA. Circle: estimated optimal cutoff according to Youden's index; triangle: arbitrary cutoff.
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fig3: ROC analysis for different antibodies. (a) and (b) ROC analysis between PTB patients and HC for anti-TBGL IgA and anti-LAM IgG, respectively; (c) ROC analysis between cavities positive and negative subjects for anti-TBGL IgG; (d) ROC analysis between bronchiectasis positive and negative subjects for anti-TBGL IgA. Circle: estimated optimal cutoff according to Youden's index; triangle: arbitrary cutoff.

Mentions: The anti-LAM IgG ELISA method has been previously described [11]. ELISA Nunc MaxiSorp plates (Thermo Fisher Scientific, Inc., Waltham, MA) [12] were coated with 100 μL per well of 0.5 μg/mL purified lipoarabinomannan (LAM) (NACALAI TESQUE, INC.). A polyclonal antibody from Dr. Makoto Matsumoto (Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan), which was made from a LAM immunized rabbit, was used as a positive control. Serum samples were diluted 100-fold in fetal bovine serum and incubated in coated well for 1 hour. After being washed, HRP-conjugated goat anti-human IgG heavy chain polyclonal antibody (LifeSpan BioSciences, Inc., Seattle, WA) was diluted 1 : 10,000 in 1% (w/v) BSA in PBS and added as the secondary antibody to detect anti-LAM IgG. Reactions were visualized using a TMB HRP substrate kit (KPL, Inc., Gaithersburg, MD). Optical density (OD) values were measured at 450 nm. The cutoff for anti-LAM IgG was set based on Receiver Operating Characteristic (ROC) curve (Figure 3(c)).


Evaluation of Anti-TBGL Antibody in the Diagnosis of Tuberculosis Patients in China.

Zhao J, Zhu Z, Zhang X, Suzuki Y, Chagan-Yasutan H, Chen H, Wan Y, Xu J, Ashino Y, Hattori T - J Immunol Res (2015)

ROC analysis for different antibodies. (a) and (b) ROC analysis between PTB patients and HC for anti-TBGL IgA and anti-LAM IgG, respectively; (c) ROC analysis between cavities positive and negative subjects for anti-TBGL IgG; (d) ROC analysis between bronchiectasis positive and negative subjects for anti-TBGL IgA. Circle: estimated optimal cutoff according to Youden's index; triangle: arbitrary cutoff.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4539117&req=5

fig3: ROC analysis for different antibodies. (a) and (b) ROC analysis between PTB patients and HC for anti-TBGL IgA and anti-LAM IgG, respectively; (c) ROC analysis between cavities positive and negative subjects for anti-TBGL IgG; (d) ROC analysis between bronchiectasis positive and negative subjects for anti-TBGL IgA. Circle: estimated optimal cutoff according to Youden's index; triangle: arbitrary cutoff.
Mentions: The anti-LAM IgG ELISA method has been previously described [11]. ELISA Nunc MaxiSorp plates (Thermo Fisher Scientific, Inc., Waltham, MA) [12] were coated with 100 μL per well of 0.5 μg/mL purified lipoarabinomannan (LAM) (NACALAI TESQUE, INC.). A polyclonal antibody from Dr. Makoto Matsumoto (Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan), which was made from a LAM immunized rabbit, was used as a positive control. Serum samples were diluted 100-fold in fetal bovine serum and incubated in coated well for 1 hour. After being washed, HRP-conjugated goat anti-human IgG heavy chain polyclonal antibody (LifeSpan BioSciences, Inc., Seattle, WA) was diluted 1 : 10,000 in 1% (w/v) BSA in PBS and added as the secondary antibody to detect anti-LAM IgG. Reactions were visualized using a TMB HRP substrate kit (KPL, Inc., Gaithersburg, MD). Optical density (OD) values were measured at 450 nm. The cutoff for anti-LAM IgG was set based on Receiver Operating Characteristic (ROC) curve (Figure 3(c)).

Bottom Line: However, anti-TBGL IgA measurements were significantly less sensitive in detecting ETB than PTB (15.4% versus 46.7% sensitivity) but showed up to 89.7% specificity.Unlike anti-lipoarabinomannan (LAM) IgG that was found to elevate in sputum smearpositive subjects, anti-TBGL IgG and IgA elevated in those with cavitation and bronchiectasis, respectively.Anti-TBGL IgG in cavitary TB yielded 78.2% sensitivity compared to 57.1% in those otherwise.

View Article: PubMed Central - PubMed

Affiliation: Division of Emerging Infectious Diseases, Department of Internal Medicine, Graduate School of Medicine, Tohoku University, Sendai, Miyagi 980-8574, Japan.

ABSTRACT
Tuberculous glycolipid (TBGL) is a component of the Mycobacterium tuberculosis cell wall, and anti-TBGL antibodies are used for serodiagnosis of tuberculosis. Anti-TBGL IgG and IgA levels were measured in 45 pulmonary TB patients (PTB), 26 extra-pulmonary TB patients (ETB), 16 AIDS-TB patients, and 58 healthy controls (HC) including 39 health care workers (HW) and 19 newly enrolled students (ST). Anti-TBGL IgG measurements yielded 68.9% and 46.2% sensitivity in PTB and ETB, respectively, and 81.0% specificity. However, anti-TBGL IgA measurements were significantly less sensitive in detecting ETB than PTB (15.4% versus 46.7% sensitivity) but showed up to 89.7% specificity. Samples from AIDS-TB patients exhibited low reaction of anti-TBGL IgG and IgA with 6.3% and 12.5% sensitivity, respectively. Unlike anti-lipoarabinomannan (LAM) IgG that was found to elevate in sputum smearpositive subjects, anti-TBGL IgG and IgA elevated in those with cavitation and bronchiectasis, respectively. Anti-TBGL IgG in cavitary TB yielded 78.2% sensitivity compared to 57.1% in those otherwise. Meanwhile, higher anti-TBGL IgA titers were observed in HW than in ST, and increasing anti-TBGL IgG titers were observed in HW on follow-up. Therefore, higher anti-TBGL antibody titers are present in patients presenting cavities and bronchiectasis and subjects under TB exposure risk.

No MeSH data available.


Related in: MedlinePlus