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Evaluation of Anti-TBGL Antibody in the Diagnosis of Tuberculosis Patients in China.

Zhao J, Zhu Z, Zhang X, Suzuki Y, Chagan-Yasutan H, Chen H, Wan Y, Xu J, Ashino Y, Hattori T - J Immunol Res (2015)

Bottom Line: However, anti-TBGL IgA measurements were significantly less sensitive in detecting ETB than PTB (15.4% versus 46.7% sensitivity) but showed up to 89.7% specificity.Unlike anti-lipoarabinomannan (LAM) IgG that was found to elevate in sputum smearpositive subjects, anti-TBGL IgG and IgA elevated in those with cavitation and bronchiectasis, respectively.Anti-TBGL IgG in cavitary TB yielded 78.2% sensitivity compared to 57.1% in those otherwise.

View Article: PubMed Central - PubMed

Affiliation: Division of Emerging Infectious Diseases, Department of Internal Medicine, Graduate School of Medicine, Tohoku University, Sendai, Miyagi 980-8574, Japan.

ABSTRACT
Tuberculous glycolipid (TBGL) is a component of the Mycobacterium tuberculosis cell wall, and anti-TBGL antibodies are used for serodiagnosis of tuberculosis. Anti-TBGL IgG and IgA levels were measured in 45 pulmonary TB patients (PTB), 26 extra-pulmonary TB patients (ETB), 16 AIDS-TB patients, and 58 healthy controls (HC) including 39 health care workers (HW) and 19 newly enrolled students (ST). Anti-TBGL IgG measurements yielded 68.9% and 46.2% sensitivity in PTB and ETB, respectively, and 81.0% specificity. However, anti-TBGL IgA measurements were significantly less sensitive in detecting ETB than PTB (15.4% versus 46.7% sensitivity) but showed up to 89.7% specificity. Samples from AIDS-TB patients exhibited low reaction of anti-TBGL IgG and IgA with 6.3% and 12.5% sensitivity, respectively. Unlike anti-lipoarabinomannan (LAM) IgG that was found to elevate in sputum smearpositive subjects, anti-TBGL IgG and IgA elevated in those with cavitation and bronchiectasis, respectively. Anti-TBGL IgG in cavitary TB yielded 78.2% sensitivity compared to 57.1% in those otherwise. Meanwhile, higher anti-TBGL IgA titers were observed in HW than in ST, and increasing anti-TBGL IgG titers were observed in HW on follow-up. Therefore, higher anti-TBGL antibody titers are present in patients presenting cavities and bronchiectasis and subjects under TB exposure risk.

No MeSH data available.


Related in: MedlinePlus

Elevated titers in association with different clinical findings. (a), (b), and (c) display findings of PTB-cavity versus PTB-noncavity, PTB-bronchiectasis versus PTB-nonbronchiectasis, and PTB-smear-negative versus PTB-smear-positive, respectively. A p value less than 0.05 indicates a significant difference between 2 groups by nonparametric t-test. (L), (M), and (R) referred to anti-TBGL IgG, anti-TBGL IgA, and anti-LAM IgG, respectively.
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fig2: Elevated titers in association with different clinical findings. (a), (b), and (c) display findings of PTB-cavity versus PTB-noncavity, PTB-bronchiectasis versus PTB-nonbronchiectasis, and PTB-smear-negative versus PTB-smear-positive, respectively. A p value less than 0.05 indicates a significant difference between 2 groups by nonparametric t-test. (L), (M), and (R) referred to anti-TBGL IgG, anti-TBGL IgA, and anti-LAM IgG, respectively.

Mentions: PTB patients were grouped in correspondence with the clinical findings listed in Table 1. Anti-TBGL IgG titers were significantly higher in patients with cavitation compared to those without such pathology, and significantly elevated anti-TBGL IgA titers were observed in subjects with bronchiectasis compared to those without it (t-test, p < 0.05, Figures 2(a) and 2(b)). However, there were no differences between subgroups for anti-LAM IgG with respect to chest radiographic findings (p > 0.05, Figure 2(c)), in spite of the fact that there were subjects with positive sputum smears that were higher anti-LAM IgG responses, which was not observed for anti-TBGL IgG or IgA (Figure 2(c)). Similar to PTB patients, ETB patients showed relatively high anti-TBGL IgG titers in contrast to anti-TBGL IgA titers which were lower in ETB samples. Remarkably, all 3 intestinal TB subjects were anti-TBGL IgG positive (Median 15.9 Range [3.9–27.9]), while only 2 out of 7 TB pleurisy subjects showed positive in anti-TBGL IgG (Median 1.2 Range [0.4–7.0]). Samples from AIDS-TB patients had considerably lower titers for anti-TBGL antibodies compared to other TB groups (Figures 1(a) and 1(b)).


Evaluation of Anti-TBGL Antibody in the Diagnosis of Tuberculosis Patients in China.

Zhao J, Zhu Z, Zhang X, Suzuki Y, Chagan-Yasutan H, Chen H, Wan Y, Xu J, Ashino Y, Hattori T - J Immunol Res (2015)

Elevated titers in association with different clinical findings. (a), (b), and (c) display findings of PTB-cavity versus PTB-noncavity, PTB-bronchiectasis versus PTB-nonbronchiectasis, and PTB-smear-negative versus PTB-smear-positive, respectively. A p value less than 0.05 indicates a significant difference between 2 groups by nonparametric t-test. (L), (M), and (R) referred to anti-TBGL IgG, anti-TBGL IgA, and anti-LAM IgG, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4539117&req=5

fig2: Elevated titers in association with different clinical findings. (a), (b), and (c) display findings of PTB-cavity versus PTB-noncavity, PTB-bronchiectasis versus PTB-nonbronchiectasis, and PTB-smear-negative versus PTB-smear-positive, respectively. A p value less than 0.05 indicates a significant difference between 2 groups by nonparametric t-test. (L), (M), and (R) referred to anti-TBGL IgG, anti-TBGL IgA, and anti-LAM IgG, respectively.
Mentions: PTB patients were grouped in correspondence with the clinical findings listed in Table 1. Anti-TBGL IgG titers were significantly higher in patients with cavitation compared to those without such pathology, and significantly elevated anti-TBGL IgA titers were observed in subjects with bronchiectasis compared to those without it (t-test, p < 0.05, Figures 2(a) and 2(b)). However, there were no differences between subgroups for anti-LAM IgG with respect to chest radiographic findings (p > 0.05, Figure 2(c)), in spite of the fact that there were subjects with positive sputum smears that were higher anti-LAM IgG responses, which was not observed for anti-TBGL IgG or IgA (Figure 2(c)). Similar to PTB patients, ETB patients showed relatively high anti-TBGL IgG titers in contrast to anti-TBGL IgA titers which were lower in ETB samples. Remarkably, all 3 intestinal TB subjects were anti-TBGL IgG positive (Median 15.9 Range [3.9–27.9]), while only 2 out of 7 TB pleurisy subjects showed positive in anti-TBGL IgG (Median 1.2 Range [0.4–7.0]). Samples from AIDS-TB patients had considerably lower titers for anti-TBGL antibodies compared to other TB groups (Figures 1(a) and 1(b)).

Bottom Line: However, anti-TBGL IgA measurements were significantly less sensitive in detecting ETB than PTB (15.4% versus 46.7% sensitivity) but showed up to 89.7% specificity.Unlike anti-lipoarabinomannan (LAM) IgG that was found to elevate in sputum smearpositive subjects, anti-TBGL IgG and IgA elevated in those with cavitation and bronchiectasis, respectively.Anti-TBGL IgG in cavitary TB yielded 78.2% sensitivity compared to 57.1% in those otherwise.

View Article: PubMed Central - PubMed

Affiliation: Division of Emerging Infectious Diseases, Department of Internal Medicine, Graduate School of Medicine, Tohoku University, Sendai, Miyagi 980-8574, Japan.

ABSTRACT
Tuberculous glycolipid (TBGL) is a component of the Mycobacterium tuberculosis cell wall, and anti-TBGL antibodies are used for serodiagnosis of tuberculosis. Anti-TBGL IgG and IgA levels were measured in 45 pulmonary TB patients (PTB), 26 extra-pulmonary TB patients (ETB), 16 AIDS-TB patients, and 58 healthy controls (HC) including 39 health care workers (HW) and 19 newly enrolled students (ST). Anti-TBGL IgG measurements yielded 68.9% and 46.2% sensitivity in PTB and ETB, respectively, and 81.0% specificity. However, anti-TBGL IgA measurements were significantly less sensitive in detecting ETB than PTB (15.4% versus 46.7% sensitivity) but showed up to 89.7% specificity. Samples from AIDS-TB patients exhibited low reaction of anti-TBGL IgG and IgA with 6.3% and 12.5% sensitivity, respectively. Unlike anti-lipoarabinomannan (LAM) IgG that was found to elevate in sputum smearpositive subjects, anti-TBGL IgG and IgA elevated in those with cavitation and bronchiectasis, respectively. Anti-TBGL IgG in cavitary TB yielded 78.2% sensitivity compared to 57.1% in those otherwise. Meanwhile, higher anti-TBGL IgA titers were observed in HW than in ST, and increasing anti-TBGL IgG titers were observed in HW on follow-up. Therefore, higher anti-TBGL antibody titers are present in patients presenting cavities and bronchiectasis and subjects under TB exposure risk.

No MeSH data available.


Related in: MedlinePlus