Limits...
Evaluation of Anti-TBGL Antibody in the Diagnosis of Tuberculosis Patients in China.

Zhao J, Zhu Z, Zhang X, Suzuki Y, Chagan-Yasutan H, Chen H, Wan Y, Xu J, Ashino Y, Hattori T - J Immunol Res (2015)

Bottom Line: However, anti-TBGL IgA measurements were significantly less sensitive in detecting ETB than PTB (15.4% versus 46.7% sensitivity) but showed up to 89.7% specificity.Unlike anti-lipoarabinomannan (LAM) IgG that was found to elevate in sputum smearpositive subjects, anti-TBGL IgG and IgA elevated in those with cavitation and bronchiectasis, respectively.Anti-TBGL IgG in cavitary TB yielded 78.2% sensitivity compared to 57.1% in those otherwise.

View Article: PubMed Central - PubMed

Affiliation: Division of Emerging Infectious Diseases, Department of Internal Medicine, Graduate School of Medicine, Tohoku University, Sendai, Miyagi 980-8574, Japan.

ABSTRACT
Tuberculous glycolipid (TBGL) is a component of the Mycobacterium tuberculosis cell wall, and anti-TBGL antibodies are used for serodiagnosis of tuberculosis. Anti-TBGL IgG and IgA levels were measured in 45 pulmonary TB patients (PTB), 26 extra-pulmonary TB patients (ETB), 16 AIDS-TB patients, and 58 healthy controls (HC) including 39 health care workers (HW) and 19 newly enrolled students (ST). Anti-TBGL IgG measurements yielded 68.9% and 46.2% sensitivity in PTB and ETB, respectively, and 81.0% specificity. However, anti-TBGL IgA measurements were significantly less sensitive in detecting ETB than PTB (15.4% versus 46.7% sensitivity) but showed up to 89.7% specificity. Samples from AIDS-TB patients exhibited low reaction of anti-TBGL IgG and IgA with 6.3% and 12.5% sensitivity, respectively. Unlike anti-lipoarabinomannan (LAM) IgG that was found to elevate in sputum smearpositive subjects, anti-TBGL IgG and IgA elevated in those with cavitation and bronchiectasis, respectively. Anti-TBGL IgG in cavitary TB yielded 78.2% sensitivity compared to 57.1% in those otherwise. Meanwhile, higher anti-TBGL IgA titers were observed in HW than in ST, and increasing anti-TBGL IgG titers were observed in HW on follow-up. Therefore, higher anti-TBGL antibody titers are present in patients presenting cavities and bronchiectasis and subjects under TB exposure risk.

No MeSH data available.


Related in: MedlinePlus

Detection of anti-TBGL IgG, anti-TBGL IgA, and anti-LAM IgG in TB patients and HC. PTB = pulmonary TB; ETB = extrapulmonary TB; HC = healthy control. Anti-TBGL antibody titers were evaluated in U/mL, and the anti-LAM IgG reactivity was assessed as OD values.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4539117&req=5

fig1: Detection of anti-TBGL IgG, anti-TBGL IgA, and anti-LAM IgG in TB patients and HC. PTB = pulmonary TB; ETB = extrapulmonary TB; HC = healthy control. Anti-TBGL antibody titers were evaluated in U/mL, and the anti-LAM IgG reactivity was assessed as OD values.

Mentions: Plasma levels of anti-TBGL IgG and IgA were measured using a Determiner TBGL Antibody ELISA kit (Kyowa Medex Co., Ltd., Tokyo, Japan). This assay uses TBGL antigen which is a combination of trehalose 6,6-dimycolate (TDM) and hydrophobic glycolipids extracted from MTB H37Rv and has been described previously [7]. The cutoff index for anti-TBGL IgG and IgA was set to 2.0 U/mL in accordance with previous studies (Figure 1(a)) [4, 5].


Evaluation of Anti-TBGL Antibody in the Diagnosis of Tuberculosis Patients in China.

Zhao J, Zhu Z, Zhang X, Suzuki Y, Chagan-Yasutan H, Chen H, Wan Y, Xu J, Ashino Y, Hattori T - J Immunol Res (2015)

Detection of anti-TBGL IgG, anti-TBGL IgA, and anti-LAM IgG in TB patients and HC. PTB = pulmonary TB; ETB = extrapulmonary TB; HC = healthy control. Anti-TBGL antibody titers were evaluated in U/mL, and the anti-LAM IgG reactivity was assessed as OD values.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4539117&req=5

fig1: Detection of anti-TBGL IgG, anti-TBGL IgA, and anti-LAM IgG in TB patients and HC. PTB = pulmonary TB; ETB = extrapulmonary TB; HC = healthy control. Anti-TBGL antibody titers were evaluated in U/mL, and the anti-LAM IgG reactivity was assessed as OD values.
Mentions: Plasma levels of anti-TBGL IgG and IgA were measured using a Determiner TBGL Antibody ELISA kit (Kyowa Medex Co., Ltd., Tokyo, Japan). This assay uses TBGL antigen which is a combination of trehalose 6,6-dimycolate (TDM) and hydrophobic glycolipids extracted from MTB H37Rv and has been described previously [7]. The cutoff index for anti-TBGL IgG and IgA was set to 2.0 U/mL in accordance with previous studies (Figure 1(a)) [4, 5].

Bottom Line: However, anti-TBGL IgA measurements were significantly less sensitive in detecting ETB than PTB (15.4% versus 46.7% sensitivity) but showed up to 89.7% specificity.Unlike anti-lipoarabinomannan (LAM) IgG that was found to elevate in sputum smearpositive subjects, anti-TBGL IgG and IgA elevated in those with cavitation and bronchiectasis, respectively.Anti-TBGL IgG in cavitary TB yielded 78.2% sensitivity compared to 57.1% in those otherwise.

View Article: PubMed Central - PubMed

Affiliation: Division of Emerging Infectious Diseases, Department of Internal Medicine, Graduate School of Medicine, Tohoku University, Sendai, Miyagi 980-8574, Japan.

ABSTRACT
Tuberculous glycolipid (TBGL) is a component of the Mycobacterium tuberculosis cell wall, and anti-TBGL antibodies are used for serodiagnosis of tuberculosis. Anti-TBGL IgG and IgA levels were measured in 45 pulmonary TB patients (PTB), 26 extra-pulmonary TB patients (ETB), 16 AIDS-TB patients, and 58 healthy controls (HC) including 39 health care workers (HW) and 19 newly enrolled students (ST). Anti-TBGL IgG measurements yielded 68.9% and 46.2% sensitivity in PTB and ETB, respectively, and 81.0% specificity. However, anti-TBGL IgA measurements were significantly less sensitive in detecting ETB than PTB (15.4% versus 46.7% sensitivity) but showed up to 89.7% specificity. Samples from AIDS-TB patients exhibited low reaction of anti-TBGL IgG and IgA with 6.3% and 12.5% sensitivity, respectively. Unlike anti-lipoarabinomannan (LAM) IgG that was found to elevate in sputum smearpositive subjects, anti-TBGL IgG and IgA elevated in those with cavitation and bronchiectasis, respectively. Anti-TBGL IgG in cavitary TB yielded 78.2% sensitivity compared to 57.1% in those otherwise. Meanwhile, higher anti-TBGL IgA titers were observed in HW than in ST, and increasing anti-TBGL IgG titers were observed in HW on follow-up. Therefore, higher anti-TBGL antibody titers are present in patients presenting cavities and bronchiectasis and subjects under TB exposure risk.

No MeSH data available.


Related in: MedlinePlus