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Progranulin Is Associated with Disease Activity in Patients with Rheumatoid Arthritis.

Cerezo LA, Kuklová M, Hulejová H, Vernerová Z, Kaspříková N, Veigl D, Pavelka K, Vencovský J, Šenolt L - Mediators Inflamm. (2015)

Bottom Line: Synovial fluid levels of PGRN were higher in patients with RA compared to OA (384.5 ± 275.3 versus 241.4 ± 165.2 ng/mL; P = 0.002).PGRN expression was significantly upregulated in the synovial tissue of RA patients particularly in the inflammatory infiltrates.PGRN levels were not associated with CRP or autoantibodies.

View Article: PubMed Central - PubMed

Affiliation: Institute of Rheumatology, Na Slupi 4, 12850 Prague 2, Czech Republic.

ABSTRACT

Objective: Progranulin (PGRN) is implicated in the pathogenesis of rheumatoid arthritis (RA). The aim of this study was to assess the relationship between PGRN and disease activity in RA.

Methods: PGRN levels were evaluated in patients with RA (n = 47) and OA (n = 42) and healthy controls (n = 41). Immunohistochemical analysis of PGRN in synovial tissues was performed. The association between PGRN and C-reactive protein (CRP), disease activity score (DAS28-CRP), and health assessment questionnaire (HAQ) was studied.

Results: Circulating PGRN was elevated in patients with RA and OA compared to healthy controls (227.1 ± 100.2 and 221.5 ± 102.5 versus 128.1 ± 34.7 ng/mL; P < 0.001). Synovial fluid levels of PGRN were higher in patients with RA compared to OA (384.5 ± 275.3 versus 241.4 ± 165.2 ng/mL; P = 0.002). PGRN expression was significantly upregulated in the synovial tissue of RA patients particularly in the inflammatory infiltrates. Serum PGRN levels correlated with DAS28 (r = 0.327, P = 0.049) and HAQ score (r = 0.323, P = 0.032), while synovial fluid PGRN correlated only with HAQ (r = 0.310, P = 0.043) in patients with RA. PGRN levels were not associated with CRP or autoantibodies.

Conclusions: This study demonstrates increased PGRN expression at local sites of inflammation and association between PGRN levels, disease activity, and functional impairment in patients with RA.

No MeSH data available.


Related in: MedlinePlus

Detection of progranulin (PGRN) protein in rheumatoid arthritis (RA) synovial tissues (a1) and osteoarthritis synovial tissues (b1). Strong staining intensity for PGRN was observed in the lining layer and particularly in the mononuclear cell infiltrates of the sublining layer of RA synovial tissue. Vessels and capillaries were positive for PGRN in both RA and OA synovial tissues. Mouse IgG was used as an isotype control (a2, b2). The original magnification is 200x.
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Related In: Results  -  Collection


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fig1: Detection of progranulin (PGRN) protein in rheumatoid arthritis (RA) synovial tissues (a1) and osteoarthritis synovial tissues (b1). Strong staining intensity for PGRN was observed in the lining layer and particularly in the mononuclear cell infiltrates of the sublining layer of RA synovial tissue. Vessels and capillaries were positive for PGRN in both RA and OA synovial tissues. Mouse IgG was used as an isotype control (a2, b2). The original magnification is 200x.

Mentions: The expression of PGRN was detected in both RA and OA synovial tissues; however, the staining intensity was significantly enhanced in patients with RA (Figure 1). In comparison with OA samples, PGRN was significantly upregulated in the inflammatory cells localized in synovial sublining layer of patients with RA (P = 0.033). The synovial lining layer showed slightly higher expression of PGRN in RA compared to OA tissue samples (P = 0.086). A comparable PGRN staining intensity was detected in the vessels between the RA and OA synovial tissues (P = 0.462) (Table 2).


Progranulin Is Associated with Disease Activity in Patients with Rheumatoid Arthritis.

Cerezo LA, Kuklová M, Hulejová H, Vernerová Z, Kaspříková N, Veigl D, Pavelka K, Vencovský J, Šenolt L - Mediators Inflamm. (2015)

Detection of progranulin (PGRN) protein in rheumatoid arthritis (RA) synovial tissues (a1) and osteoarthritis synovial tissues (b1). Strong staining intensity for PGRN was observed in the lining layer and particularly in the mononuclear cell infiltrates of the sublining layer of RA synovial tissue. Vessels and capillaries were positive for PGRN in both RA and OA synovial tissues. Mouse IgG was used as an isotype control (a2, b2). The original magnification is 200x.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4539115&req=5

fig1: Detection of progranulin (PGRN) protein in rheumatoid arthritis (RA) synovial tissues (a1) and osteoarthritis synovial tissues (b1). Strong staining intensity for PGRN was observed in the lining layer and particularly in the mononuclear cell infiltrates of the sublining layer of RA synovial tissue. Vessels and capillaries were positive for PGRN in both RA and OA synovial tissues. Mouse IgG was used as an isotype control (a2, b2). The original magnification is 200x.
Mentions: The expression of PGRN was detected in both RA and OA synovial tissues; however, the staining intensity was significantly enhanced in patients with RA (Figure 1). In comparison with OA samples, PGRN was significantly upregulated in the inflammatory cells localized in synovial sublining layer of patients with RA (P = 0.033). The synovial lining layer showed slightly higher expression of PGRN in RA compared to OA tissue samples (P = 0.086). A comparable PGRN staining intensity was detected in the vessels between the RA and OA synovial tissues (P = 0.462) (Table 2).

Bottom Line: Synovial fluid levels of PGRN were higher in patients with RA compared to OA (384.5 ± 275.3 versus 241.4 ± 165.2 ng/mL; P = 0.002).PGRN expression was significantly upregulated in the synovial tissue of RA patients particularly in the inflammatory infiltrates.PGRN levels were not associated with CRP or autoantibodies.

View Article: PubMed Central - PubMed

Affiliation: Institute of Rheumatology, Na Slupi 4, 12850 Prague 2, Czech Republic.

ABSTRACT

Objective: Progranulin (PGRN) is implicated in the pathogenesis of rheumatoid arthritis (RA). The aim of this study was to assess the relationship between PGRN and disease activity in RA.

Methods: PGRN levels were evaluated in patients with RA (n = 47) and OA (n = 42) and healthy controls (n = 41). Immunohistochemical analysis of PGRN in synovial tissues was performed. The association between PGRN and C-reactive protein (CRP), disease activity score (DAS28-CRP), and health assessment questionnaire (HAQ) was studied.

Results: Circulating PGRN was elevated in patients with RA and OA compared to healthy controls (227.1 ± 100.2 and 221.5 ± 102.5 versus 128.1 ± 34.7 ng/mL; P < 0.001). Synovial fluid levels of PGRN were higher in patients with RA compared to OA (384.5 ± 275.3 versus 241.4 ± 165.2 ng/mL; P = 0.002). PGRN expression was significantly upregulated in the synovial tissue of RA patients particularly in the inflammatory infiltrates. Serum PGRN levels correlated with DAS28 (r = 0.327, P = 0.049) and HAQ score (r = 0.323, P = 0.032), while synovial fluid PGRN correlated only with HAQ (r = 0.310, P = 0.043) in patients with RA. PGRN levels were not associated with CRP or autoantibodies.

Conclusions: This study demonstrates increased PGRN expression at local sites of inflammation and association between PGRN levels, disease activity, and functional impairment in patients with RA.

No MeSH data available.


Related in: MedlinePlus