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Autoantibody Profiles in Collagen Disease Patients with Interstitial Lung Disease (ILD): Antibodies to Major Histocompatibility Complex Class I-Related Chain A (MICA) as Markers of ILD.

Furukawa H, Oka S, Shimada K, Masuo K, Nakajima F, Funano S, Tanaka Y, Komiya A, Fukui N, Sawasaki T, Tadokoro K, Nose M, Tsuchiya N, Tohma S - Biomark Insights (2015)

Bottom Line: It is then designated as collagen vascular disease-associated ILD (CVD-ILD), and influences patients' prognosis.The ratio of the average anti-MICA Ab level to the average anti-human leukocyte antigen class I Ab level (ie, MICA/Class I) was significantly higher in RA patients with CVD-ILD compared with those without (P = 4.47 × 10(-5)).The MICA/Class I ratio could be a better marker for diagnosing CVD-ILD than KL-6 (Krebs von den lungen-6).

View Article: PubMed Central - PubMed

Affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara Hospital, National Hospital Organization, Sagamihara, Japan. ; Molecular and Genetic Epidemiology Laboratory, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.

ABSTRACT
Interstitial lung disease (ILD) is frequently associated with collagen disease. It is then designated as collagen vascular disease-associated ILD (CVD-ILD), and influences patients' prognosis. The prognosis of acute-onset diffuse ILD (AoDILD) occurring in patients with collagen disease is quite poor. Here, we report our investigation of auto-antibody (Ab) profiles to determine whether they may be useful in diagnosing CVD-ILD or AoDILD in collagen disease. Auto-Ab profiles were analyzed using the Lambda Array Beads Multi-Analyte System, granulocyte immunofluorescence test, Proto-Array Human Protein Microarray, AlphaScreen assay, and glutathione S-transferase capture enzyme-linked immunosorbent assay in 34 patients with rheumatoid arthritis (RA) with or without CVD-ILD and in 15 patients with collagen disease with AoDILD. The average anti-major histocompatibility complex class I-related chain A (MICA) Ab levels were higher in RA patients with CVD-ILD than in those without (P = 0.0013). The ratio of the average anti-MICA Ab level to the average anti-human leukocyte antigen class I Ab level (ie, MICA/Class I) was significantly higher in RA patients with CVD-ILD compared with those without (P = 4.47 × 10(-5)). To the best of our knowledge, this is the first report of auto-Ab profiles in CVD-ILD. The MICA/Class I ratio could be a better marker for diagnosing CVD-ILD than KL-6 (Krebs von den lungen-6).

No MeSH data available.


Related in: MedlinePlus

Auto-Abs present in the pooled sera from collagen disease patients with acute-onset diffuse ILD (AoDILD). The numbers of auto-Abs detected with (A) ProtoArray and (b) AlphaScreen in pooled sera from patients with collagen disease in the stable (left circle) and AoDILD states (right circle). The numbers of auto-Abs for proteins overlapped between (C) ProtoArray and (D) AlphaScreen in pooled sera from patients with collagen disease in the stable (left circle) and AoDILD states (right circle).
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f4-bmi-10-2015-063: Auto-Abs present in the pooled sera from collagen disease patients with acute-onset diffuse ILD (AoDILD). The numbers of auto-Abs detected with (A) ProtoArray and (b) AlphaScreen in pooled sera from patients with collagen disease in the stable (left circle) and AoDILD states (right circle). The numbers of auto-Abs for proteins overlapped between (C) ProtoArray and (D) AlphaScreen in pooled sera from patients with collagen disease in the stable (left circle) and AoDILD states (right circle).

Mentions: Auto-Ab levels in pooled sera were compared between the AoDILD and stable states with ProtoArray (Fig. 4A, Supplementary Table S2). According to the Z-factor cutoff value of 0.4, auto-Abs for 767 probes (689 proteins) were present in both the AoDILD and stable states. Auto-Abs for a further 865 probes (827 proteins) were found in the AoDILD but not in the stable state. Reciprocally, auto-Ab for 82 probes (80 proteins) were found in the stable but not in the AoDILD state. For the remaining 7,769 probes (6,566 proteins), there were no auto-Abs detected in either state. The number of auto-Abs present only in the sera from patients with AoDILD was greater than the number of auto-Abs only present in the stable state. Anti-HLA-B or C Abs were detected neither in the AoDILD nor stable states with ProtoArray. Thus, auto-Ab profiles were different in AoDILD and stable states.


Autoantibody Profiles in Collagen Disease Patients with Interstitial Lung Disease (ILD): Antibodies to Major Histocompatibility Complex Class I-Related Chain A (MICA) as Markers of ILD.

Furukawa H, Oka S, Shimada K, Masuo K, Nakajima F, Funano S, Tanaka Y, Komiya A, Fukui N, Sawasaki T, Tadokoro K, Nose M, Tsuchiya N, Tohma S - Biomark Insights (2015)

Auto-Abs present in the pooled sera from collagen disease patients with acute-onset diffuse ILD (AoDILD). The numbers of auto-Abs detected with (A) ProtoArray and (b) AlphaScreen in pooled sera from patients with collagen disease in the stable (left circle) and AoDILD states (right circle). The numbers of auto-Abs for proteins overlapped between (C) ProtoArray and (D) AlphaScreen in pooled sera from patients with collagen disease in the stable (left circle) and AoDILD states (right circle).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4539100&req=5

f4-bmi-10-2015-063: Auto-Abs present in the pooled sera from collagen disease patients with acute-onset diffuse ILD (AoDILD). The numbers of auto-Abs detected with (A) ProtoArray and (b) AlphaScreen in pooled sera from patients with collagen disease in the stable (left circle) and AoDILD states (right circle). The numbers of auto-Abs for proteins overlapped between (C) ProtoArray and (D) AlphaScreen in pooled sera from patients with collagen disease in the stable (left circle) and AoDILD states (right circle).
Mentions: Auto-Ab levels in pooled sera were compared between the AoDILD and stable states with ProtoArray (Fig. 4A, Supplementary Table S2). According to the Z-factor cutoff value of 0.4, auto-Abs for 767 probes (689 proteins) were present in both the AoDILD and stable states. Auto-Abs for a further 865 probes (827 proteins) were found in the AoDILD but not in the stable state. Reciprocally, auto-Ab for 82 probes (80 proteins) were found in the stable but not in the AoDILD state. For the remaining 7,769 probes (6,566 proteins), there were no auto-Abs detected in either state. The number of auto-Abs present only in the sera from patients with AoDILD was greater than the number of auto-Abs only present in the stable state. Anti-HLA-B or C Abs were detected neither in the AoDILD nor stable states with ProtoArray. Thus, auto-Ab profiles were different in AoDILD and stable states.

Bottom Line: It is then designated as collagen vascular disease-associated ILD (CVD-ILD), and influences patients' prognosis.The ratio of the average anti-MICA Ab level to the average anti-human leukocyte antigen class I Ab level (ie, MICA/Class I) was significantly higher in RA patients with CVD-ILD compared with those without (P = 4.47 × 10(-5)).The MICA/Class I ratio could be a better marker for diagnosing CVD-ILD than KL-6 (Krebs von den lungen-6).

View Article: PubMed Central - PubMed

Affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara Hospital, National Hospital Organization, Sagamihara, Japan. ; Molecular and Genetic Epidemiology Laboratory, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.

ABSTRACT
Interstitial lung disease (ILD) is frequently associated with collagen disease. It is then designated as collagen vascular disease-associated ILD (CVD-ILD), and influences patients' prognosis. The prognosis of acute-onset diffuse ILD (AoDILD) occurring in patients with collagen disease is quite poor. Here, we report our investigation of auto-antibody (Ab) profiles to determine whether they may be useful in diagnosing CVD-ILD or AoDILD in collagen disease. Auto-Ab profiles were analyzed using the Lambda Array Beads Multi-Analyte System, granulocyte immunofluorescence test, Proto-Array Human Protein Microarray, AlphaScreen assay, and glutathione S-transferase capture enzyme-linked immunosorbent assay in 34 patients with rheumatoid arthritis (RA) with or without CVD-ILD and in 15 patients with collagen disease with AoDILD. The average anti-major histocompatibility complex class I-related chain A (MICA) Ab levels were higher in RA patients with CVD-ILD than in those without (P = 0.0013). The ratio of the average anti-MICA Ab level to the average anti-human leukocyte antigen class I Ab level (ie, MICA/Class I) was significantly higher in RA patients with CVD-ILD compared with those without (P = 4.47 × 10(-5)). To the best of our knowledge, this is the first report of auto-Ab profiles in CVD-ILD. The MICA/Class I ratio could be a better marker for diagnosing CVD-ILD than KL-6 (Krebs von den lungen-6).

No MeSH data available.


Related in: MedlinePlus