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Autoantibody Profiles in Collagen Disease Patients with Interstitial Lung Disease (ILD): Antibodies to Major Histocompatibility Complex Class I-Related Chain A (MICA) as Markers of ILD.

Furukawa H, Oka S, Shimada K, Masuo K, Nakajima F, Funano S, Tanaka Y, Komiya A, Fukui N, Sawasaki T, Tadokoro K, Nose M, Tsuchiya N, Tohma S - Biomark Insights (2015)

Bottom Line: It is then designated as collagen vascular disease-associated ILD (CVD-ILD), and influences patients' prognosis.The ratio of the average anti-MICA Ab level to the average anti-human leukocyte antigen class I Ab level (ie, MICA/Class I) was significantly higher in RA patients with CVD-ILD compared with those without (P = 4.47 × 10(-5)).The MICA/Class I ratio could be a better marker for diagnosing CVD-ILD than KL-6 (Krebs von den lungen-6).

View Article: PubMed Central - PubMed

Affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara Hospital, National Hospital Organization, Sagamihara, Japan. ; Molecular and Genetic Epidemiology Laboratory, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.

ABSTRACT
Interstitial lung disease (ILD) is frequently associated with collagen disease. It is then designated as collagen vascular disease-associated ILD (CVD-ILD), and influences patients' prognosis. The prognosis of acute-onset diffuse ILD (AoDILD) occurring in patients with collagen disease is quite poor. Here, we report our investigation of auto-antibody (Ab) profiles to determine whether they may be useful in diagnosing CVD-ILD or AoDILD in collagen disease. Auto-Ab profiles were analyzed using the Lambda Array Beads Multi-Analyte System, granulocyte immunofluorescence test, Proto-Array Human Protein Microarray, AlphaScreen assay, and glutathione S-transferase capture enzyme-linked immunosorbent assay in 34 patients with rheumatoid arthritis (RA) with or without CVD-ILD and in 15 patients with collagen disease with AoDILD. The average anti-major histocompatibility complex class I-related chain A (MICA) Ab levels were higher in RA patients with CVD-ILD than in those without (P = 0.0013). The ratio of the average anti-MICA Ab level to the average anti-human leukocyte antigen class I Ab level (ie, MICA/Class I) was significantly higher in RA patients with CVD-ILD compared with those without (P = 4.47 × 10(-5)). To the best of our knowledge, this is the first report of auto-Ab profiles in CVD-ILD. The MICA/Class I ratio could be a better marker for diagnosing CVD-ILD than KL-6 (Krebs von den lungen-6).

No MeSH data available.


Related in: MedlinePlus

Auto-Ab identification methods used in this study. (A) Sera from the 34 rheumatoid arthritis (RA) patients with or without ILD were analyzed for anti-HLA Ab profiles. (b) Sera from the 15 collagen disease patients with acute-onset diffuse ILD (AoDILD) were collected on admission and in the stable state. Two samples were collected from each patient. These individual sera were analyzed for anti-HLA Ab profiles or granulocyte immunofluorescence test (GIFT). (C) The sera from these patients either with AoDILD or in the stable state were combined; the two pooled sera at these two states were screened for the auto-Ab profiling in ProtoArray and AlphaScreen systems. (D) These individual sera were analyzed by glutathione S-transferase (GST) capture enzyme-linked immunosorbent assay (ELISA). Analyses of individual sera are written in black and those of pooled sera in red.
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f1-bmi-10-2015-063: Auto-Ab identification methods used in this study. (A) Sera from the 34 rheumatoid arthritis (RA) patients with or without ILD were analyzed for anti-HLA Ab profiles. (b) Sera from the 15 collagen disease patients with acute-onset diffuse ILD (AoDILD) were collected on admission and in the stable state. Two samples were collected from each patient. These individual sera were analyzed for anti-HLA Ab profiles or granulocyte immunofluorescence test (GIFT). (C) The sera from these patients either with AoDILD or in the stable state were combined; the two pooled sera at these two states were screened for the auto-Ab profiling in ProtoArray and AlphaScreen systems. (D) These individual sera were analyzed by glutathione S-transferase (GST) capture enzyme-linked immunosorbent assay (ELISA). Analyses of individual sera are written in black and those of pooled sera in red.

Mentions: Sera from the 15 collagen disease patients with AoDILD were collected on admission and in the stable state, at least three months before admission. Two samples were collected from each patient. These individual sera were analyzed for anti-HLA Ab profiles. The sera from these patients either with AoDILD or in the stable state were combined; the two pooled sera at these two states were screened for the auto-Ab profiling to be described in the following sections (Fig. 1).


Autoantibody Profiles in Collagen Disease Patients with Interstitial Lung Disease (ILD): Antibodies to Major Histocompatibility Complex Class I-Related Chain A (MICA) as Markers of ILD.

Furukawa H, Oka S, Shimada K, Masuo K, Nakajima F, Funano S, Tanaka Y, Komiya A, Fukui N, Sawasaki T, Tadokoro K, Nose M, Tsuchiya N, Tohma S - Biomark Insights (2015)

Auto-Ab identification methods used in this study. (A) Sera from the 34 rheumatoid arthritis (RA) patients with or without ILD were analyzed for anti-HLA Ab profiles. (b) Sera from the 15 collagen disease patients with acute-onset diffuse ILD (AoDILD) were collected on admission and in the stable state. Two samples were collected from each patient. These individual sera were analyzed for anti-HLA Ab profiles or granulocyte immunofluorescence test (GIFT). (C) The sera from these patients either with AoDILD or in the stable state were combined; the two pooled sera at these two states were screened for the auto-Ab profiling in ProtoArray and AlphaScreen systems. (D) These individual sera were analyzed by glutathione S-transferase (GST) capture enzyme-linked immunosorbent assay (ELISA). Analyses of individual sera are written in black and those of pooled sera in red.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4539100&req=5

f1-bmi-10-2015-063: Auto-Ab identification methods used in this study. (A) Sera from the 34 rheumatoid arthritis (RA) patients with or without ILD were analyzed for anti-HLA Ab profiles. (b) Sera from the 15 collagen disease patients with acute-onset diffuse ILD (AoDILD) were collected on admission and in the stable state. Two samples were collected from each patient. These individual sera were analyzed for anti-HLA Ab profiles or granulocyte immunofluorescence test (GIFT). (C) The sera from these patients either with AoDILD or in the stable state were combined; the two pooled sera at these two states were screened for the auto-Ab profiling in ProtoArray and AlphaScreen systems. (D) These individual sera were analyzed by glutathione S-transferase (GST) capture enzyme-linked immunosorbent assay (ELISA). Analyses of individual sera are written in black and those of pooled sera in red.
Mentions: Sera from the 15 collagen disease patients with AoDILD were collected on admission and in the stable state, at least three months before admission. Two samples were collected from each patient. These individual sera were analyzed for anti-HLA Ab profiles. The sera from these patients either with AoDILD or in the stable state were combined; the two pooled sera at these two states were screened for the auto-Ab profiling to be described in the following sections (Fig. 1).

Bottom Line: It is then designated as collagen vascular disease-associated ILD (CVD-ILD), and influences patients' prognosis.The ratio of the average anti-MICA Ab level to the average anti-human leukocyte antigen class I Ab level (ie, MICA/Class I) was significantly higher in RA patients with CVD-ILD compared with those without (P = 4.47 × 10(-5)).The MICA/Class I ratio could be a better marker for diagnosing CVD-ILD than KL-6 (Krebs von den lungen-6).

View Article: PubMed Central - PubMed

Affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara Hospital, National Hospital Organization, Sagamihara, Japan. ; Molecular and Genetic Epidemiology Laboratory, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.

ABSTRACT
Interstitial lung disease (ILD) is frequently associated with collagen disease. It is then designated as collagen vascular disease-associated ILD (CVD-ILD), and influences patients' prognosis. The prognosis of acute-onset diffuse ILD (AoDILD) occurring in patients with collagen disease is quite poor. Here, we report our investigation of auto-antibody (Ab) profiles to determine whether they may be useful in diagnosing CVD-ILD or AoDILD in collagen disease. Auto-Ab profiles were analyzed using the Lambda Array Beads Multi-Analyte System, granulocyte immunofluorescence test, Proto-Array Human Protein Microarray, AlphaScreen assay, and glutathione S-transferase capture enzyme-linked immunosorbent assay in 34 patients with rheumatoid arthritis (RA) with or without CVD-ILD and in 15 patients with collagen disease with AoDILD. The average anti-major histocompatibility complex class I-related chain A (MICA) Ab levels were higher in RA patients with CVD-ILD than in those without (P = 0.0013). The ratio of the average anti-MICA Ab level to the average anti-human leukocyte antigen class I Ab level (ie, MICA/Class I) was significantly higher in RA patients with CVD-ILD compared with those without (P = 4.47 × 10(-5)). To the best of our knowledge, this is the first report of auto-Ab profiles in CVD-ILD. The MICA/Class I ratio could be a better marker for diagnosing CVD-ILD than KL-6 (Krebs von den lungen-6).

No MeSH data available.


Related in: MedlinePlus