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The histone demethylase PHF8 promotes prostate cancer cell growth by activating the oncomiR miR-125b.

Ma Q, Chen Z, Jia G, Lu X, Xie X, Jin W - Onco Targets Ther (2015)

Bottom Line: Furthermore, miR-125b is a target of PHF8, and miR-125b seems to be essential for the hyper proliferation of PCa cells in the presence of PHF8.In conclusion, we identify the histone demethylase PHF8 as an oncogenic protein in human PCa.These findings indicate PHF8 as a potential candidate for clinical intervention.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Jinshan Hospital, Fudan University, Shanghai, People's Republic of China.

ABSTRACT

Aims: Prostate cancer (PCa) is the most frequently diagnosed malignancy in men. However, the underlying mechanism is not fully understood. In this study, we aim to research the molecular mechanisms underlying the initiation and progression of PCa.

Results: Plant homeodomain finger protein 8 (PHF8) is upregulated in human PCa tissues and cell lines. PHF8 knockdown attenuates growth and cellular transformation of PCa cells. PHF8 depletion induces PCa cell apoptosis by activating proapoptotic proteins and inactivating antiapoptotic proteins. Furthermore, miR-125b is a target of PHF8, and miR-125b seems to be essential for the hyper proliferation of PCa cells in the presence of PHF8.

Conclusion: In conclusion, we identify the histone demethylase PHF8 as an oncogenic protein in human PCa. These findings indicate PHF8 as a potential candidate for clinical intervention.

No MeSH data available.


Related in: MedlinePlus

PHF8 is upregulated in human PCa and cell lines.Notes: (A) PHF8 mRNA level is upregulated in human PCa. The mRNA level of PHF8 in adjacent normal tissues and cancer tissues from PCa patients was analyzed with q-PCR. N=19 in normal group; n=89 in PCa group. (B) Representative IHC results showing PHF8 protein level is upregulated in human PCa. Adjacent normal prostate tissues and PCa tissues were subjected to IHC analyses with anti-PHF8 antibody. Both low PHF8 expression and high PHF8 expression are shown. Scale is 50 μm. (C) PHF8 mRNA level is high in PCa cell lines. The mRNA level of PHF8 in normal prostate epithelial cells (PNT2 and BPH-1) and PCa cells (LNCaP, PC-3, and DU145) was analyzed with q-PCR.Abbreviations: PHF8, plant homeodomain finger protein 8; PCa, prostate cancer; IHC, immunohistochemical.
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f1-ott-8-1979: PHF8 is upregulated in human PCa and cell lines.Notes: (A) PHF8 mRNA level is upregulated in human PCa. The mRNA level of PHF8 in adjacent normal tissues and cancer tissues from PCa patients was analyzed with q-PCR. N=19 in normal group; n=89 in PCa group. (B) Representative IHC results showing PHF8 protein level is upregulated in human PCa. Adjacent normal prostate tissues and PCa tissues were subjected to IHC analyses with anti-PHF8 antibody. Both low PHF8 expression and high PHF8 expression are shown. Scale is 50 μm. (C) PHF8 mRNA level is high in PCa cell lines. The mRNA level of PHF8 in normal prostate epithelial cells (PNT2 and BPH-1) and PCa cells (LNCaP, PC-3, and DU145) was analyzed with q-PCR.Abbreviations: PHF8, plant homeodomain finger protein 8; PCa, prostate cancer; IHC, immunohistochemical.

Mentions: To investigate the potential role of the histone demethylase PHF8 in human PCa, we first profiled the expression pattern of PHF8 in human normal prostate tissues and cancer tissues. We analyzed 19 normal tissues and 89 PCa tissues and found that PHF8 was upregulated in human PCa tissues at both mRNA and protein levels (Figure 1A and B). Furthermore, we analyzed the expression of PHF8 in three human PCa cell lines (LNCaP, PC-3, and DU145) and normal prostate epithelial cell lines (PNT2 and BPH-1). The results showed that PHF8 mRNA levels were high in PCa cells compared to normal epithelial cells (Figure 1C). Taken together, these findings demonstrate that PHF8 is upregulated in human PCa and indicate the potential role of PHF8 in human PCa.


The histone demethylase PHF8 promotes prostate cancer cell growth by activating the oncomiR miR-125b.

Ma Q, Chen Z, Jia G, Lu X, Xie X, Jin W - Onco Targets Ther (2015)

PHF8 is upregulated in human PCa and cell lines.Notes: (A) PHF8 mRNA level is upregulated in human PCa. The mRNA level of PHF8 in adjacent normal tissues and cancer tissues from PCa patients was analyzed with q-PCR. N=19 in normal group; n=89 in PCa group. (B) Representative IHC results showing PHF8 protein level is upregulated in human PCa. Adjacent normal prostate tissues and PCa tissues were subjected to IHC analyses with anti-PHF8 antibody. Both low PHF8 expression and high PHF8 expression are shown. Scale is 50 μm. (C) PHF8 mRNA level is high in PCa cell lines. The mRNA level of PHF8 in normal prostate epithelial cells (PNT2 and BPH-1) and PCa cells (LNCaP, PC-3, and DU145) was analyzed with q-PCR.Abbreviations: PHF8, plant homeodomain finger protein 8; PCa, prostate cancer; IHC, immunohistochemical.
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Related In: Results  -  Collection

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f1-ott-8-1979: PHF8 is upregulated in human PCa and cell lines.Notes: (A) PHF8 mRNA level is upregulated in human PCa. The mRNA level of PHF8 in adjacent normal tissues and cancer tissues from PCa patients was analyzed with q-PCR. N=19 in normal group; n=89 in PCa group. (B) Representative IHC results showing PHF8 protein level is upregulated in human PCa. Adjacent normal prostate tissues and PCa tissues were subjected to IHC analyses with anti-PHF8 antibody. Both low PHF8 expression and high PHF8 expression are shown. Scale is 50 μm. (C) PHF8 mRNA level is high in PCa cell lines. The mRNA level of PHF8 in normal prostate epithelial cells (PNT2 and BPH-1) and PCa cells (LNCaP, PC-3, and DU145) was analyzed with q-PCR.Abbreviations: PHF8, plant homeodomain finger protein 8; PCa, prostate cancer; IHC, immunohistochemical.
Mentions: To investigate the potential role of the histone demethylase PHF8 in human PCa, we first profiled the expression pattern of PHF8 in human normal prostate tissues and cancer tissues. We analyzed 19 normal tissues and 89 PCa tissues and found that PHF8 was upregulated in human PCa tissues at both mRNA and protein levels (Figure 1A and B). Furthermore, we analyzed the expression of PHF8 in three human PCa cell lines (LNCaP, PC-3, and DU145) and normal prostate epithelial cell lines (PNT2 and BPH-1). The results showed that PHF8 mRNA levels were high in PCa cells compared to normal epithelial cells (Figure 1C). Taken together, these findings demonstrate that PHF8 is upregulated in human PCa and indicate the potential role of PHF8 in human PCa.

Bottom Line: Furthermore, miR-125b is a target of PHF8, and miR-125b seems to be essential for the hyper proliferation of PCa cells in the presence of PHF8.In conclusion, we identify the histone demethylase PHF8 as an oncogenic protein in human PCa.These findings indicate PHF8 as a potential candidate for clinical intervention.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Jinshan Hospital, Fudan University, Shanghai, People's Republic of China.

ABSTRACT

Aims: Prostate cancer (PCa) is the most frequently diagnosed malignancy in men. However, the underlying mechanism is not fully understood. In this study, we aim to research the molecular mechanisms underlying the initiation and progression of PCa.

Results: Plant homeodomain finger protein 8 (PHF8) is upregulated in human PCa tissues and cell lines. PHF8 knockdown attenuates growth and cellular transformation of PCa cells. PHF8 depletion induces PCa cell apoptosis by activating proapoptotic proteins and inactivating antiapoptotic proteins. Furthermore, miR-125b is a target of PHF8, and miR-125b seems to be essential for the hyper proliferation of PCa cells in the presence of PHF8.

Conclusion: In conclusion, we identify the histone demethylase PHF8 as an oncogenic protein in human PCa. These findings indicate PHF8 as a potential candidate for clinical intervention.

No MeSH data available.


Related in: MedlinePlus