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Rat Nasal Respiratory Mucosa-Derived Ectomesenchymal Stem Cells Differentiate into Schwann-Like Cells Promoting the Differentiation of PC12 Cells and Forming Myelin In Vitro.

Zhang J, Gao X, Zou H, Liu J, Zhang Z - Stem Cells Int (2015)

Bottom Line: When cocultured with dREMSCs for 5 days, PC12 cells differentiated into mature neuron-like cells with long neurites.More importantly, dREMSCs could form myelin structures with the neurites of PC12 cells at 21 days in vitro.Our data indicated that REMSCs, a kind of EMSCs, could differentiate into SC-like cells and have the ability to promote the differentiation of PC12 cells and form myelin in vitro.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou 213003, China.

ABSTRACT
Schwann cell (SC) transplantation as a cell-based therapy can enhance peripheral and central nerve repair experimentally, but it is limited by the donor site morbidity for clinical application. We investigated weather respiratory mucosa stem cells (REMSCs), a kind of ectomesenchymal stem cells (EMSCs), isolated from rat nasal septum can differentiate into functional Schwann-like cells (SC-like cells). REMSCs proliferated quickly in vitro and expressed the neural crest markers (nestin, vimentin, SOX10, and CD44). Treated with a mixture of glial growth factors for 7 days, REMSCs differentiated into SC-like cells. The differentiated REMSCs (dREMSCs) exhibited a spindle-like morphology similar to SC cells. Immunocytochemical staining and Western blotting indicated that SC-like cells expressed the glial markers (GFAP, S100β, Galc, and P75) and CNPase. When cocultured with dREMSCs for 5 days, PC12 cells differentiated into mature neuron-like cells with long neurites. More importantly, dREMSCs could form myelin structures with the neurites of PC12 cells at 21 days in vitro. Our data indicated that REMSCs, a kind of EMSCs, could differentiate into SC-like cells and have the ability to promote the differentiation of PC12 cells and form myelin in vitro.

No MeSH data available.


Related in: MedlinePlus

Western blotting showed the upregulation of expression of Schwann cell markers and the downregulation of expression of neural crest markers except for SOX10. Expressions of Schwann cell markers including GFAP, CNPase, P75, and S100β by REMSCs and SC-like cells were shown in (a); expressions of neural crest markers including SOX10, nestin, vimentin, and CD44 by REMSCs and SC-like cells were shown in (b); the experiments were replicated three times, and β-actin was used as a loading control. Quantitation of each marker was calculated using morphometric analysis with ImageJ software. Each bar showed the ratio of the expression level of marker protein to β-actin (c, d). The data were presented as mean ± SEM of three independent experiments. **P < 0.01 represented significant differences when compared between RMSCs and SC-like cells.
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fig4: Western blotting showed the upregulation of expression of Schwann cell markers and the downregulation of expression of neural crest markers except for SOX10. Expressions of Schwann cell markers including GFAP, CNPase, P75, and S100β by REMSCs and SC-like cells were shown in (a); expressions of neural crest markers including SOX10, nestin, vimentin, and CD44 by REMSCs and SC-like cells were shown in (b); the experiments were replicated three times, and β-actin was used as a loading control. Quantitation of each marker was calculated using morphometric analysis with ImageJ software. Each bar showed the ratio of the expression level of marker protein to β-actin (c, d). The data were presented as mean ± SEM of three independent experiments. **P < 0.01 represented significant differences when compared between RMSCs and SC-like cells.

Mentions: To further confirm the immunofluorescent staining results, Western blot analysis was used to examine the expression of glial specific markers and neural crest markers (Figure 4). β-Actin was used as a loading reference. The expression level of each protein was expressed as the ratio of the expression level of the marker protein to β-actin. The expression levels of GFAP, CNPase, P75, and S100β in SC-like cells were more pronounced compared to REMSCs (P < 0.01) (Figures 4(a) and 4(c)). In addition, Figures 4(b) and 4(d) showed the downregulation of nestin, vimentin, and CD44 proteins in SC-like cells (P < 0.01). However, the level of SOX10 was similar in REMSCs and SC-like cells (P > 0.05) (Figures 4(b) and 4(d)).


Rat Nasal Respiratory Mucosa-Derived Ectomesenchymal Stem Cells Differentiate into Schwann-Like Cells Promoting the Differentiation of PC12 Cells and Forming Myelin In Vitro.

Zhang J, Gao X, Zou H, Liu J, Zhang Z - Stem Cells Int (2015)

Western blotting showed the upregulation of expression of Schwann cell markers and the downregulation of expression of neural crest markers except for SOX10. Expressions of Schwann cell markers including GFAP, CNPase, P75, and S100β by REMSCs and SC-like cells were shown in (a); expressions of neural crest markers including SOX10, nestin, vimentin, and CD44 by REMSCs and SC-like cells were shown in (b); the experiments were replicated three times, and β-actin was used as a loading control. Quantitation of each marker was calculated using morphometric analysis with ImageJ software. Each bar showed the ratio of the expression level of marker protein to β-actin (c, d). The data were presented as mean ± SEM of three independent experiments. **P < 0.01 represented significant differences when compared between RMSCs and SC-like cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig4: Western blotting showed the upregulation of expression of Schwann cell markers and the downregulation of expression of neural crest markers except for SOX10. Expressions of Schwann cell markers including GFAP, CNPase, P75, and S100β by REMSCs and SC-like cells were shown in (a); expressions of neural crest markers including SOX10, nestin, vimentin, and CD44 by REMSCs and SC-like cells were shown in (b); the experiments were replicated three times, and β-actin was used as a loading control. Quantitation of each marker was calculated using morphometric analysis with ImageJ software. Each bar showed the ratio of the expression level of marker protein to β-actin (c, d). The data were presented as mean ± SEM of three independent experiments. **P < 0.01 represented significant differences when compared between RMSCs and SC-like cells.
Mentions: To further confirm the immunofluorescent staining results, Western blot analysis was used to examine the expression of glial specific markers and neural crest markers (Figure 4). β-Actin was used as a loading reference. The expression level of each protein was expressed as the ratio of the expression level of the marker protein to β-actin. The expression levels of GFAP, CNPase, P75, and S100β in SC-like cells were more pronounced compared to REMSCs (P < 0.01) (Figures 4(a) and 4(c)). In addition, Figures 4(b) and 4(d) showed the downregulation of nestin, vimentin, and CD44 proteins in SC-like cells (P < 0.01). However, the level of SOX10 was similar in REMSCs and SC-like cells (P > 0.05) (Figures 4(b) and 4(d)).

Bottom Line: When cocultured with dREMSCs for 5 days, PC12 cells differentiated into mature neuron-like cells with long neurites.More importantly, dREMSCs could form myelin structures with the neurites of PC12 cells at 21 days in vitro.Our data indicated that REMSCs, a kind of EMSCs, could differentiate into SC-like cells and have the ability to promote the differentiation of PC12 cells and form myelin in vitro.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou 213003, China.

ABSTRACT
Schwann cell (SC) transplantation as a cell-based therapy can enhance peripheral and central nerve repair experimentally, but it is limited by the donor site morbidity for clinical application. We investigated weather respiratory mucosa stem cells (REMSCs), a kind of ectomesenchymal stem cells (EMSCs), isolated from rat nasal septum can differentiate into functional Schwann-like cells (SC-like cells). REMSCs proliferated quickly in vitro and expressed the neural crest markers (nestin, vimentin, SOX10, and CD44). Treated with a mixture of glial growth factors for 7 days, REMSCs differentiated into SC-like cells. The differentiated REMSCs (dREMSCs) exhibited a spindle-like morphology similar to SC cells. Immunocytochemical staining and Western blotting indicated that SC-like cells expressed the glial markers (GFAP, S100β, Galc, and P75) and CNPase. When cocultured with dREMSCs for 5 days, PC12 cells differentiated into mature neuron-like cells with long neurites. More importantly, dREMSCs could form myelin structures with the neurites of PC12 cells at 21 days in vitro. Our data indicated that REMSCs, a kind of EMSCs, could differentiate into SC-like cells and have the ability to promote the differentiation of PC12 cells and form myelin in vitro.

No MeSH data available.


Related in: MedlinePlus