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Rat Nasal Respiratory Mucosa-Derived Ectomesenchymal Stem Cells Differentiate into Schwann-Like Cells Promoting the Differentiation of PC12 Cells and Forming Myelin In Vitro.

Zhang J, Gao X, Zou H, Liu J, Zhang Z - Stem Cells Int (2015)

Bottom Line: When cocultured with dREMSCs for 5 days, PC12 cells differentiated into mature neuron-like cells with long neurites.More importantly, dREMSCs could form myelin structures with the neurites of PC12 cells at 21 days in vitro.Our data indicated that REMSCs, a kind of EMSCs, could differentiate into SC-like cells and have the ability to promote the differentiation of PC12 cells and form myelin in vitro.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou 213003, China.

ABSTRACT
Schwann cell (SC) transplantation as a cell-based therapy can enhance peripheral and central nerve repair experimentally, but it is limited by the donor site morbidity for clinical application. We investigated weather respiratory mucosa stem cells (REMSCs), a kind of ectomesenchymal stem cells (EMSCs), isolated from rat nasal septum can differentiate into functional Schwann-like cells (SC-like cells). REMSCs proliferated quickly in vitro and expressed the neural crest markers (nestin, vimentin, SOX10, and CD44). Treated with a mixture of glial growth factors for 7 days, REMSCs differentiated into SC-like cells. The differentiated REMSCs (dREMSCs) exhibited a spindle-like morphology similar to SC cells. Immunocytochemical staining and Western blotting indicated that SC-like cells expressed the glial markers (GFAP, S100β, Galc, and P75) and CNPase. When cocultured with dREMSCs for 5 days, PC12 cells differentiated into mature neuron-like cells with long neurites. More importantly, dREMSCs could form myelin structures with the neurites of PC12 cells at 21 days in vitro. Our data indicated that REMSCs, a kind of EMSCs, could differentiate into SC-like cells and have the ability to promote the differentiation of PC12 cells and form myelin in vitro.

No MeSH data available.


Related in: MedlinePlus

SC-like cells differentiated from REMSCs demonstrated typical Schwann cell morphology and were labeled with related cell surface markers. After 10 days, cells appeared typical bipolar and spindle-like Schwann cell phenotype (a), immunofluorescent staining showed that differentiated cells were stained positively with GFAP (b), P75 (c), S100β (d), Galc (e), and CNPase (f). Nuclei were labeled with Hoechst 33342 (blue). Bar: 50 μm for all pictures.
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fig3: SC-like cells differentiated from REMSCs demonstrated typical Schwann cell morphology and were labeled with related cell surface markers. After 10 days, cells appeared typical bipolar and spindle-like Schwann cell phenotype (a), immunofluorescent staining showed that differentiated cells were stained positively with GFAP (b), P75 (c), S100β (d), Galc (e), and CNPase (f). Nuclei were labeled with Hoechst 33342 (blue). Bar: 50 μm for all pictures.

Mentions: REMSCs were treated with SCDM containing a mixture of glial cell growth factors for 10 days. The morphology of SC-like cells and the expression of the SC proteins such as GFAP, S100β, Galc, CNPase, and P75 were examined. After inductive differentiation, rat REMSCs changed from fibroblast-like morphology to spindle shape that seems to be more elongated than before, and these cells could continue to proliferate (Figure 3(a)). Immunofluorescent staining showed that about 79 ± 1.2% of the differentiated cells were positive for GFAP (Figure 3(b)); 81.7 ± 1.0% of the differentiated REMSCs were positive for P75 (Figure 3(c)); 89.2 ± 1.6% of the differentiated REMSCs were positive for S100β (Figure 3(d)); 84.9 ± 0.9% of the differentiated REMSCs were positive for Galc (Figure 3(e)), 84.6 ± 1.9% of the differentiated REMSCs were positive for CNPase (Figure 3(f)).


Rat Nasal Respiratory Mucosa-Derived Ectomesenchymal Stem Cells Differentiate into Schwann-Like Cells Promoting the Differentiation of PC12 Cells and Forming Myelin In Vitro.

Zhang J, Gao X, Zou H, Liu J, Zhang Z - Stem Cells Int (2015)

SC-like cells differentiated from REMSCs demonstrated typical Schwann cell morphology and were labeled with related cell surface markers. After 10 days, cells appeared typical bipolar and spindle-like Schwann cell phenotype (a), immunofluorescent staining showed that differentiated cells were stained positively with GFAP (b), P75 (c), S100β (d), Galc (e), and CNPase (f). Nuclei were labeled with Hoechst 33342 (blue). Bar: 50 μm for all pictures.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4539076&req=5

fig3: SC-like cells differentiated from REMSCs demonstrated typical Schwann cell morphology and were labeled with related cell surface markers. After 10 days, cells appeared typical bipolar and spindle-like Schwann cell phenotype (a), immunofluorescent staining showed that differentiated cells were stained positively with GFAP (b), P75 (c), S100β (d), Galc (e), and CNPase (f). Nuclei were labeled with Hoechst 33342 (blue). Bar: 50 μm for all pictures.
Mentions: REMSCs were treated with SCDM containing a mixture of glial cell growth factors for 10 days. The morphology of SC-like cells and the expression of the SC proteins such as GFAP, S100β, Galc, CNPase, and P75 were examined. After inductive differentiation, rat REMSCs changed from fibroblast-like morphology to spindle shape that seems to be more elongated than before, and these cells could continue to proliferate (Figure 3(a)). Immunofluorescent staining showed that about 79 ± 1.2% of the differentiated cells were positive for GFAP (Figure 3(b)); 81.7 ± 1.0% of the differentiated REMSCs were positive for P75 (Figure 3(c)); 89.2 ± 1.6% of the differentiated REMSCs were positive for S100β (Figure 3(d)); 84.9 ± 0.9% of the differentiated REMSCs were positive for Galc (Figure 3(e)), 84.6 ± 1.9% of the differentiated REMSCs were positive for CNPase (Figure 3(f)).

Bottom Line: When cocultured with dREMSCs for 5 days, PC12 cells differentiated into mature neuron-like cells with long neurites.More importantly, dREMSCs could form myelin structures with the neurites of PC12 cells at 21 days in vitro.Our data indicated that REMSCs, a kind of EMSCs, could differentiate into SC-like cells and have the ability to promote the differentiation of PC12 cells and form myelin in vitro.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou 213003, China.

ABSTRACT
Schwann cell (SC) transplantation as a cell-based therapy can enhance peripheral and central nerve repair experimentally, but it is limited by the donor site morbidity for clinical application. We investigated weather respiratory mucosa stem cells (REMSCs), a kind of ectomesenchymal stem cells (EMSCs), isolated from rat nasal septum can differentiate into functional Schwann-like cells (SC-like cells). REMSCs proliferated quickly in vitro and expressed the neural crest markers (nestin, vimentin, SOX10, and CD44). Treated with a mixture of glial growth factors for 7 days, REMSCs differentiated into SC-like cells. The differentiated REMSCs (dREMSCs) exhibited a spindle-like morphology similar to SC cells. Immunocytochemical staining and Western blotting indicated that SC-like cells expressed the glial markers (GFAP, S100β, Galc, and P75) and CNPase. When cocultured with dREMSCs for 5 days, PC12 cells differentiated into mature neuron-like cells with long neurites. More importantly, dREMSCs could form myelin structures with the neurites of PC12 cells at 21 days in vitro. Our data indicated that REMSCs, a kind of EMSCs, could differentiate into SC-like cells and have the ability to promote the differentiation of PC12 cells and form myelin in vitro.

No MeSH data available.


Related in: MedlinePlus