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Rat Nasal Respiratory Mucosa-Derived Ectomesenchymal Stem Cells Differentiate into Schwann-Like Cells Promoting the Differentiation of PC12 Cells and Forming Myelin In Vitro.

Zhang J, Gao X, Zou H, Liu J, Zhang Z - Stem Cells Int (2015)

Bottom Line: When cocultured with dREMSCs for 5 days, PC12 cells differentiated into mature neuron-like cells with long neurites.More importantly, dREMSCs could form myelin structures with the neurites of PC12 cells at 21 days in vitro.Our data indicated that REMSCs, a kind of EMSCs, could differentiate into SC-like cells and have the ability to promote the differentiation of PC12 cells and form myelin in vitro.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou 213003, China.

ABSTRACT
Schwann cell (SC) transplantation as a cell-based therapy can enhance peripheral and central nerve repair experimentally, but it is limited by the donor site morbidity for clinical application. We investigated weather respiratory mucosa stem cells (REMSCs), a kind of ectomesenchymal stem cells (EMSCs), isolated from rat nasal septum can differentiate into functional Schwann-like cells (SC-like cells). REMSCs proliferated quickly in vitro and expressed the neural crest markers (nestin, vimentin, SOX10, and CD44). Treated with a mixture of glial growth factors for 7 days, REMSCs differentiated into SC-like cells. The differentiated REMSCs (dREMSCs) exhibited a spindle-like morphology similar to SC cells. Immunocytochemical staining and Western blotting indicated that SC-like cells expressed the glial markers (GFAP, S100β, Galc, and P75) and CNPase. When cocultured with dREMSCs for 5 days, PC12 cells differentiated into mature neuron-like cells with long neurites. More importantly, dREMSCs could form myelin structures with the neurites of PC12 cells at 21 days in vitro. Our data indicated that REMSCs, a kind of EMSCs, could differentiate into SC-like cells and have the ability to promote the differentiation of PC12 cells and form myelin in vitro.

No MeSH data available.


Related in: MedlinePlus

Labeling of neural crest cell markers on REMSCs: those markers were positively stained for SOX10 (a), nestin (b), vimentin (c), and CD44 (d). Nuclei were labeled with Hoechst 33342 (blue) except for SOX10. Bar: 20 μm for all pictures.
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fig2: Labeling of neural crest cell markers on REMSCs: those markers were positively stained for SOX10 (a), nestin (b), vimentin (c), and CD44 (d). Nuclei were labeled with Hoechst 33342 (blue) except for SOX10. Bar: 20 μm for all pictures.

Mentions: After 5 days, adherent cells migrated from the explants and formed colonies (Figure 1(a)). REMSCs at 4th passage appeared as fibroblastic-like cells and proliferated rapidly on plastic plates (Figure 1(b)). Immunofluorescent staining showed almost all of the REMSCs expressed neural crest cell markers such as SOX10 (87.6 ± 0.7%), nestin (90.8 ± 0.8%), vimentin (92.2 ± 0.8%), and CD44 (88.1 ± 0.8%) (Figures 2(a)–2(d)).


Rat Nasal Respiratory Mucosa-Derived Ectomesenchymal Stem Cells Differentiate into Schwann-Like Cells Promoting the Differentiation of PC12 Cells and Forming Myelin In Vitro.

Zhang J, Gao X, Zou H, Liu J, Zhang Z - Stem Cells Int (2015)

Labeling of neural crest cell markers on REMSCs: those markers were positively stained for SOX10 (a), nestin (b), vimentin (c), and CD44 (d). Nuclei were labeled with Hoechst 33342 (blue) except for SOX10. Bar: 20 μm for all pictures.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4539076&req=5

fig2: Labeling of neural crest cell markers on REMSCs: those markers were positively stained for SOX10 (a), nestin (b), vimentin (c), and CD44 (d). Nuclei were labeled with Hoechst 33342 (blue) except for SOX10. Bar: 20 μm for all pictures.
Mentions: After 5 days, adherent cells migrated from the explants and formed colonies (Figure 1(a)). REMSCs at 4th passage appeared as fibroblastic-like cells and proliferated rapidly on plastic plates (Figure 1(b)). Immunofluorescent staining showed almost all of the REMSCs expressed neural crest cell markers such as SOX10 (87.6 ± 0.7%), nestin (90.8 ± 0.8%), vimentin (92.2 ± 0.8%), and CD44 (88.1 ± 0.8%) (Figures 2(a)–2(d)).

Bottom Line: When cocultured with dREMSCs for 5 days, PC12 cells differentiated into mature neuron-like cells with long neurites.More importantly, dREMSCs could form myelin structures with the neurites of PC12 cells at 21 days in vitro.Our data indicated that REMSCs, a kind of EMSCs, could differentiate into SC-like cells and have the ability to promote the differentiation of PC12 cells and form myelin in vitro.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou 213003, China.

ABSTRACT
Schwann cell (SC) transplantation as a cell-based therapy can enhance peripheral and central nerve repair experimentally, but it is limited by the donor site morbidity for clinical application. We investigated weather respiratory mucosa stem cells (REMSCs), a kind of ectomesenchymal stem cells (EMSCs), isolated from rat nasal septum can differentiate into functional Schwann-like cells (SC-like cells). REMSCs proliferated quickly in vitro and expressed the neural crest markers (nestin, vimentin, SOX10, and CD44). Treated with a mixture of glial growth factors for 7 days, REMSCs differentiated into SC-like cells. The differentiated REMSCs (dREMSCs) exhibited a spindle-like morphology similar to SC cells. Immunocytochemical staining and Western blotting indicated that SC-like cells expressed the glial markers (GFAP, S100β, Galc, and P75) and CNPase. When cocultured with dREMSCs for 5 days, PC12 cells differentiated into mature neuron-like cells with long neurites. More importantly, dREMSCs could form myelin structures with the neurites of PC12 cells at 21 days in vitro. Our data indicated that REMSCs, a kind of EMSCs, could differentiate into SC-like cells and have the ability to promote the differentiation of PC12 cells and form myelin in vitro.

No MeSH data available.


Related in: MedlinePlus