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Gigantol Suppresses Cancer Stem Cell-Like Phenotypes in Lung Cancer Cells.

Bhummaphan N, Chanvorachote P - Evid Based Complement Alternat Med (2015)

Bottom Line: Importantly, gigantol significantly reduced the ability of the cancer cells to form tumor spheroids, a critical hallmark of CSCs.Concomitantly, the treatment of the compound was shown to reduce well-known lung CSCs markers, including CD133 and ALDH1A1.In conclusion, gigantol possesses CSCs suppressing activity which may facilitate the development of this compound for therapeutic approaches by targeting CSCs.

View Article: PubMed Central - PubMed

Affiliation: Cell-Based Drug and Health Product Development Research Unit, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.

ABSTRACT
As cancer stem cells (CSCs) contribute to malignancy, metastasis, and relapse of cancers, potential of compound in inhibition of CSCs has garnered most attention in the cancer research as well as drug development fields recently. Herein, we have demonstrated for the first time that gigantol, a pure compound isolated from Dendrobium draconis, dramatically suppressed stem-like phenotypes of human lung cancer cells. Gigantol at nontoxic concentrations significantly reduced anchorage-independent growth and survival of the cancer cells. Importantly, gigantol significantly reduced the ability of the cancer cells to form tumor spheroids, a critical hallmark of CSCs. Concomitantly, the treatment of the compound was shown to reduce well-known lung CSCs markers, including CD133 and ALDH1A1. Moreover, we revealed that gigantol decreased stemness in the cancer cells by suppressing the activation of protein kinase B (Akt) signal which in turn decreased the cellular levels of pluripotency and self-renewal factors Oct4 and Nanog. In conclusion, gigantol possesses CSCs suppressing activity which may facilitate the development of this compound for therapeutic approaches by targeting CSCs.

No MeSH data available.


Related in: MedlinePlus

Gigantol inhibits anchorage-independent growth of human lung cancer H460 cells. (a) After being treated with gigantol (0–20 µM) for 48 h, H460 cells were suspended and subjected to anchorage-independent growth assay. (b) Colony number and size were analyzed and calculated as relative values to the control cells. Colony 4x images were captured after day 10. All plots are means ± SD (n = 3). ∗P < 0.05 versus nontreated cells.
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fig2: Gigantol inhibits anchorage-independent growth of human lung cancer H460 cells. (a) After being treated with gigantol (0–20 µM) for 48 h, H460 cells were suspended and subjected to anchorage-independent growth assay. (b) Colony number and size were analyzed and calculated as relative values to the control cells. Colony 4x images were captured after day 10. All plots are means ± SD (n = 3). ∗P < 0.05 versus nontreated cells.

Mentions: As the ability of the cancer cells to form spheroids as well as growth and survival in anchorage-independent condition has been widely accepted as a hallmark of CSCs, we next tested the effect of gigantol on such behaviors. H460 cells were treated with noncytotoxic concentrations of gigantol (0–20 µM) for 48 h, and the cells were subjected to anchorage-independent growth and spheroid formation assays. For anchorage-independent growth, the colony number and colony size were determined and presented as relative values in comparison to those of nontreated control. Figure 2(a) shows that treatment of the cells with gigantol resulted in the significant decrease of colony number and colony size in a dose-dependent manner. A significant suppression was first detected at 5 µM of gigantol with approximately 30% reduction in terms of colony number and 20% reduction in terms of size.


Gigantol Suppresses Cancer Stem Cell-Like Phenotypes in Lung Cancer Cells.

Bhummaphan N, Chanvorachote P - Evid Based Complement Alternat Med (2015)

Gigantol inhibits anchorage-independent growth of human lung cancer H460 cells. (a) After being treated with gigantol (0–20 µM) for 48 h, H460 cells were suspended and subjected to anchorage-independent growth assay. (b) Colony number and size were analyzed and calculated as relative values to the control cells. Colony 4x images were captured after day 10. All plots are means ± SD (n = 3). ∗P < 0.05 versus nontreated cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4539074&req=5

fig2: Gigantol inhibits anchorage-independent growth of human lung cancer H460 cells. (a) After being treated with gigantol (0–20 µM) for 48 h, H460 cells were suspended and subjected to anchorage-independent growth assay. (b) Colony number and size were analyzed and calculated as relative values to the control cells. Colony 4x images were captured after day 10. All plots are means ± SD (n = 3). ∗P < 0.05 versus nontreated cells.
Mentions: As the ability of the cancer cells to form spheroids as well as growth and survival in anchorage-independent condition has been widely accepted as a hallmark of CSCs, we next tested the effect of gigantol on such behaviors. H460 cells were treated with noncytotoxic concentrations of gigantol (0–20 µM) for 48 h, and the cells were subjected to anchorage-independent growth and spheroid formation assays. For anchorage-independent growth, the colony number and colony size were determined and presented as relative values in comparison to those of nontreated control. Figure 2(a) shows that treatment of the cells with gigantol resulted in the significant decrease of colony number and colony size in a dose-dependent manner. A significant suppression was first detected at 5 µM of gigantol with approximately 30% reduction in terms of colony number and 20% reduction in terms of size.

Bottom Line: Importantly, gigantol significantly reduced the ability of the cancer cells to form tumor spheroids, a critical hallmark of CSCs.Concomitantly, the treatment of the compound was shown to reduce well-known lung CSCs markers, including CD133 and ALDH1A1.In conclusion, gigantol possesses CSCs suppressing activity which may facilitate the development of this compound for therapeutic approaches by targeting CSCs.

View Article: PubMed Central - PubMed

Affiliation: Cell-Based Drug and Health Product Development Research Unit, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.

ABSTRACT
As cancer stem cells (CSCs) contribute to malignancy, metastasis, and relapse of cancers, potential of compound in inhibition of CSCs has garnered most attention in the cancer research as well as drug development fields recently. Herein, we have demonstrated for the first time that gigantol, a pure compound isolated from Dendrobium draconis, dramatically suppressed stem-like phenotypes of human lung cancer cells. Gigantol at nontoxic concentrations significantly reduced anchorage-independent growth and survival of the cancer cells. Importantly, gigantol significantly reduced the ability of the cancer cells to form tumor spheroids, a critical hallmark of CSCs. Concomitantly, the treatment of the compound was shown to reduce well-known lung CSCs markers, including CD133 and ALDH1A1. Moreover, we revealed that gigantol decreased stemness in the cancer cells by suppressing the activation of protein kinase B (Akt) signal which in turn decreased the cellular levels of pluripotency and self-renewal factors Oct4 and Nanog. In conclusion, gigantol possesses CSCs suppressing activity which may facilitate the development of this compound for therapeutic approaches by targeting CSCs.

No MeSH data available.


Related in: MedlinePlus