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The Immunomodulatory Effects of Nidus Vespae on Human Peripheral Blood Immune Cells In Vitro.

Zhu M, Ling Y, Qi Q, Zhang Y, Bao Y, Liu Y - Evid Based Complement Alternat Med (2015)

Bottom Line: Data showed that NVD has no significant inhibiting effects on gastric cancer cells growth.Nevertheless, it could obviously promote PBMC proliferation in a time- and concentration-dependent manner.After treatment with NVD, the CTL cytotoxicity against SGC-7901 was significantly greater than control.

View Article: PubMed Central - PubMed

Affiliation: Clinical Oncology Laboratory, Changzhou Tumor Hospital Affiliated to Soochow University, Huaide Road 44, Changzhou 213002, China.

ABSTRACT
Nidus Vespae has been used in traditional Chinese medicine (TCM) to treat various cancers, but the underlying mechanisms were not yet clarified. This study was to investigate the effect of Nidus Vespae decoction (NVD) on tumor cell viability and immunoregulating functions of human peripheral blood immune cells. The effects on tumor cell viability, peripheral blood mononuclear cell (PBMC) proliferation activity, and the tumor cell phagocytosis of monocytes were evaluated by cell counting kit-8. Tumor-killing activity of cytotoxic T lymphocyte (CTL) was analyzed by (51)Cr releasing assay. IgG production of B cells and cytokine (TNF-α and IL-6) secretion of monocytes were determined by ELISA method. Data showed that NVD has no significant inhibiting effects on gastric cancer cells growth. Nevertheless, it could obviously promote PBMC proliferation in a time- and concentration-dependent manner. After treatment with NVD, the CTL cytotoxicity against SGC-7901 was significantly greater than control. The TNF-α and IL-6 secretion of monocytes and the IgG production of B cells also increased remarkably. Furthermore, NVD could significantly promote the phagocytosis of monocytes on tumor cells. These results suggest that NVD appears to have an immunoenhancing effect on immune cells, indicating that Nidus Vespae is worth exploring for immunomodulatory effects in tumor treatment.

No MeSH data available.


Related in: MedlinePlus

Effects of NVD on monocytes proliferation, tumor cells inhibition, and phagocytosis of monocytes. The monocytes phagocytosis of monocytes + NVD + SGC-7901 group was significantly stronger than that of monocytes + SGC-7901 group, and there were no significant effects of NVD on monocytes proliferation nor on tumor cells inhibition. Data were presented as mean and standard deviation (n = 10).  ∗Significantly different from monocytes + SGC-7901 group (P < 0.0001).
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fig6: Effects of NVD on monocytes proliferation, tumor cells inhibition, and phagocytosis of monocytes. The monocytes phagocytosis of monocytes + NVD + SGC-7901 group was significantly stronger than that of monocytes + SGC-7901 group, and there were no significant effects of NVD on monocytes proliferation nor on tumor cells inhibition. Data were presented as mean and standard deviation (n = 10).  ∗Significantly different from monocytes + SGC-7901 group (P < 0.0001).

Mentions: As shown in Figure 6, there was no significant effect of NVD on monocytes proliferation, nor on tumor cells inhibition. However, there was a marked decrease of OD values in the monocytes + NVD + SGC-7901 group, as compared with the monocytes + SGC-7901 group (P < 0.0001). It indicated that NVD significantly promoted the phagocytosis of monocytes on tumor cells.


The Immunomodulatory Effects of Nidus Vespae on Human Peripheral Blood Immune Cells In Vitro.

Zhu M, Ling Y, Qi Q, Zhang Y, Bao Y, Liu Y - Evid Based Complement Alternat Med (2015)

Effects of NVD on monocytes proliferation, tumor cells inhibition, and phagocytosis of monocytes. The monocytes phagocytosis of monocytes + NVD + SGC-7901 group was significantly stronger than that of monocytes + SGC-7901 group, and there were no significant effects of NVD on monocytes proliferation nor on tumor cells inhibition. Data were presented as mean and standard deviation (n = 10).  ∗Significantly different from monocytes + SGC-7901 group (P < 0.0001).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4539065&req=5

fig6: Effects of NVD on monocytes proliferation, tumor cells inhibition, and phagocytosis of monocytes. The monocytes phagocytosis of monocytes + NVD + SGC-7901 group was significantly stronger than that of monocytes + SGC-7901 group, and there were no significant effects of NVD on monocytes proliferation nor on tumor cells inhibition. Data were presented as mean and standard deviation (n = 10).  ∗Significantly different from monocytes + SGC-7901 group (P < 0.0001).
Mentions: As shown in Figure 6, there was no significant effect of NVD on monocytes proliferation, nor on tumor cells inhibition. However, there was a marked decrease of OD values in the monocytes + NVD + SGC-7901 group, as compared with the monocytes + SGC-7901 group (P < 0.0001). It indicated that NVD significantly promoted the phagocytosis of monocytes on tumor cells.

Bottom Line: Data showed that NVD has no significant inhibiting effects on gastric cancer cells growth.Nevertheless, it could obviously promote PBMC proliferation in a time- and concentration-dependent manner.After treatment with NVD, the CTL cytotoxicity against SGC-7901 was significantly greater than control.

View Article: PubMed Central - PubMed

Affiliation: Clinical Oncology Laboratory, Changzhou Tumor Hospital Affiliated to Soochow University, Huaide Road 44, Changzhou 213002, China.

ABSTRACT
Nidus Vespae has been used in traditional Chinese medicine (TCM) to treat various cancers, but the underlying mechanisms were not yet clarified. This study was to investigate the effect of Nidus Vespae decoction (NVD) on tumor cell viability and immunoregulating functions of human peripheral blood immune cells. The effects on tumor cell viability, peripheral blood mononuclear cell (PBMC) proliferation activity, and the tumor cell phagocytosis of monocytes were evaluated by cell counting kit-8. Tumor-killing activity of cytotoxic T lymphocyte (CTL) was analyzed by (51)Cr releasing assay. IgG production of B cells and cytokine (TNF-α and IL-6) secretion of monocytes were determined by ELISA method. Data showed that NVD has no significant inhibiting effects on gastric cancer cells growth. Nevertheless, it could obviously promote PBMC proliferation in a time- and concentration-dependent manner. After treatment with NVD, the CTL cytotoxicity against SGC-7901 was significantly greater than control. The TNF-α and IL-6 secretion of monocytes and the IgG production of B cells also increased remarkably. Furthermore, NVD could significantly promote the phagocytosis of monocytes on tumor cells. These results suggest that NVD appears to have an immunoenhancing effect on immune cells, indicating that Nidus Vespae is worth exploring for immunomodulatory effects in tumor treatment.

No MeSH data available.


Related in: MedlinePlus