Limits...
The Immunomodulatory Effects of Nidus Vespae on Human Peripheral Blood Immune Cells In Vitro.

Zhu M, Ling Y, Qi Q, Zhang Y, Bao Y, Liu Y - Evid Based Complement Alternat Med (2015)

Bottom Line: Data showed that NVD has no significant inhibiting effects on gastric cancer cells growth.Nevertheless, it could obviously promote PBMC proliferation in a time- and concentration-dependent manner.After treatment with NVD, the CTL cytotoxicity against SGC-7901 was significantly greater than control.

View Article: PubMed Central - PubMed

Affiliation: Clinical Oncology Laboratory, Changzhou Tumor Hospital Affiliated to Soochow University, Huaide Road 44, Changzhou 213002, China.

ABSTRACT
Nidus Vespae has been used in traditional Chinese medicine (TCM) to treat various cancers, but the underlying mechanisms were not yet clarified. This study was to investigate the effect of Nidus Vespae decoction (NVD) on tumor cell viability and immunoregulating functions of human peripheral blood immune cells. The effects on tumor cell viability, peripheral blood mononuclear cell (PBMC) proliferation activity, and the tumor cell phagocytosis of monocytes were evaluated by cell counting kit-8. Tumor-killing activity of cytotoxic T lymphocyte (CTL) was analyzed by (51)Cr releasing assay. IgG production of B cells and cytokine (TNF-α and IL-6) secretion of monocytes were determined by ELISA method. Data showed that NVD has no significant inhibiting effects on gastric cancer cells growth. Nevertheless, it could obviously promote PBMC proliferation in a time- and concentration-dependent manner. After treatment with NVD, the CTL cytotoxicity against SGC-7901 was significantly greater than control. The TNF-α and IL-6 secretion of monocytes and the IgG production of B cells also increased remarkably. Furthermore, NVD could significantly promote the phagocytosis of monocytes on tumor cells. These results suggest that NVD appears to have an immunoenhancing effect on immune cells, indicating that Nidus Vespae is worth exploring for immunomodulatory effects in tumor treatment.

No MeSH data available.


Related in: MedlinePlus

Proliferation rate (%) of PBMC after incubation with Nidus Vespae (75, 150, 300, 600, 1250, 2500, and 5000 μg/mL), control, or ConA (5 μg/mL) for 24 h, 48 h, or 72 h. Data were analyzed by two-way ANOVA and presented as mean and standard deviation (n = 5). It showed that NVD significantly promotes the proliferation of PBMC in a concentration- and time-dependent manner.  ∗Significantly different from PBS control groups (P < 0.001).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4539065&req=5

fig2: Proliferation rate (%) of PBMC after incubation with Nidus Vespae (75, 150, 300, 600, 1250, 2500, and 5000 μg/mL), control, or ConA (5 μg/mL) for 24 h, 48 h, or 72 h. Data were analyzed by two-way ANOVA and presented as mean and standard deviation (n = 5). It showed that NVD significantly promotes the proliferation of PBMC in a concentration- and time-dependent manner.  ∗Significantly different from PBS control groups (P < 0.001).

Mentions: PBMC were incubated with and without NVD at different concentrations. In order to find the optimal concentration of NVD, it was tested at seven concentration levels of 75, 150, 300, 600, 1250, 2500, and 5000 μg/mL at 24 h, 48 h, and 72 h. Compared to the PBS control groups, PBMC promotion was observed very significantly (P < 0.0001) at NVD concentration levels of 600, 1250, 2500, and 5000 μg/mL and to a lesser extent (P < 0.001) at 150 and 300 μg/mL. The lowest concentration of NVD (75 μg/mL) did not show any promotion. Furthermore, the promotion of NVD on PBMC proliferation displayed significant time-dependence (P < 0.001) (Figure 2).


The Immunomodulatory Effects of Nidus Vespae on Human Peripheral Blood Immune Cells In Vitro.

Zhu M, Ling Y, Qi Q, Zhang Y, Bao Y, Liu Y - Evid Based Complement Alternat Med (2015)

Proliferation rate (%) of PBMC after incubation with Nidus Vespae (75, 150, 300, 600, 1250, 2500, and 5000 μg/mL), control, or ConA (5 μg/mL) for 24 h, 48 h, or 72 h. Data were analyzed by two-way ANOVA and presented as mean and standard deviation (n = 5). It showed that NVD significantly promotes the proliferation of PBMC in a concentration- and time-dependent manner.  ∗Significantly different from PBS control groups (P < 0.001).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4539065&req=5

fig2: Proliferation rate (%) of PBMC after incubation with Nidus Vespae (75, 150, 300, 600, 1250, 2500, and 5000 μg/mL), control, or ConA (5 μg/mL) for 24 h, 48 h, or 72 h. Data were analyzed by two-way ANOVA and presented as mean and standard deviation (n = 5). It showed that NVD significantly promotes the proliferation of PBMC in a concentration- and time-dependent manner.  ∗Significantly different from PBS control groups (P < 0.001).
Mentions: PBMC were incubated with and without NVD at different concentrations. In order to find the optimal concentration of NVD, it was tested at seven concentration levels of 75, 150, 300, 600, 1250, 2500, and 5000 μg/mL at 24 h, 48 h, and 72 h. Compared to the PBS control groups, PBMC promotion was observed very significantly (P < 0.0001) at NVD concentration levels of 600, 1250, 2500, and 5000 μg/mL and to a lesser extent (P < 0.001) at 150 and 300 μg/mL. The lowest concentration of NVD (75 μg/mL) did not show any promotion. Furthermore, the promotion of NVD on PBMC proliferation displayed significant time-dependence (P < 0.001) (Figure 2).

Bottom Line: Data showed that NVD has no significant inhibiting effects on gastric cancer cells growth.Nevertheless, it could obviously promote PBMC proliferation in a time- and concentration-dependent manner.After treatment with NVD, the CTL cytotoxicity against SGC-7901 was significantly greater than control.

View Article: PubMed Central - PubMed

Affiliation: Clinical Oncology Laboratory, Changzhou Tumor Hospital Affiliated to Soochow University, Huaide Road 44, Changzhou 213002, China.

ABSTRACT
Nidus Vespae has been used in traditional Chinese medicine (TCM) to treat various cancers, but the underlying mechanisms were not yet clarified. This study was to investigate the effect of Nidus Vespae decoction (NVD) on tumor cell viability and immunoregulating functions of human peripheral blood immune cells. The effects on tumor cell viability, peripheral blood mononuclear cell (PBMC) proliferation activity, and the tumor cell phagocytosis of monocytes were evaluated by cell counting kit-8. Tumor-killing activity of cytotoxic T lymphocyte (CTL) was analyzed by (51)Cr releasing assay. IgG production of B cells and cytokine (TNF-α and IL-6) secretion of monocytes were determined by ELISA method. Data showed that NVD has no significant inhibiting effects on gastric cancer cells growth. Nevertheless, it could obviously promote PBMC proliferation in a time- and concentration-dependent manner. After treatment with NVD, the CTL cytotoxicity against SGC-7901 was significantly greater than control. The TNF-α and IL-6 secretion of monocytes and the IgG production of B cells also increased remarkably. Furthermore, NVD could significantly promote the phagocytosis of monocytes on tumor cells. These results suggest that NVD appears to have an immunoenhancing effect on immune cells, indicating that Nidus Vespae is worth exploring for immunomodulatory effects in tumor treatment.

No MeSH data available.


Related in: MedlinePlus