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Fast Differential Analysis of Propolis Using Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry.

Huang XY, Guo XL, Luo HL, Fang XW, Zhu TG, Zhang XL, Chen HW, Luo LP - Int J Anal Chem (2015)

Bottom Line: Under the optimized experimental conditions, the most abundant signals were detected in the mass ranges of 70 to 500 m/z and 200 to 350 m/z, respectively.Principal component analyses (PCA) for the two mass ranges showed similarities in that the colors had a significant correlation with the first two PCs; in contrast there was no correlation with the climatic zones from which the samples originated.Therefore, SDAPCI-MS can be used for rapid and reliable high-throughput analysis of propolis.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, Nanchang University, Nanchang, Jiangxi 330031, China.

ABSTRACT
Mass spectral fingerprints of 24 raw propolis samples, including 23 from China and one from the United States, were directly obtained using surface desorption atmospheric pressure chemical ionization mass spectrometry (SDAPCI-MS) without sample pretreatment. Under the optimized experimental conditions, the most abundant signals were detected in the mass ranges of 70 to 500 m/z and 200 to 350 m/z, respectively. Principal component analyses (PCA) for the two mass ranges showed similarities in that the colors had a significant correlation with the first two PCs; in contrast there was no correlation with the climatic zones from which the samples originated. Analytes such as chrysin, pinocembrin, and quercetin were detected and identified using multiple stage mass spectrometry within 3 min. Therefore, SDAPCI-MS can be used for rapid and reliable high-throughput analysis of propolis.

No MeSH data available.


Related in: MedlinePlus

PCA analysis of SDAPCI-MS fingerprints. PCA loading plots (ions) for 70–500 m/z (a) and 200–350 m/z (d). PCA score plots for SDAPCI-MS fingerprints of propolis and their colors for 70–500 m/z (b) and 200–350 m/z (e). PCA score plots and their climatic zones for 70–500 m/z (c) and 200–350 m/z (f). For (a) and (d), the numbers of spots mean the m/z of the ions. For (b), (c), (e), and (f), the numbers are their sample number. See Table 1 for characteristics of the samples.
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fig2: PCA analysis of SDAPCI-MS fingerprints. PCA loading plots (ions) for 70–500 m/z (a) and 200–350 m/z (d). PCA score plots for SDAPCI-MS fingerprints of propolis and their colors for 70–500 m/z (b) and 200–350 m/z (e). PCA score plots and their climatic zones for 70–500 m/z (c) and 200–350 m/z (f). For (a) and (d), the numbers of spots mean the m/z of the ions. For (b), (c), (e), and (f), the numbers are their sample number. See Table 1 for characteristics of the samples.

Mentions: Figure 2(a) shows the associations between each of the abundant ions (78, 92, 93, 94, 102, 121, 151, 154, and 183 m/z) and the first two PCs. Clearly, the propolis samples were classified into three groups. Group 1 contained sample numbers 11, 13, 17, 19, 22, and 23; group 2 contained samples 2, 6, 7, 14, 16, 18, and 24; and the rest formed group 3 (Figure 2(b)). As shown by our previous studies, the color of propolis was significantly associated with its quality significantly [12]. Therefore, we tried to reveal the relation between the fingerprints and the colors of Chinese propolis. We noted that most black propolis samples, except sample 1 (HLJ), belonged to group 1; and most yellow propolis samples, except sample 5 (HB-2), belonged to group 2; the other samples, including all the greenish-black and the yellow-green propolis samples belonged to group 3 (Figure 2(b)). These results suggested a significant correlation between the color of propolis samples and PC1 and PC2. A similar correlation was not observed between the climatic zone from which the propolis samples originated and the first two PCs (Figure 2(c)).


Fast Differential Analysis of Propolis Using Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry.

Huang XY, Guo XL, Luo HL, Fang XW, Zhu TG, Zhang XL, Chen HW, Luo LP - Int J Anal Chem (2015)

PCA analysis of SDAPCI-MS fingerprints. PCA loading plots (ions) for 70–500 m/z (a) and 200–350 m/z (d). PCA score plots for SDAPCI-MS fingerprints of propolis and their colors for 70–500 m/z (b) and 200–350 m/z (e). PCA score plots and their climatic zones for 70–500 m/z (c) and 200–350 m/z (f). For (a) and (d), the numbers of spots mean the m/z of the ions. For (b), (c), (e), and (f), the numbers are their sample number. See Table 1 for characteristics of the samples.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig2: PCA analysis of SDAPCI-MS fingerprints. PCA loading plots (ions) for 70–500 m/z (a) and 200–350 m/z (d). PCA score plots for SDAPCI-MS fingerprints of propolis and their colors for 70–500 m/z (b) and 200–350 m/z (e). PCA score plots and their climatic zones for 70–500 m/z (c) and 200–350 m/z (f). For (a) and (d), the numbers of spots mean the m/z of the ions. For (b), (c), (e), and (f), the numbers are their sample number. See Table 1 for characteristics of the samples.
Mentions: Figure 2(a) shows the associations between each of the abundant ions (78, 92, 93, 94, 102, 121, 151, 154, and 183 m/z) and the first two PCs. Clearly, the propolis samples were classified into three groups. Group 1 contained sample numbers 11, 13, 17, 19, 22, and 23; group 2 contained samples 2, 6, 7, 14, 16, 18, and 24; and the rest formed group 3 (Figure 2(b)). As shown by our previous studies, the color of propolis was significantly associated with its quality significantly [12]. Therefore, we tried to reveal the relation between the fingerprints and the colors of Chinese propolis. We noted that most black propolis samples, except sample 1 (HLJ), belonged to group 1; and most yellow propolis samples, except sample 5 (HB-2), belonged to group 2; the other samples, including all the greenish-black and the yellow-green propolis samples belonged to group 3 (Figure 2(b)). These results suggested a significant correlation between the color of propolis samples and PC1 and PC2. A similar correlation was not observed between the climatic zone from which the propolis samples originated and the first two PCs (Figure 2(c)).

Bottom Line: Under the optimized experimental conditions, the most abundant signals were detected in the mass ranges of 70 to 500 m/z and 200 to 350 m/z, respectively.Principal component analyses (PCA) for the two mass ranges showed similarities in that the colors had a significant correlation with the first two PCs; in contrast there was no correlation with the climatic zones from which the samples originated.Therefore, SDAPCI-MS can be used for rapid and reliable high-throughput analysis of propolis.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, Nanchang University, Nanchang, Jiangxi 330031, China.

ABSTRACT
Mass spectral fingerprints of 24 raw propolis samples, including 23 from China and one from the United States, were directly obtained using surface desorption atmospheric pressure chemical ionization mass spectrometry (SDAPCI-MS) without sample pretreatment. Under the optimized experimental conditions, the most abundant signals were detected in the mass ranges of 70 to 500 m/z and 200 to 350 m/z, respectively. Principal component analyses (PCA) for the two mass ranges showed similarities in that the colors had a significant correlation with the first two PCs; in contrast there was no correlation with the climatic zones from which the samples originated. Analytes such as chrysin, pinocembrin, and quercetin were detected and identified using multiple stage mass spectrometry within 3 min. Therefore, SDAPCI-MS can be used for rapid and reliable high-throughput analysis of propolis.

No MeSH data available.


Related in: MedlinePlus