Limits...
Fast Differential Analysis of Propolis Using Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry.

Huang XY, Guo XL, Luo HL, Fang XW, Zhu TG, Zhang XL, Chen HW, Luo LP - Int J Anal Chem (2015)

Bottom Line: Under the optimized experimental conditions, the most abundant signals were detected in the mass ranges of 70 to 500 m/z and 200 to 350 m/z, respectively.Principal component analyses (PCA) for the two mass ranges showed similarities in that the colors had a significant correlation with the first two PCs; in contrast there was no correlation with the climatic zones from which the samples originated.Therefore, SDAPCI-MS can be used for rapid and reliable high-throughput analysis of propolis.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, Nanchang University, Nanchang, Jiangxi 330031, China.

ABSTRACT
Mass spectral fingerprints of 24 raw propolis samples, including 23 from China and one from the United States, were directly obtained using surface desorption atmospheric pressure chemical ionization mass spectrometry (SDAPCI-MS) without sample pretreatment. Under the optimized experimental conditions, the most abundant signals were detected in the mass ranges of 70 to 500 m/z and 200 to 350 m/z, respectively. Principal component analyses (PCA) for the two mass ranges showed similarities in that the colors had a significant correlation with the first two PCs; in contrast there was no correlation with the climatic zones from which the samples originated. Analytes such as chrysin, pinocembrin, and quercetin were detected and identified using multiple stage mass spectrometry within 3 min. Therefore, SDAPCI-MS can be used for rapid and reliable high-throughput analysis of propolis.

No MeSH data available.


Schematic diagram of SDAPCI source and optimization of SDAPCI source conditions. (a) Schematic diagram of SDAPCI source for measurement of propolis. (b)–(e) Optimization of SDAPCI source conditions including the effect of discharge voltage on signal intensity (b), the effect of the angles α (c), the effect of the distance between the discharge tip and the ion entrance (d), and the effect of the temperature of the heated capillary (e). All the optimization experiments were based on the signal intensity of the peak signal at 121 m/z for sample 1 (HLJ). Each point represents an average of six measurements.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4539062&req=5

fig1: Schematic diagram of SDAPCI source and optimization of SDAPCI source conditions. (a) Schematic diagram of SDAPCI source for measurement of propolis. (b)–(e) Optimization of SDAPCI source conditions including the effect of discharge voltage on signal intensity (b), the effect of the angles α (c), the effect of the distance between the discharge tip and the ion entrance (d), and the effect of the temperature of the heated capillary (e). All the optimization experiments were based on the signal intensity of the peak signal at 121 m/z for sample 1 (HLJ). Each point represents an average of six measurements.

Mentions: A schematic diagram of the SDAPCI source is shown in Figure 1(a). The principle and the experimental setup for the SDAPCI have been described previously [32]. A cylindrical electrode with a cone on one end was secured by an insulator of 5 mm length exposed to the air. The LTQ-MS system was set for negative ion mode detection and the mass scan range was 65–1000 m/z; the voltage of the discharge needle electrode was 3.5 kV, and the temperature of the capillary of the LTQ instrument was maintained at 275°C; the parent ions of interest were selected with a mass-to-charge window of 1.4 units; the collision-induced dissociation (CID) experiments were performed with 10–30 units of collision energy (CE) and 30 ms duration; all of the full-scan mass spectra were collected with an average time of 1 min and with background subtraction. Other parameters were optimized automatically by the LTQ-MS system. The distance between the discharge needle tip and the ion entrance was 10 mm and the distance between the discharge needle tip and the sample surface was 2 mm. The angle between the discharge needle and the sample surface was 30°, and the angle formed by the ion entrance capillary and the sample holder was 25°.


Fast Differential Analysis of Propolis Using Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry.

Huang XY, Guo XL, Luo HL, Fang XW, Zhu TG, Zhang XL, Chen HW, Luo LP - Int J Anal Chem (2015)

Schematic diagram of SDAPCI source and optimization of SDAPCI source conditions. (a) Schematic diagram of SDAPCI source for measurement of propolis. (b)–(e) Optimization of SDAPCI source conditions including the effect of discharge voltage on signal intensity (b), the effect of the angles α (c), the effect of the distance between the discharge tip and the ion entrance (d), and the effect of the temperature of the heated capillary (e). All the optimization experiments were based on the signal intensity of the peak signal at 121 m/z for sample 1 (HLJ). Each point represents an average of six measurements.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4539062&req=5

fig1: Schematic diagram of SDAPCI source and optimization of SDAPCI source conditions. (a) Schematic diagram of SDAPCI source for measurement of propolis. (b)–(e) Optimization of SDAPCI source conditions including the effect of discharge voltage on signal intensity (b), the effect of the angles α (c), the effect of the distance between the discharge tip and the ion entrance (d), and the effect of the temperature of the heated capillary (e). All the optimization experiments were based on the signal intensity of the peak signal at 121 m/z for sample 1 (HLJ). Each point represents an average of six measurements.
Mentions: A schematic diagram of the SDAPCI source is shown in Figure 1(a). The principle and the experimental setup for the SDAPCI have been described previously [32]. A cylindrical electrode with a cone on one end was secured by an insulator of 5 mm length exposed to the air. The LTQ-MS system was set for negative ion mode detection and the mass scan range was 65–1000 m/z; the voltage of the discharge needle electrode was 3.5 kV, and the temperature of the capillary of the LTQ instrument was maintained at 275°C; the parent ions of interest were selected with a mass-to-charge window of 1.4 units; the collision-induced dissociation (CID) experiments were performed with 10–30 units of collision energy (CE) and 30 ms duration; all of the full-scan mass spectra were collected with an average time of 1 min and with background subtraction. Other parameters were optimized automatically by the LTQ-MS system. The distance between the discharge needle tip and the ion entrance was 10 mm and the distance between the discharge needle tip and the sample surface was 2 mm. The angle between the discharge needle and the sample surface was 30°, and the angle formed by the ion entrance capillary and the sample holder was 25°.

Bottom Line: Under the optimized experimental conditions, the most abundant signals were detected in the mass ranges of 70 to 500 m/z and 200 to 350 m/z, respectively.Principal component analyses (PCA) for the two mass ranges showed similarities in that the colors had a significant correlation with the first two PCs; in contrast there was no correlation with the climatic zones from which the samples originated.Therefore, SDAPCI-MS can be used for rapid and reliable high-throughput analysis of propolis.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, Nanchang University, Nanchang, Jiangxi 330031, China.

ABSTRACT
Mass spectral fingerprints of 24 raw propolis samples, including 23 from China and one from the United States, were directly obtained using surface desorption atmospheric pressure chemical ionization mass spectrometry (SDAPCI-MS) without sample pretreatment. Under the optimized experimental conditions, the most abundant signals were detected in the mass ranges of 70 to 500 m/z and 200 to 350 m/z, respectively. Principal component analyses (PCA) for the two mass ranges showed similarities in that the colors had a significant correlation with the first two PCs; in contrast there was no correlation with the climatic zones from which the samples originated. Analytes such as chrysin, pinocembrin, and quercetin were detected and identified using multiple stage mass spectrometry within 3 min. Therefore, SDAPCI-MS can be used for rapid and reliable high-throughput analysis of propolis.

No MeSH data available.