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Herb-Partitioned Moxibustion Regulates the TLR2/NF-κB Signaling Pathway in a Rat Model of Ulcerative Colitis.

Wang X, Liu Y, Dong H, Wu L, Feng X, Zhou Z, Zhao C, Liu H, Wu H - Evid Based Complement Alternat Med (2015)

Bottom Line: This study observed the effect of moxibustion on the TLR2/NF-κB signaling pathway at the Tianshu (ST25) and Qihai (CV6) acupuncture points in the UC rat.Similarly, the expression of NF-κB was significantly increased and IFN-β and IL-10 were significantly decreased in the colonic mucosa of UC rats, but herb-partitioned moxibustion reduced the expression of IFN-β and upregulating the expression of IFN-β and IL-10 significantly.It indicates that herb-partitioned moxibustion can inhibit the expression of multiple signaling molecules of the TLR2 pathway effectively, and it may modulate the excessive local immune response by inhibiting TLR2 signaling, thereby promoting the repair of damaged colonic mucosa.

View Article: PubMed Central - PubMed

Affiliation: Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

ABSTRACT
The TLR2/NF-κB signaling pathway plays an important role in the pathomechanism of ulcerative colitis (UC); acupuncture and moxibustion can improve the damage in colonic tissues of UC, but the regulatory mechanism remains unknown. This study observed the effect of moxibustion on the TLR2/NF-κB signaling pathway at the Tianshu (ST25) and Qihai (CV6) acupuncture points in the UC rat. The result shows that TLR2, IRAK1, and IKK-b mRNA and protein levels in the colonic mucosa were significantly higher in the UC rats than in the control rats. Herb-partitioned moxibustion reduced the expression of TLR2, IRAK1, and IKK-b mRNA and proteins in the UC rats. Similarly, the expression of NF-κB was significantly increased and IFN-β and IL-10 were significantly decreased in the colonic mucosa of UC rats, but herb-partitioned moxibustion reduced the expression of IFN-β and upregulating the expression of IFN-β and IL-10 significantly. It indicates that herb-partitioned moxibustion can inhibit the expression of multiple signaling molecules of the TLR2 pathway effectively, and it may modulate the excessive local immune response by inhibiting TLR2 signaling, thereby promoting the repair of damaged colonic mucosa.

No MeSH data available.


Related in: MedlinePlus

Expression of IL-10 protein in the colonic mucosa of rat. NC: normal control; MC: ulcerative colitis; HPM: UC with herb-partitioned moxibustion; SA: UC with salicylazosulfapyridine. #P < 0.01 versus NC; ∗P < 0.01 versus MC.
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fig16: Expression of IL-10 protein in the colonic mucosa of rat. NC: normal control; MC: ulcerative colitis; HPM: UC with herb-partitioned moxibustion; SA: UC with salicylazosulfapyridine. #P < 0.01 versus NC; ∗P < 0.01 versus MC.

Mentions: As shown in Figures 16 and 17, IL-10 protein levels in the colonic mucosa were significantly lower in UC rats than in the control rats, as indicated by both the total area of staining and the integrated optical density (P < 0.05 in both cases). Both herb-partitioned moxibustion and SASP treatments significantly increased IL-10 protein levels in the colonic mucosa of UC rats by both of these measurements (P < 0.05 in all cases). The total area of IL-10 staining in the colonic mucosa was significantly higher in the herb-partitioned moxibustion group than in the SASP group (P < 0.05) but the integrated optical density did not reach statistical significance, although it was higher in the herb-partitioned moxibustion group than in the SASP group (P > 0.05).


Herb-Partitioned Moxibustion Regulates the TLR2/NF-κB Signaling Pathway in a Rat Model of Ulcerative Colitis.

Wang X, Liu Y, Dong H, Wu L, Feng X, Zhou Z, Zhao C, Liu H, Wu H - Evid Based Complement Alternat Med (2015)

Expression of IL-10 protein in the colonic mucosa of rat. NC: normal control; MC: ulcerative colitis; HPM: UC with herb-partitioned moxibustion; SA: UC with salicylazosulfapyridine. #P < 0.01 versus NC; ∗P < 0.01 versus MC.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538972&req=5

fig16: Expression of IL-10 protein in the colonic mucosa of rat. NC: normal control; MC: ulcerative colitis; HPM: UC with herb-partitioned moxibustion; SA: UC with salicylazosulfapyridine. #P < 0.01 versus NC; ∗P < 0.01 versus MC.
Mentions: As shown in Figures 16 and 17, IL-10 protein levels in the colonic mucosa were significantly lower in UC rats than in the control rats, as indicated by both the total area of staining and the integrated optical density (P < 0.05 in both cases). Both herb-partitioned moxibustion and SASP treatments significantly increased IL-10 protein levels in the colonic mucosa of UC rats by both of these measurements (P < 0.05 in all cases). The total area of IL-10 staining in the colonic mucosa was significantly higher in the herb-partitioned moxibustion group than in the SASP group (P < 0.05) but the integrated optical density did not reach statistical significance, although it was higher in the herb-partitioned moxibustion group than in the SASP group (P > 0.05).

Bottom Line: This study observed the effect of moxibustion on the TLR2/NF-κB signaling pathway at the Tianshu (ST25) and Qihai (CV6) acupuncture points in the UC rat.Similarly, the expression of NF-κB was significantly increased and IFN-β and IL-10 were significantly decreased in the colonic mucosa of UC rats, but herb-partitioned moxibustion reduced the expression of IFN-β and upregulating the expression of IFN-β and IL-10 significantly.It indicates that herb-partitioned moxibustion can inhibit the expression of multiple signaling molecules of the TLR2 pathway effectively, and it may modulate the excessive local immune response by inhibiting TLR2 signaling, thereby promoting the repair of damaged colonic mucosa.

View Article: PubMed Central - PubMed

Affiliation: Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

ABSTRACT
The TLR2/NF-κB signaling pathway plays an important role in the pathomechanism of ulcerative colitis (UC); acupuncture and moxibustion can improve the damage in colonic tissues of UC, but the regulatory mechanism remains unknown. This study observed the effect of moxibustion on the TLR2/NF-κB signaling pathway at the Tianshu (ST25) and Qihai (CV6) acupuncture points in the UC rat. The result shows that TLR2, IRAK1, and IKK-b mRNA and protein levels in the colonic mucosa were significantly higher in the UC rats than in the control rats. Herb-partitioned moxibustion reduced the expression of TLR2, IRAK1, and IKK-b mRNA and proteins in the UC rats. Similarly, the expression of NF-κB was significantly increased and IFN-β and IL-10 were significantly decreased in the colonic mucosa of UC rats, but herb-partitioned moxibustion reduced the expression of IFN-β and upregulating the expression of IFN-β and IL-10 significantly. It indicates that herb-partitioned moxibustion can inhibit the expression of multiple signaling molecules of the TLR2 pathway effectively, and it may modulate the excessive local immune response by inhibiting TLR2 signaling, thereby promoting the repair of damaged colonic mucosa.

No MeSH data available.


Related in: MedlinePlus