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Herb-Partitioned Moxibustion Regulates the TLR2/NF-κB Signaling Pathway in a Rat Model of Ulcerative Colitis.

Wang X, Liu Y, Dong H, Wu L, Feng X, Zhou Z, Zhao C, Liu H, Wu H - Evid Based Complement Alternat Med (2015)

Bottom Line: This study observed the effect of moxibustion on the TLR2/NF-κB signaling pathway at the Tianshu (ST25) and Qihai (CV6) acupuncture points in the UC rat.Similarly, the expression of NF-κB was significantly increased and IFN-β and IL-10 were significantly decreased in the colonic mucosa of UC rats, but herb-partitioned moxibustion reduced the expression of IFN-β and upregulating the expression of IFN-β and IL-10 significantly.It indicates that herb-partitioned moxibustion can inhibit the expression of multiple signaling molecules of the TLR2 pathway effectively, and it may modulate the excessive local immune response by inhibiting TLR2 signaling, thereby promoting the repair of damaged colonic mucosa.

View Article: PubMed Central - PubMed

Affiliation: Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

ABSTRACT
The TLR2/NF-κB signaling pathway plays an important role in the pathomechanism of ulcerative colitis (UC); acupuncture and moxibustion can improve the damage in colonic tissues of UC, but the regulatory mechanism remains unknown. This study observed the effect of moxibustion on the TLR2/NF-κB signaling pathway at the Tianshu (ST25) and Qihai (CV6) acupuncture points in the UC rat. The result shows that TLR2, IRAK1, and IKK-b mRNA and protein levels in the colonic mucosa were significantly higher in the UC rats than in the control rats. Herb-partitioned moxibustion reduced the expression of TLR2, IRAK1, and IKK-b mRNA and proteins in the UC rats. Similarly, the expression of NF-κB was significantly increased and IFN-β and IL-10 were significantly decreased in the colonic mucosa of UC rats, but herb-partitioned moxibustion reduced the expression of IFN-β and upregulating the expression of IFN-β and IL-10 significantly. It indicates that herb-partitioned moxibustion can inhibit the expression of multiple signaling molecules of the TLR2 pathway effectively, and it may modulate the excessive local immune response by inhibiting TLR2 signaling, thereby promoting the repair of damaged colonic mucosa.

No MeSH data available.


Related in: MedlinePlus

The IRAK-1 mRNA levels in the rat colon of each group. NC: normal control; MC: ulcerative colitis; HPM: UC with herb-partitioned moxibustion; SA: UC with salicylazosulfapyridine. #P < 0.01 versus NC; ∗P < 0.01, △P < 0.05 versus MC; ▲P < 0.01 versus HPM.
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fig6: The IRAK-1 mRNA levels in the rat colon of each group. NC: normal control; MC: ulcerative colitis; HPM: UC with herb-partitioned moxibustion; SA: UC with salicylazosulfapyridine. #P < 0.01 versus NC; ∗P < 0.01, △P < 0.05 versus MC; ▲P < 0.01 versus HPM.

Mentions: As shown in Figure 6, rats in the UC group expressed significantly higher levels of IRAK1 mRNA in the colonic mucosa than rats in the control group (P < 0.01). Both herb-partitioned moxibustion and SASP treatments significantly reduced IRAK-1 mRNA expression in the colonic mucosa of UC rats (P < 0.01, P < 0.05). Between the two treatment groups, IRAK1 mRNA expression in the colonic mucosa was significantly higher with SASP than with herb-partitioned moxibustion treatment (P < 0.01).


Herb-Partitioned Moxibustion Regulates the TLR2/NF-κB Signaling Pathway in a Rat Model of Ulcerative Colitis.

Wang X, Liu Y, Dong H, Wu L, Feng X, Zhou Z, Zhao C, Liu H, Wu H - Evid Based Complement Alternat Med (2015)

The IRAK-1 mRNA levels in the rat colon of each group. NC: normal control; MC: ulcerative colitis; HPM: UC with herb-partitioned moxibustion; SA: UC with salicylazosulfapyridine. #P < 0.01 versus NC; ∗P < 0.01, △P < 0.05 versus MC; ▲P < 0.01 versus HPM.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538972&req=5

fig6: The IRAK-1 mRNA levels in the rat colon of each group. NC: normal control; MC: ulcerative colitis; HPM: UC with herb-partitioned moxibustion; SA: UC with salicylazosulfapyridine. #P < 0.01 versus NC; ∗P < 0.01, △P < 0.05 versus MC; ▲P < 0.01 versus HPM.
Mentions: As shown in Figure 6, rats in the UC group expressed significantly higher levels of IRAK1 mRNA in the colonic mucosa than rats in the control group (P < 0.01). Both herb-partitioned moxibustion and SASP treatments significantly reduced IRAK-1 mRNA expression in the colonic mucosa of UC rats (P < 0.01, P < 0.05). Between the two treatment groups, IRAK1 mRNA expression in the colonic mucosa was significantly higher with SASP than with herb-partitioned moxibustion treatment (P < 0.01).

Bottom Line: This study observed the effect of moxibustion on the TLR2/NF-κB signaling pathway at the Tianshu (ST25) and Qihai (CV6) acupuncture points in the UC rat.Similarly, the expression of NF-κB was significantly increased and IFN-β and IL-10 were significantly decreased in the colonic mucosa of UC rats, but herb-partitioned moxibustion reduced the expression of IFN-β and upregulating the expression of IFN-β and IL-10 significantly.It indicates that herb-partitioned moxibustion can inhibit the expression of multiple signaling molecules of the TLR2 pathway effectively, and it may modulate the excessive local immune response by inhibiting TLR2 signaling, thereby promoting the repair of damaged colonic mucosa.

View Article: PubMed Central - PubMed

Affiliation: Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

ABSTRACT
The TLR2/NF-κB signaling pathway plays an important role in the pathomechanism of ulcerative colitis (UC); acupuncture and moxibustion can improve the damage in colonic tissues of UC, but the regulatory mechanism remains unknown. This study observed the effect of moxibustion on the TLR2/NF-κB signaling pathway at the Tianshu (ST25) and Qihai (CV6) acupuncture points in the UC rat. The result shows that TLR2, IRAK1, and IKK-b mRNA and protein levels in the colonic mucosa were significantly higher in the UC rats than in the control rats. Herb-partitioned moxibustion reduced the expression of TLR2, IRAK1, and IKK-b mRNA and proteins in the UC rats. Similarly, the expression of NF-κB was significantly increased and IFN-β and IL-10 were significantly decreased in the colonic mucosa of UC rats, but herb-partitioned moxibustion reduced the expression of IFN-β and upregulating the expression of IFN-β and IL-10 significantly. It indicates that herb-partitioned moxibustion can inhibit the expression of multiple signaling molecules of the TLR2 pathway effectively, and it may modulate the excessive local immune response by inhibiting TLR2 signaling, thereby promoting the repair of damaged colonic mucosa.

No MeSH data available.


Related in: MedlinePlus