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Herb-Partitioned Moxibustion Regulates the TLR2/NF-κB Signaling Pathway in a Rat Model of Ulcerative Colitis.

Wang X, Liu Y, Dong H, Wu L, Feng X, Zhou Z, Zhao C, Liu H, Wu H - Evid Based Complement Alternat Med (2015)

Bottom Line: This study observed the effect of moxibustion on the TLR2/NF-κB signaling pathway at the Tianshu (ST25) and Qihai (CV6) acupuncture points in the UC rat.Similarly, the expression of NF-κB was significantly increased and IFN-β and IL-10 were significantly decreased in the colonic mucosa of UC rats, but herb-partitioned moxibustion reduced the expression of IFN-β and upregulating the expression of IFN-β and IL-10 significantly.It indicates that herb-partitioned moxibustion can inhibit the expression of multiple signaling molecules of the TLR2 pathway effectively, and it may modulate the excessive local immune response by inhibiting TLR2 signaling, thereby promoting the repair of damaged colonic mucosa.

View Article: PubMed Central - PubMed

Affiliation: Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

ABSTRACT
The TLR2/NF-κB signaling pathway plays an important role in the pathomechanism of ulcerative colitis (UC); acupuncture and moxibustion can improve the damage in colonic tissues of UC, but the regulatory mechanism remains unknown. This study observed the effect of moxibustion on the TLR2/NF-κB signaling pathway at the Tianshu (ST25) and Qihai (CV6) acupuncture points in the UC rat. The result shows that TLR2, IRAK1, and IKK-b mRNA and protein levels in the colonic mucosa were significantly higher in the UC rats than in the control rats. Herb-partitioned moxibustion reduced the expression of TLR2, IRAK1, and IKK-b mRNA and proteins in the UC rats. Similarly, the expression of NF-κB was significantly increased and IFN-β and IL-10 were significantly decreased in the colonic mucosa of UC rats, but herb-partitioned moxibustion reduced the expression of IFN-β and upregulating the expression of IFN-β and IL-10 significantly. It indicates that herb-partitioned moxibustion can inhibit the expression of multiple signaling molecules of the TLR2 pathway effectively, and it may modulate the excessive local immune response by inhibiting TLR2 signaling, thereby promoting the repair of damaged colonic mucosa.

No MeSH data available.


Related in: MedlinePlus

The histological observation of rats in each group. Hematoxylin-Eosin staining method, ×200. (a) Normal control; (b) ulcerative colitis; (c) UC with herb-partitioned moxibustion; (d) UC with salicylazosulfapyridine.
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Related In: Results  -  Collection


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fig2: The histological observation of rats in each group. Hematoxylin-Eosin staining method, ×200. (a) Normal control; (b) ulcerative colitis; (c) UC with herb-partitioned moxibustion; (d) UC with salicylazosulfapyridine.

Mentions: As shown in Figure 2, under a light microscope, normal colonic mucosa had a well-organized structure with intestinal glands arranged in rows and an intact colonic epithelium. Capillaries and scattered lymphocytes were visible in the lamina propria, but there was no significant inflammatory cell infiltration. In the colonic mucosa of UC rats, the crypts were shorter and the epithelium was not intact. The mucosa and submucosa had been infiltrated by a large number of inflammatory cells, focal hyperemia and edema were visible, and the structures of the glands were disorganized, indicating that ulcers had formed. Both herb-partitioned moxibustion and SASP treatments greatly improved the morphology of colonic mucosa. Intestinal glands were more organized than those in UC rats. Ulceration was less severe, and the mucosa surface was covered by epithelial cells. Edema and inflammatory cell infiltration in submucosa were still present, but they were less severe than in UC rats.


Herb-Partitioned Moxibustion Regulates the TLR2/NF-κB Signaling Pathway in a Rat Model of Ulcerative Colitis.

Wang X, Liu Y, Dong H, Wu L, Feng X, Zhou Z, Zhao C, Liu H, Wu H - Evid Based Complement Alternat Med (2015)

The histological observation of rats in each group. Hematoxylin-Eosin staining method, ×200. (a) Normal control; (b) ulcerative colitis; (c) UC with herb-partitioned moxibustion; (d) UC with salicylazosulfapyridine.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538972&req=5

fig2: The histological observation of rats in each group. Hematoxylin-Eosin staining method, ×200. (a) Normal control; (b) ulcerative colitis; (c) UC with herb-partitioned moxibustion; (d) UC with salicylazosulfapyridine.
Mentions: As shown in Figure 2, under a light microscope, normal colonic mucosa had a well-organized structure with intestinal glands arranged in rows and an intact colonic epithelium. Capillaries and scattered lymphocytes were visible in the lamina propria, but there was no significant inflammatory cell infiltration. In the colonic mucosa of UC rats, the crypts were shorter and the epithelium was not intact. The mucosa and submucosa had been infiltrated by a large number of inflammatory cells, focal hyperemia and edema were visible, and the structures of the glands were disorganized, indicating that ulcers had formed. Both herb-partitioned moxibustion and SASP treatments greatly improved the morphology of colonic mucosa. Intestinal glands were more organized than those in UC rats. Ulceration was less severe, and the mucosa surface was covered by epithelial cells. Edema and inflammatory cell infiltration in submucosa were still present, but they were less severe than in UC rats.

Bottom Line: This study observed the effect of moxibustion on the TLR2/NF-κB signaling pathway at the Tianshu (ST25) and Qihai (CV6) acupuncture points in the UC rat.Similarly, the expression of NF-κB was significantly increased and IFN-β and IL-10 were significantly decreased in the colonic mucosa of UC rats, but herb-partitioned moxibustion reduced the expression of IFN-β and upregulating the expression of IFN-β and IL-10 significantly.It indicates that herb-partitioned moxibustion can inhibit the expression of multiple signaling molecules of the TLR2 pathway effectively, and it may modulate the excessive local immune response by inhibiting TLR2 signaling, thereby promoting the repair of damaged colonic mucosa.

View Article: PubMed Central - PubMed

Affiliation: Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

ABSTRACT
The TLR2/NF-κB signaling pathway plays an important role in the pathomechanism of ulcerative colitis (UC); acupuncture and moxibustion can improve the damage in colonic tissues of UC, but the regulatory mechanism remains unknown. This study observed the effect of moxibustion on the TLR2/NF-κB signaling pathway at the Tianshu (ST25) and Qihai (CV6) acupuncture points in the UC rat. The result shows that TLR2, IRAK1, and IKK-b mRNA and protein levels in the colonic mucosa were significantly higher in the UC rats than in the control rats. Herb-partitioned moxibustion reduced the expression of TLR2, IRAK1, and IKK-b mRNA and proteins in the UC rats. Similarly, the expression of NF-κB was significantly increased and IFN-β and IL-10 were significantly decreased in the colonic mucosa of UC rats, but herb-partitioned moxibustion reduced the expression of IFN-β and upregulating the expression of IFN-β and IL-10 significantly. It indicates that herb-partitioned moxibustion can inhibit the expression of multiple signaling molecules of the TLR2 pathway effectively, and it may modulate the excessive local immune response by inhibiting TLR2 signaling, thereby promoting the repair of damaged colonic mucosa.

No MeSH data available.


Related in: MedlinePlus