Limits...
Protein kinase C delta mice exhibit structural alterations in articular surface, intra-articular and subchondral compartments.

Yang X, Teguh D, Wu JP, He B, Kirk TB, Qin S, Li S, Chen H, Xue W, Ng B, Chim SM, Tickner J, Xu J - Arthritis Res. Ther. (2015)

Bottom Line: Structural alterations in intra-articular and subchondral compartments are hallmarks of osteoarthritis, a degenerative disease that causes pain and disability in the aging population.The morphology and organization of chondrocytes were studied using confocal microscopy.Interestingly, loss of demarcation between articular cartilage and bone was concomitant with irregular chondrocyte morphology and arrangement.

View Article: PubMed Central - PubMed

Affiliation: Guangzhou Institute of Traumatic Surgery, the Fourth Affiliated Hospital of Medical College, Jinan University, Guangzhou, 510220, China. dr.yang1192@yahoo.com.

ABSTRACT

Introduction: Structural alterations in intra-articular and subchondral compartments are hallmarks of osteoarthritis, a degenerative disease that causes pain and disability in the aging population. Protein kinase C delta (PKC-δ) plays versatile functions in cell growth and differentiation, but its role in the articular cartilage and subchondral bone is not known.

Methods: Histological analysis including alcian blue, safranin O staining and fluorochrome labeling were used to reveal structural alterations at the articular cartilage surface and bone-cartilage interface in PKC-δ knockout (KO) mice. The morphology and organization of chondrocytes were studied using confocal microscopy. Glycosaminoglycan content was studied by micromass culture of chondrocytes of PKC-δ KO mice.

Results: We uncovered atypical structural demarcation between articular cartilage and subchondral bone of PKC-δ KO mice. Histology analyses revealed a thickening of the articular cartilage and calcified bone-cartilage interface, and decreased safranin O staining accompanied by an increase in the number of hypertrophic chondrocytes in the articular cartilage of PKC-δ KO mice. Interestingly, loss of demarcation between articular cartilage and bone was concomitant with irregular chondrocyte morphology and arrangement. Consistently, in vivo calcein labeling assay showed an increased intensity of calcein labeling in the interface of the growth plate and metaphysis in PKC-δ KO mice. Furthermore, in vitro culture of chondrocyte micromass showed a decreased alcian blue staining of chondrocyte micromass in the PKC-δ KO mice, indicative of a reduced level of glycosaminoglycan production.

Conclusions: Our data imply a role for PKC-δ in the osteochondral plasticity of the interface between articular cartilage and the osteochondral junction.

No MeSH data available.


Related in: MedlinePlus

Confocal microscopic examination of chondrocytes indicated there is a significant morphological difference between the chondrocytes of protein kinase C delta (PKC-δ)+/+ (a, c) and PKC-δ−/− (b, d). a, b Chondrocyte morphology revealed by a MBI reconstructed from the confocal image stacks of PKC-δ+/+ and PKC-δ−/− mice, respectively. c, d Three-dimensional (3D) images reconstructed from the confocal image stacks using Voxblast. (Field of view: 150 μm × 150 μm)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4538913&req=5

Fig2: Confocal microscopic examination of chondrocytes indicated there is a significant morphological difference between the chondrocytes of protein kinase C delta (PKC-δ)+/+ (a, c) and PKC-δ−/− (b, d). a, b Chondrocyte morphology revealed by a MBI reconstructed from the confocal image stacks of PKC-δ+/+ and PKC-δ−/− mice, respectively. c, d Three-dimensional (3D) images reconstructed from the confocal image stacks using Voxblast. (Field of view: 150 μm × 150 μm)

Mentions: Histological analysis further revealed an increased number of hypertrophic chondrocytes with irregular chondrocyte arrangement in PKC-δ KO mice (Fig. 1b,d). Strikingly, using FOLSCM analysis, we further observed alterations in cell morphology and arrangement of chondrocytes in PKC-δ KO mice (Fig. 2b,d) in comparison with the WT mice (Fig. 2a,c). The chondrocytes in the PKC-δ KO mice display irregular shapes as compared to those in WT articular cartilage. In addition, there is also an apparent reduction in the numbers of chondrocytes in the PKC-δ KO mice.Fig. 2


Protein kinase C delta mice exhibit structural alterations in articular surface, intra-articular and subchondral compartments.

Yang X, Teguh D, Wu JP, He B, Kirk TB, Qin S, Li S, Chen H, Xue W, Ng B, Chim SM, Tickner J, Xu J - Arthritis Res. Ther. (2015)

Confocal microscopic examination of chondrocytes indicated there is a significant morphological difference between the chondrocytes of protein kinase C delta (PKC-δ)+/+ (a, c) and PKC-δ−/− (b, d). a, b Chondrocyte morphology revealed by a MBI reconstructed from the confocal image stacks of PKC-δ+/+ and PKC-δ−/− mice, respectively. c, d Three-dimensional (3D) images reconstructed from the confocal image stacks using Voxblast. (Field of view: 150 μm × 150 μm)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4538913&req=5

Fig2: Confocal microscopic examination of chondrocytes indicated there is a significant morphological difference between the chondrocytes of protein kinase C delta (PKC-δ)+/+ (a, c) and PKC-δ−/− (b, d). a, b Chondrocyte morphology revealed by a MBI reconstructed from the confocal image stacks of PKC-δ+/+ and PKC-δ−/− mice, respectively. c, d Three-dimensional (3D) images reconstructed from the confocal image stacks using Voxblast. (Field of view: 150 μm × 150 μm)
Mentions: Histological analysis further revealed an increased number of hypertrophic chondrocytes with irregular chondrocyte arrangement in PKC-δ KO mice (Fig. 1b,d). Strikingly, using FOLSCM analysis, we further observed alterations in cell morphology and arrangement of chondrocytes in PKC-δ KO mice (Fig. 2b,d) in comparison with the WT mice (Fig. 2a,c). The chondrocytes in the PKC-δ KO mice display irregular shapes as compared to those in WT articular cartilage. In addition, there is also an apparent reduction in the numbers of chondrocytes in the PKC-δ KO mice.Fig. 2

Bottom Line: Structural alterations in intra-articular and subchondral compartments are hallmarks of osteoarthritis, a degenerative disease that causes pain and disability in the aging population.The morphology and organization of chondrocytes were studied using confocal microscopy.Interestingly, loss of demarcation between articular cartilage and bone was concomitant with irregular chondrocyte morphology and arrangement.

View Article: PubMed Central - PubMed

Affiliation: Guangzhou Institute of Traumatic Surgery, the Fourth Affiliated Hospital of Medical College, Jinan University, Guangzhou, 510220, China. dr.yang1192@yahoo.com.

ABSTRACT

Introduction: Structural alterations in intra-articular and subchondral compartments are hallmarks of osteoarthritis, a degenerative disease that causes pain and disability in the aging population. Protein kinase C delta (PKC-δ) plays versatile functions in cell growth and differentiation, but its role in the articular cartilage and subchondral bone is not known.

Methods: Histological analysis including alcian blue, safranin O staining and fluorochrome labeling were used to reveal structural alterations at the articular cartilage surface and bone-cartilage interface in PKC-δ knockout (KO) mice. The morphology and organization of chondrocytes were studied using confocal microscopy. Glycosaminoglycan content was studied by micromass culture of chondrocytes of PKC-δ KO mice.

Results: We uncovered atypical structural demarcation between articular cartilage and subchondral bone of PKC-δ KO mice. Histology analyses revealed a thickening of the articular cartilage and calcified bone-cartilage interface, and decreased safranin O staining accompanied by an increase in the number of hypertrophic chondrocytes in the articular cartilage of PKC-δ KO mice. Interestingly, loss of demarcation between articular cartilage and bone was concomitant with irregular chondrocyte morphology and arrangement. Consistently, in vivo calcein labeling assay showed an increased intensity of calcein labeling in the interface of the growth plate and metaphysis in PKC-δ KO mice. Furthermore, in vitro culture of chondrocyte micromass showed a decreased alcian blue staining of chondrocyte micromass in the PKC-δ KO mice, indicative of a reduced level of glycosaminoglycan production.

Conclusions: Our data imply a role for PKC-δ in the osteochondral plasticity of the interface between articular cartilage and the osteochondral junction.

No MeSH data available.


Related in: MedlinePlus