Species-Level Phylogeny and Polyploid Relationships in Hordeum (Poaceae) Inferred by Next-Generation Sequencing and In Silico Cloning of Multiple Nuclear Loci.
Bottom Line: For diploid taxa, a Bayesian concordance analysis and a coalescent-based dated species tree was inferred from all gene trees.The resulting multilocus phylogeny reveals for the first time species phylogeny and progenitor-derivative relationships of all di- and polyploid Hordeum taxa within a single analysis.Our study proves that it is possible to obtain a multilocus species-level phylogeny for di- and polyploid taxa by combining PCR with next-generation sequencing, without cloning and without creating a heavy load of sequence data.
Affiliation: Institute of Plant Genetics and Crop Plant Research (IPK), D-06466 Gatersleben, Germany; email@example.com.Show MeSH
Mentions: The MSC species tree (Fig. 2) resulted in a slightly different topology providing a better resolution for the closely related American species. The main disagreement concerned the three southern Patagonian species (H. comosum, H. patagonicum, and H. pubiflorum) found to be monophyletic and H. chilense/H. flexuosum as sister clade to the Patagonian clade. A second disagreement occurred with the Asian clade and the relationships within this clade where MSC provided high support (0.95 pp) for the monophyly of the clade but with H. roshevitzii outside although without any significant support within the clade (0.45 pp for H. brevisubulatum and H. roshevitzii). However, the differences are minor and do not influence the recognition of the major clades within Hordeum. In all analyses the recently proposed infrageneric taxonomic groups within Hordeum (Blattner 2009) were found to be monophyletic, for the first time with high support values (Fig. 1).
Affiliation: Institute of Plant Genetics and Crop Plant Research (IPK), D-06466 Gatersleben, Germany; firstname.lastname@example.org.