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Effect of Docosahexaenoic Acid on Apoptosis and Proliferation in the Placenta: Preliminary Report.

Wietrak E, Kamiński K, Leszczyńska-Gorzelak B, Oleszczuk J - Biomed Res Int (2015)

Bottom Line: The control group consisted of 50 women who did not receive such supplementation (control group).The expression of the proteins p21 and Ki-67 did not differ significantly between the groups.We did not find an association between DHA and proliferation process in the placenta.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Medical University of Lublin, Jaczewskiego 8 Street, 20-954 Lublin, Poland.

ABSTRACT

Introduction: Observational studies confirm a higher incidence of preeclampsia in patients with low erythrocyte concentrations of omega-3 fatty acids. Observations point to an association of disorders of pregnancy, such as intrauterine growth restriction (IUGR) and preeclampsia, with excessive apoptosis. One potential mechanism of action of docosahexaenoic acid (DHA) promoting a reduction in the risk of pathological pregnancy may be by influencing these processes in the placenta.

Materials and methods: We investigated 28 pregnant women supplemented with a fish oil product containing 300 mg DHA starting from pregnancy week 20 until delivery (DHA group). The control group consisted of 50 women who did not receive such supplementation (control group). We determined the expression of Ki-67 and p21 as markers of proliferation and caspase 3 activity as a marker of apoptosis and DHA levels in umbilical cord blood.

Results: Caspase 3 activity was significantly lower in the DHA group in comparison to the control group. Umbilical cord blood DHA concentration was higher in the DHA group. The expression of the proteins p21 and Ki-67 did not differ significantly between the groups.

Conclusions: We observed an association between DHA supplementation and inhibition of placental apoptosis. We did not find an association between DHA and proliferation process in the placenta.

No MeSH data available.


Related in: MedlinePlus

Average concentrations of DHA (ng/μL) in the serum of venous cord blood in both analyzed groups (P(U) = 0.0001).
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fig2: Average concentrations of DHA (ng/μL) in the serum of venous cord blood in both analyzed groups (P(U) = 0.0001).

Mentions: DHA concentration in the serum of venous cord blood differed significantly between the groups (P(U) < 0.0001). Cord blood DHA levels were higher in the DHA supplementation group (Figure 2). Overall, there was a highly significant negative rank correlation between cord blood DHA levels and the index of apoptosis (P < 0.01). Overall for all values we have Spearman's correlation −0,3082. For DHA group we received −0,0670 and for control group 0,0622, respectively. This finding means that as DHA levels increase in cord blood caspase 3 activity decreases (Figure 3). The correlation was calculated with Spearman's coefficient, which is robust for nonnormal distributions and outliers.


Effect of Docosahexaenoic Acid on Apoptosis and Proliferation in the Placenta: Preliminary Report.

Wietrak E, Kamiński K, Leszczyńska-Gorzelak B, Oleszczuk J - Biomed Res Int (2015)

Average concentrations of DHA (ng/μL) in the serum of venous cord blood in both analyzed groups (P(U) = 0.0001).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538367&req=5

fig2: Average concentrations of DHA (ng/μL) in the serum of venous cord blood in both analyzed groups (P(U) = 0.0001).
Mentions: DHA concentration in the serum of venous cord blood differed significantly between the groups (P(U) < 0.0001). Cord blood DHA levels were higher in the DHA supplementation group (Figure 2). Overall, there was a highly significant negative rank correlation between cord blood DHA levels and the index of apoptosis (P < 0.01). Overall for all values we have Spearman's correlation −0,3082. For DHA group we received −0,0670 and for control group 0,0622, respectively. This finding means that as DHA levels increase in cord blood caspase 3 activity decreases (Figure 3). The correlation was calculated with Spearman's coefficient, which is robust for nonnormal distributions and outliers.

Bottom Line: The control group consisted of 50 women who did not receive such supplementation (control group).The expression of the proteins p21 and Ki-67 did not differ significantly between the groups.We did not find an association between DHA and proliferation process in the placenta.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Medical University of Lublin, Jaczewskiego 8 Street, 20-954 Lublin, Poland.

ABSTRACT

Introduction: Observational studies confirm a higher incidence of preeclampsia in patients with low erythrocyte concentrations of omega-3 fatty acids. Observations point to an association of disorders of pregnancy, such as intrauterine growth restriction (IUGR) and preeclampsia, with excessive apoptosis. One potential mechanism of action of docosahexaenoic acid (DHA) promoting a reduction in the risk of pathological pregnancy may be by influencing these processes in the placenta.

Materials and methods: We investigated 28 pregnant women supplemented with a fish oil product containing 300 mg DHA starting from pregnancy week 20 until delivery (DHA group). The control group consisted of 50 women who did not receive such supplementation (control group). We determined the expression of Ki-67 and p21 as markers of proliferation and caspase 3 activity as a marker of apoptosis and DHA levels in umbilical cord blood.

Results: Caspase 3 activity was significantly lower in the DHA group in comparison to the control group. Umbilical cord blood DHA concentration was higher in the DHA group. The expression of the proteins p21 and Ki-67 did not differ significantly between the groups.

Conclusions: We observed an association between DHA supplementation and inhibition of placental apoptosis. We did not find an association between DHA and proliferation process in the placenta.

No MeSH data available.


Related in: MedlinePlus