Limits...
Effect of Docosahexaenoic Acid on Apoptosis and Proliferation in the Placenta: Preliminary Report.

Wietrak E, Kamiński K, Leszczyńska-Gorzelak B, Oleszczuk J - Biomed Res Int (2015)

Bottom Line: The control group consisted of 50 women who did not receive such supplementation (control group).The expression of the proteins p21 and Ki-67 did not differ significantly between the groups.We did not find an association between DHA and proliferation process in the placenta.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Medical University of Lublin, Jaczewskiego 8 Street, 20-954 Lublin, Poland.

ABSTRACT

Introduction: Observational studies confirm a higher incidence of preeclampsia in patients with low erythrocyte concentrations of omega-3 fatty acids. Observations point to an association of disorders of pregnancy, such as intrauterine growth restriction (IUGR) and preeclampsia, with excessive apoptosis. One potential mechanism of action of docosahexaenoic acid (DHA) promoting a reduction in the risk of pathological pregnancy may be by influencing these processes in the placenta.

Materials and methods: We investigated 28 pregnant women supplemented with a fish oil product containing 300 mg DHA starting from pregnancy week 20 until delivery (DHA group). The control group consisted of 50 women who did not receive such supplementation (control group). We determined the expression of Ki-67 and p21 as markers of proliferation and caspase 3 activity as a marker of apoptosis and DHA levels in umbilical cord blood.

Results: Caspase 3 activity was significantly lower in the DHA group in comparison to the control group. Umbilical cord blood DHA concentration was higher in the DHA group. The expression of the proteins p21 and Ki-67 did not differ significantly between the groups.

Conclusions: We observed an association between DHA supplementation and inhibition of placental apoptosis. We did not find an association between DHA and proliferation process in the placenta.

No MeSH data available.


Related in: MedlinePlus

Average activity of caspase 3 (μmol pNA/min/mL) in both analyzed groups (P(U) = 0.0001).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4538367&req=5

fig1: Average activity of caspase 3 (μmol pNA/min/mL) in both analyzed groups (P(U) = 0.0001).

Mentions: The statistical analysis of the data obtained in the supplementation versus control groups did not reveal statistically significant differences in the distribution of results with regard to age, duration of pregnancy, and body weight at birth between the groups. The supplementation and control groups were assumed to be comparable with regard to these indices. There were significant differences between the groups with regard to placental weight (P(U) = 0.0071), which was significantly higher in the control group. Apgar scores did not differ significantly between the groups (P(U) = 1.0000). The index of apoptosis, that is, caspase 3 activity, was significantly different between the groups (P(U) = 0.0001) (Figure 1): it was significantly lower in the DHA supplementation group as compared to the control group. The clinical details of the patients and their children and caspase 3 activity are presented in Table 1.


Effect of Docosahexaenoic Acid on Apoptosis and Proliferation in the Placenta: Preliminary Report.

Wietrak E, Kamiński K, Leszczyńska-Gorzelak B, Oleszczuk J - Biomed Res Int (2015)

Average activity of caspase 3 (μmol pNA/min/mL) in both analyzed groups (P(U) = 0.0001).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538367&req=5

fig1: Average activity of caspase 3 (μmol pNA/min/mL) in both analyzed groups (P(U) = 0.0001).
Mentions: The statistical analysis of the data obtained in the supplementation versus control groups did not reveal statistically significant differences in the distribution of results with regard to age, duration of pregnancy, and body weight at birth between the groups. The supplementation and control groups were assumed to be comparable with regard to these indices. There were significant differences between the groups with regard to placental weight (P(U) = 0.0071), which was significantly higher in the control group. Apgar scores did not differ significantly between the groups (P(U) = 1.0000). The index of apoptosis, that is, caspase 3 activity, was significantly different between the groups (P(U) = 0.0001) (Figure 1): it was significantly lower in the DHA supplementation group as compared to the control group. The clinical details of the patients and their children and caspase 3 activity are presented in Table 1.

Bottom Line: The control group consisted of 50 women who did not receive such supplementation (control group).The expression of the proteins p21 and Ki-67 did not differ significantly between the groups.We did not find an association between DHA and proliferation process in the placenta.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Medical University of Lublin, Jaczewskiego 8 Street, 20-954 Lublin, Poland.

ABSTRACT

Introduction: Observational studies confirm a higher incidence of preeclampsia in patients with low erythrocyte concentrations of omega-3 fatty acids. Observations point to an association of disorders of pregnancy, such as intrauterine growth restriction (IUGR) and preeclampsia, with excessive apoptosis. One potential mechanism of action of docosahexaenoic acid (DHA) promoting a reduction in the risk of pathological pregnancy may be by influencing these processes in the placenta.

Materials and methods: We investigated 28 pregnant women supplemented with a fish oil product containing 300 mg DHA starting from pregnancy week 20 until delivery (DHA group). The control group consisted of 50 women who did not receive such supplementation (control group). We determined the expression of Ki-67 and p21 as markers of proliferation and caspase 3 activity as a marker of apoptosis and DHA levels in umbilical cord blood.

Results: Caspase 3 activity was significantly lower in the DHA group in comparison to the control group. Umbilical cord blood DHA concentration was higher in the DHA group. The expression of the proteins p21 and Ki-67 did not differ significantly between the groups.

Conclusions: We observed an association between DHA supplementation and inhibition of placental apoptosis. We did not find an association between DHA and proliferation process in the placenta.

No MeSH data available.


Related in: MedlinePlus