Limits...
Bioconcentration and Acute Intoxication of Brazilian Freshwater Fishes by the Methyl Parathion Organophosphate Pesticide.

de Salles JB, Lopes RM, de Salles CM, Cassano VP, de Oliveira MM, Bastos VL, Bastos JC - Biomed Res Int (2015)

Bottom Line: The bioconcentration of methyl parathion was similar for all studied fishes.Three to 5 hours of 5 ppm methyl parathion exposure provoked the death of all P. lineatus at 92% brain AChE inhibition, whereas fish from the other two species survived for up to 78 hours with less than 80% brain AChE inhibition.Our results indicate that acute toxic effects of methyl parathion to fish are correlated with brain AChE sensitivity to methyl paraoxon.

View Article: PubMed Central - PubMed

Affiliation: Universidade Estadual da Zona Oeste, Rio de Janeiro, RJ, Brazil.

ABSTRACT
Three species of freshwater Brazilian fishes (pacu, Piaractus mesopotamicus; piavussu, Leporinus macrocephalus, and curimbatá, Prochilodus lineatus) were exposed to an acute dose of 5 ppm methyl parathion organophosphate pesticide. Three to five individuals per species were exposed, one at a time, to 40 liters tap water spiked with Folidol 600. Pesticide concentrations and cholinesterase (ChE) activities were evaluated in serum, liver, brain, heart, and muscle. The bioconcentration of methyl parathion was similar for all studied fishes. Brain tissue showed the highest pesticide concentration, reaching 80 ppm after exposure for 30 min to methyl parathion. Three to 5 hours of 5 ppm methyl parathion exposure provoked the death of all P. lineatus at 92% brain AChE inhibition, whereas fish from the other two species survived for up to 78 hours with less than 80% brain AChE inhibition. Our results indicate that acute toxic effects of methyl parathion to fish are correlated with brain AChE sensitivity to methyl paraoxon.

No MeSH data available.


Related in: MedlinePlus

Cholinesterase activity in tissues of pacu, piavussu, and curimbatá exposed to 5 ppm methyl parathion. Each one of eight animals by species was individually treated with Folidol 600 for the indicated time in a 40 L aquarium. After 30 min of exposure blood was collected from four fishes by species and these fishes were submitted to euthanasia. Then, of the remaining fish, four pacus and four piavussus were euthanized after 78 h exposure, while four curimbatás were collected immediately after they showed no movement of opercula (from 3 to 5 h of exposure). Tissues were dissected and homogenized. Cholinesterase was assayed in serum and homogenates with acetylthiocholine and expressed as percentage of the activity found in controls.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4538366&req=5

fig4: Cholinesterase activity in tissues of pacu, piavussu, and curimbatá exposed to 5 ppm methyl parathion. Each one of eight animals by species was individually treated with Folidol 600 for the indicated time in a 40 L aquarium. After 30 min of exposure blood was collected from four fishes by species and these fishes were submitted to euthanasia. Then, of the remaining fish, four pacus and four piavussus were euthanized after 78 h exposure, while four curimbatás were collected immediately after they showed no movement of opercula (from 3 to 5 h of exposure). Tissues were dissected and homogenized. Cholinesterase was assayed in serum and homogenates with acetylthiocholine and expressed as percentage of the activity found in controls.

Mentions: Heart, liver, and serum cholinesterase activities for pacu, piavussu, and curimbatá were greatly inhibited after 30 minutes exposure to Folidol. By contrast, skeletal muscle ChE activity for the three species and brain AChE activity for pacu showed no inhibition within this time period (Figure 4). Exposure of fishes to water with Folidol for 78 h resulted in 70% brain AChE activity inhibition in pacu and 60% brain AChE activity inhibition in piavussu.


Bioconcentration and Acute Intoxication of Brazilian Freshwater Fishes by the Methyl Parathion Organophosphate Pesticide.

de Salles JB, Lopes RM, de Salles CM, Cassano VP, de Oliveira MM, Bastos VL, Bastos JC - Biomed Res Int (2015)

Cholinesterase activity in tissues of pacu, piavussu, and curimbatá exposed to 5 ppm methyl parathion. Each one of eight animals by species was individually treated with Folidol 600 for the indicated time in a 40 L aquarium. After 30 min of exposure blood was collected from four fishes by species and these fishes were submitted to euthanasia. Then, of the remaining fish, four pacus and four piavussus were euthanized after 78 h exposure, while four curimbatás were collected immediately after they showed no movement of opercula (from 3 to 5 h of exposure). Tissues were dissected and homogenized. Cholinesterase was assayed in serum and homogenates with acetylthiocholine and expressed as percentage of the activity found in controls.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538366&req=5

fig4: Cholinesterase activity in tissues of pacu, piavussu, and curimbatá exposed to 5 ppm methyl parathion. Each one of eight animals by species was individually treated with Folidol 600 for the indicated time in a 40 L aquarium. After 30 min of exposure blood was collected from four fishes by species and these fishes were submitted to euthanasia. Then, of the remaining fish, four pacus and four piavussus were euthanized after 78 h exposure, while four curimbatás were collected immediately after they showed no movement of opercula (from 3 to 5 h of exposure). Tissues were dissected and homogenized. Cholinesterase was assayed in serum and homogenates with acetylthiocholine and expressed as percentage of the activity found in controls.
Mentions: Heart, liver, and serum cholinesterase activities for pacu, piavussu, and curimbatá were greatly inhibited after 30 minutes exposure to Folidol. By contrast, skeletal muscle ChE activity for the three species and brain AChE activity for pacu showed no inhibition within this time period (Figure 4). Exposure of fishes to water with Folidol for 78 h resulted in 70% brain AChE activity inhibition in pacu and 60% brain AChE activity inhibition in piavussu.

Bottom Line: The bioconcentration of methyl parathion was similar for all studied fishes.Three to 5 hours of 5 ppm methyl parathion exposure provoked the death of all P. lineatus at 92% brain AChE inhibition, whereas fish from the other two species survived for up to 78 hours with less than 80% brain AChE inhibition.Our results indicate that acute toxic effects of methyl parathion to fish are correlated with brain AChE sensitivity to methyl paraoxon.

View Article: PubMed Central - PubMed

Affiliation: Universidade Estadual da Zona Oeste, Rio de Janeiro, RJ, Brazil.

ABSTRACT
Three species of freshwater Brazilian fishes (pacu, Piaractus mesopotamicus; piavussu, Leporinus macrocephalus, and curimbatá, Prochilodus lineatus) were exposed to an acute dose of 5 ppm methyl parathion organophosphate pesticide. Three to five individuals per species were exposed, one at a time, to 40 liters tap water spiked with Folidol 600. Pesticide concentrations and cholinesterase (ChE) activities were evaluated in serum, liver, brain, heart, and muscle. The bioconcentration of methyl parathion was similar for all studied fishes. Brain tissue showed the highest pesticide concentration, reaching 80 ppm after exposure for 30 min to methyl parathion. Three to 5 hours of 5 ppm methyl parathion exposure provoked the death of all P. lineatus at 92% brain AChE inhibition, whereas fish from the other two species survived for up to 78 hours with less than 80% brain AChE inhibition. Our results indicate that acute toxic effects of methyl parathion to fish are correlated with brain AChE sensitivity to methyl paraoxon.

No MeSH data available.


Related in: MedlinePlus