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Bioconcentration and Acute Intoxication of Brazilian Freshwater Fishes by the Methyl Parathion Organophosphate Pesticide.

de Salles JB, Lopes RM, de Salles CM, Cassano VP, de Oliveira MM, Bastos VL, Bastos JC - Biomed Res Int (2015)

Bottom Line: The bioconcentration of methyl parathion was similar for all studied fishes.Three to 5 hours of 5 ppm methyl parathion exposure provoked the death of all P. lineatus at 92% brain AChE inhibition, whereas fish from the other two species survived for up to 78 hours with less than 80% brain AChE inhibition.Our results indicate that acute toxic effects of methyl parathion to fish are correlated with brain AChE sensitivity to methyl paraoxon.

View Article: PubMed Central - PubMed

Affiliation: Universidade Estadual da Zona Oeste, Rio de Janeiro, RJ, Brazil.

ABSTRACT
Three species of freshwater Brazilian fishes (pacu, Piaractus mesopotamicus; piavussu, Leporinus macrocephalus, and curimbatá, Prochilodus lineatus) were exposed to an acute dose of 5 ppm methyl parathion organophosphate pesticide. Three to five individuals per species were exposed, one at a time, to 40 liters tap water spiked with Folidol 600. Pesticide concentrations and cholinesterase (ChE) activities were evaluated in serum, liver, brain, heart, and muscle. The bioconcentration of methyl parathion was similar for all studied fishes. Brain tissue showed the highest pesticide concentration, reaching 80 ppm after exposure for 30 min to methyl parathion. Three to 5 hours of 5 ppm methyl parathion exposure provoked the death of all P. lineatus at 92% brain AChE inhibition, whereas fish from the other two species survived for up to 78 hours with less than 80% brain AChE inhibition. Our results indicate that acute toxic effects of methyl parathion to fish are correlated with brain AChE sensitivity to methyl paraoxon.

No MeSH data available.


Related in: MedlinePlus

Concentration of methyl parathion in muscle, heart, brain, and liver and serum from pacu, piavussu, and curimbatá. Each one of four animals per species was placed in separated tanks containing 40 L of water with Folidol to produce 5 ppm methyl parathion. After 30 minutes exposure, blood samples were collected and the fishes were euthanized. Tissues were dissected and homogenized. Methyl parathion was extracted from serum and tissue homogenates and quantified by HPLC.
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fig2: Concentration of methyl parathion in muscle, heart, brain, and liver and serum from pacu, piavussu, and curimbatá. Each one of four animals per species was placed in separated tanks containing 40 L of water with Folidol to produce 5 ppm methyl parathion. After 30 minutes exposure, blood samples were collected and the fishes were euthanized. Tissues were dissected and homogenized. Methyl parathion was extracted from serum and tissue homogenates and quantified by HPLC.

Mentions: Muscle, heart, brain, liver, and serum from pacu, piavussu, and curimbatá exhibited similar methyl parathion bioconcentration patterns (Figure 2). Brain tissue showed the highest capacity for methyl parathion bioconcentration, reaching 80 ppm (16-fold increase) after 30 minutes exposure to methyl parathion in water (Figure 2). The maximum methyl parathion concentration in muscle, heart, brain, and serum was achieved after 30 minutes, with one exception: methyl parathion concentrations in piavussu showed an increase after 24 hours exposure, reaching a maximum of 120 ppm in liver, corresponding to a 24-fold increase (Figure 3). Methyl parathion was not found in samples from control animals.


Bioconcentration and Acute Intoxication of Brazilian Freshwater Fishes by the Methyl Parathion Organophosphate Pesticide.

de Salles JB, Lopes RM, de Salles CM, Cassano VP, de Oliveira MM, Bastos VL, Bastos JC - Biomed Res Int (2015)

Concentration of methyl parathion in muscle, heart, brain, and liver and serum from pacu, piavussu, and curimbatá. Each one of four animals per species was placed in separated tanks containing 40 L of water with Folidol to produce 5 ppm methyl parathion. After 30 minutes exposure, blood samples were collected and the fishes were euthanized. Tissues were dissected and homogenized. Methyl parathion was extracted from serum and tissue homogenates and quantified by HPLC.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538366&req=5

fig2: Concentration of methyl parathion in muscle, heart, brain, and liver and serum from pacu, piavussu, and curimbatá. Each one of four animals per species was placed in separated tanks containing 40 L of water with Folidol to produce 5 ppm methyl parathion. After 30 minutes exposure, blood samples were collected and the fishes were euthanized. Tissues were dissected and homogenized. Methyl parathion was extracted from serum and tissue homogenates and quantified by HPLC.
Mentions: Muscle, heart, brain, liver, and serum from pacu, piavussu, and curimbatá exhibited similar methyl parathion bioconcentration patterns (Figure 2). Brain tissue showed the highest capacity for methyl parathion bioconcentration, reaching 80 ppm (16-fold increase) after 30 minutes exposure to methyl parathion in water (Figure 2). The maximum methyl parathion concentration in muscle, heart, brain, and serum was achieved after 30 minutes, with one exception: methyl parathion concentrations in piavussu showed an increase after 24 hours exposure, reaching a maximum of 120 ppm in liver, corresponding to a 24-fold increase (Figure 3). Methyl parathion was not found in samples from control animals.

Bottom Line: The bioconcentration of methyl parathion was similar for all studied fishes.Three to 5 hours of 5 ppm methyl parathion exposure provoked the death of all P. lineatus at 92% brain AChE inhibition, whereas fish from the other two species survived for up to 78 hours with less than 80% brain AChE inhibition.Our results indicate that acute toxic effects of methyl parathion to fish are correlated with brain AChE sensitivity to methyl paraoxon.

View Article: PubMed Central - PubMed

Affiliation: Universidade Estadual da Zona Oeste, Rio de Janeiro, RJ, Brazil.

ABSTRACT
Three species of freshwater Brazilian fishes (pacu, Piaractus mesopotamicus; piavussu, Leporinus macrocephalus, and curimbatá, Prochilodus lineatus) were exposed to an acute dose of 5 ppm methyl parathion organophosphate pesticide. Three to five individuals per species were exposed, one at a time, to 40 liters tap water spiked with Folidol 600. Pesticide concentrations and cholinesterase (ChE) activities were evaluated in serum, liver, brain, heart, and muscle. The bioconcentration of methyl parathion was similar for all studied fishes. Brain tissue showed the highest pesticide concentration, reaching 80 ppm after exposure for 30 min to methyl parathion. Three to 5 hours of 5 ppm methyl parathion exposure provoked the death of all P. lineatus at 92% brain AChE inhibition, whereas fish from the other two species survived for up to 78 hours with less than 80% brain AChE inhibition. Our results indicate that acute toxic effects of methyl parathion to fish are correlated with brain AChE sensitivity to methyl paraoxon.

No MeSH data available.


Related in: MedlinePlus