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Bioconcentration and Acute Intoxication of Brazilian Freshwater Fishes by the Methyl Parathion Organophosphate Pesticide.

de Salles JB, Lopes RM, de Salles CM, Cassano VP, de Oliveira MM, Bastos VL, Bastos JC - Biomed Res Int (2015)

Bottom Line: The bioconcentration of methyl parathion was similar for all studied fishes.Three to 5 hours of 5 ppm methyl parathion exposure provoked the death of all P. lineatus at 92% brain AChE inhibition, whereas fish from the other two species survived for up to 78 hours with less than 80% brain AChE inhibition.Our results indicate that acute toxic effects of methyl parathion to fish are correlated with brain AChE sensitivity to methyl paraoxon.

View Article: PubMed Central - PubMed

Affiliation: Universidade Estadual da Zona Oeste, Rio de Janeiro, RJ, Brazil.

ABSTRACT
Three species of freshwater Brazilian fishes (pacu, Piaractus mesopotamicus; piavussu, Leporinus macrocephalus, and curimbatá, Prochilodus lineatus) were exposed to an acute dose of 5 ppm methyl parathion organophosphate pesticide. Three to five individuals per species were exposed, one at a time, to 40 liters tap water spiked with Folidol 600. Pesticide concentrations and cholinesterase (ChE) activities were evaluated in serum, liver, brain, heart, and muscle. The bioconcentration of methyl parathion was similar for all studied fishes. Brain tissue showed the highest pesticide concentration, reaching 80 ppm after exposure for 30 min to methyl parathion. Three to 5 hours of 5 ppm methyl parathion exposure provoked the death of all P. lineatus at 92% brain AChE inhibition, whereas fish from the other two species survived for up to 78 hours with less than 80% brain AChE inhibition. Our results indicate that acute toxic effects of methyl parathion to fish are correlated with brain AChE sensitivity to methyl paraoxon.

No MeSH data available.


Related in: MedlinePlus

Tissue distribution of cholinesterase activity in pacu, piavussu and curimbatá (control fish). The box shows the substrates used in enzyme assays. Results are expressed as μmol of products formed per minute per gram of wet tissue (U g−1) or per mL of serum (U mL−1). Liver and brain ChE activities were assayed in homogenates, while heart and muscle ChE activities were assayed in the enzymatic fraction solubilized with Triton and NaCl. Results are average values with the corresponding SEM of assays carried out in five individuals of each species.
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fig1: Tissue distribution of cholinesterase activity in pacu, piavussu and curimbatá (control fish). The box shows the substrates used in enzyme assays. Results are expressed as μmol of products formed per minute per gram of wet tissue (U g−1) or per mL of serum (U mL−1). Liver and brain ChE activities were assayed in homogenates, while heart and muscle ChE activities were assayed in the enzymatic fraction solubilized with Triton and NaCl. Results are average values with the corresponding SEM of assays carried out in five individuals of each species.

Mentions: The studied fish species (control groups) exhibited remarkable differences in tissue-specific cholinesterase activity (Figure 1). The ChE activity measured in the serum of curimbatá and pacu specimens was lower than the activity measured in brain, liver, heart, and muscle. By contrast, in serum of piavussu the ChE activity was similar to that measured in liver, brain, heart, and muscle.


Bioconcentration and Acute Intoxication of Brazilian Freshwater Fishes by the Methyl Parathion Organophosphate Pesticide.

de Salles JB, Lopes RM, de Salles CM, Cassano VP, de Oliveira MM, Bastos VL, Bastos JC - Biomed Res Int (2015)

Tissue distribution of cholinesterase activity in pacu, piavussu and curimbatá (control fish). The box shows the substrates used in enzyme assays. Results are expressed as μmol of products formed per minute per gram of wet tissue (U g−1) or per mL of serum (U mL−1). Liver and brain ChE activities were assayed in homogenates, while heart and muscle ChE activities were assayed in the enzymatic fraction solubilized with Triton and NaCl. Results are average values with the corresponding SEM of assays carried out in five individuals of each species.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538366&req=5

fig1: Tissue distribution of cholinesterase activity in pacu, piavussu and curimbatá (control fish). The box shows the substrates used in enzyme assays. Results are expressed as μmol of products formed per minute per gram of wet tissue (U g−1) or per mL of serum (U mL−1). Liver and brain ChE activities were assayed in homogenates, while heart and muscle ChE activities were assayed in the enzymatic fraction solubilized with Triton and NaCl. Results are average values with the corresponding SEM of assays carried out in five individuals of each species.
Mentions: The studied fish species (control groups) exhibited remarkable differences in tissue-specific cholinesterase activity (Figure 1). The ChE activity measured in the serum of curimbatá and pacu specimens was lower than the activity measured in brain, liver, heart, and muscle. By contrast, in serum of piavussu the ChE activity was similar to that measured in liver, brain, heart, and muscle.

Bottom Line: The bioconcentration of methyl parathion was similar for all studied fishes.Three to 5 hours of 5 ppm methyl parathion exposure provoked the death of all P. lineatus at 92% brain AChE inhibition, whereas fish from the other two species survived for up to 78 hours with less than 80% brain AChE inhibition.Our results indicate that acute toxic effects of methyl parathion to fish are correlated with brain AChE sensitivity to methyl paraoxon.

View Article: PubMed Central - PubMed

Affiliation: Universidade Estadual da Zona Oeste, Rio de Janeiro, RJ, Brazil.

ABSTRACT
Three species of freshwater Brazilian fishes (pacu, Piaractus mesopotamicus; piavussu, Leporinus macrocephalus, and curimbatá, Prochilodus lineatus) were exposed to an acute dose of 5 ppm methyl parathion organophosphate pesticide. Three to five individuals per species were exposed, one at a time, to 40 liters tap water spiked with Folidol 600. Pesticide concentrations and cholinesterase (ChE) activities were evaluated in serum, liver, brain, heart, and muscle. The bioconcentration of methyl parathion was similar for all studied fishes. Brain tissue showed the highest pesticide concentration, reaching 80 ppm after exposure for 30 min to methyl parathion. Three to 5 hours of 5 ppm methyl parathion exposure provoked the death of all P. lineatus at 92% brain AChE inhibition, whereas fish from the other two species survived for up to 78 hours with less than 80% brain AChE inhibition. Our results indicate that acute toxic effects of methyl parathion to fish are correlated with brain AChE sensitivity to methyl paraoxon.

No MeSH data available.


Related in: MedlinePlus