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miR-965 controls cell proliferation and migration during tissue morphogenesis in the Drosophila abdomen.

Verma P, Cohen SM - Elife (2015)

Bottom Line: During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis.Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis.By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular and Cell Biology, Singapore, Singapore.

ABSTRACT
Formation of the Drosophila adult abdomen involves a process of tissue replacement in which larval epidermal cells are replaced by adult cells. The progenitors of the adult epidermis are specified during embryogenesis and, unlike the imaginal discs that make up the thoracic and head segments, they remain quiescent during larval development. During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis. Here, we provide evidence that the microRNA, miR-965, acts via string and wingless to control histoblast proliferation and migration. Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis. Replacement of the larval epidermis by adult epidermal progenitors involves regulation of both cell-intrinsic events and cell communication. By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

No MeSH data available.


Related in: MedlinePlus

Speed of histoblast migration restored by reduced string activity.Left: speed of histoblast nest migration in the third abdominal segment. p < 0.05 comparing KO1/KO2 with KO1/KO2; stgEY12388. Control (n = 15), KO1/KO2 (n = 18) and KO1/KO2; stgEY12388 (n = 14). The Control and KO1/KO2 samples are the same as those in Figure 3—figure supplement 1. The two experiments were done together. Right: speed of histoblast migration in the fourth abdominal segment. p < 0.05 comparing KO1/KO2 with KO1/KO2; stgEY12388. Refers to Figure 5D and Video 11.DOI:http://dx.doi.org/10.7554/eLife.07389.026
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fig5s5: Speed of histoblast migration restored by reduced string activity.Left: speed of histoblast nest migration in the third abdominal segment. p < 0.05 comparing KO1/KO2 with KO1/KO2; stgEY12388. Control (n = 15), KO1/KO2 (n = 18) and KO1/KO2; stgEY12388 (n = 14). The Control and KO1/KO2 samples are the same as those in Figure 3—figure supplement 1. The two experiments were done together. Right: speed of histoblast migration in the fourth abdominal segment. p < 0.05 comparing KO1/KO2 with KO1/KO2; stgEY12388. Refers to Figure 5D and Video 11.DOI:http://dx.doi.org/10.7554/eLife.07389.026


miR-965 controls cell proliferation and migration during tissue morphogenesis in the Drosophila abdomen.

Verma P, Cohen SM - Elife (2015)

Speed of histoblast migration restored by reduced string activity.Left: speed of histoblast nest migration in the third abdominal segment. p < 0.05 comparing KO1/KO2 with KO1/KO2; stgEY12388. Control (n = 15), KO1/KO2 (n = 18) and KO1/KO2; stgEY12388 (n = 14). The Control and KO1/KO2 samples are the same as those in Figure 3—figure supplement 1. The two experiments were done together. Right: speed of histoblast migration in the fourth abdominal segment. p < 0.05 comparing KO1/KO2 with KO1/KO2; stgEY12388. Refers to Figure 5D and Video 11.DOI:http://dx.doi.org/10.7554/eLife.07389.026
© Copyright Policy
Related In: Results  -  Collection

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fig5s5: Speed of histoblast migration restored by reduced string activity.Left: speed of histoblast nest migration in the third abdominal segment. p < 0.05 comparing KO1/KO2 with KO1/KO2; stgEY12388. Control (n = 15), KO1/KO2 (n = 18) and KO1/KO2; stgEY12388 (n = 14). The Control and KO1/KO2 samples are the same as those in Figure 3—figure supplement 1. The two experiments were done together. Right: speed of histoblast migration in the fourth abdominal segment. p < 0.05 comparing KO1/KO2 with KO1/KO2; stgEY12388. Refers to Figure 5D and Video 11.DOI:http://dx.doi.org/10.7554/eLife.07389.026
Bottom Line: During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis.Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis.By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular and Cell Biology, Singapore, Singapore.

ABSTRACT
Formation of the Drosophila adult abdomen involves a process of tissue replacement in which larval epidermal cells are replaced by adult cells. The progenitors of the adult epidermis are specified during embryogenesis and, unlike the imaginal discs that make up the thoracic and head segments, they remain quiescent during larval development. During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis. Here, we provide evidence that the microRNA, miR-965, acts via string and wingless to control histoblast proliferation and migration. Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis. Replacement of the larval epidermis by adult epidermal progenitors involves regulation of both cell-intrinsic events and cell communication. By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

No MeSH data available.


Related in: MedlinePlus